Search found 2778 matches

by zzffnn
Tue Jun 01, 2021 11:54 pm
Forum: Illumination Techniques
Topic: Dark Field Condenser vs DYI Filter/Patch
Replies: 13
Views: 295

Re: Dark Field Condenser vs DYI Filter/Patch

DIY darkfield patches do not work well above NA 0.65 (usually 40x objective, below that patches work just fine).

Dedicated darkfield condenser has mirrored internal surface for better light efficiency and can also provide better optical correction.
by zzffnn
Mon May 03, 2021 7:04 pm
Forum: Specimens, samples and slides
Topic: Round slides?
Replies: 7
Views: 327

Re: Round slides?

Edit: This eBay listing offers multiple sizes: https://www.ebay.com/itm/274135982428?_trkparms=aid%3D1110006%26algo%3DHOMESPLICE.SIM%26ao%3D1%26asc%3D20191002094254%26meid%3D0373bd4f1f334cc391c64d7469ed434f%26pid%3D101103%26rk%3D4%26rkt%3D6%26sd%3D274204314544%26itm%3D274135982428%26pmt%3D0%26noa%3D...
by zzffnn
Fri Apr 30, 2021 9:08 am
Forum: My microscope
Topic: Feeling a bit lost (buying advice / Belgium)
Replies: 25
Views: 1166

Re: Feeling a bit lost (buying advice / Belgium)

If you live close to a local university or research hospital, they may sell good used microscopes as surplus, at local auctions.
by zzffnn
Tue Apr 20, 2021 4:46 pm
Forum: Microscopes and optics
Topic: Most useful magnifications for phase contrast
Replies: 13
Views: 728

Re: Most useful magnifications for phase contrast

It is very personal and depends on your preference and subjects of interest. For me, my most useful phase contrast objective is 100x. Because at up to 20x NA 0.4-0.5 or 40x NA 0.65, I vastly prefer DIY darkfield, dedicated immersion darkfield (or DIC) for my protists viewing, over phase contrast. At...
by zzffnn
Mon Apr 19, 2021 5:44 am
Forum: My microscope
Topic: Thanks for some good advice
Replies: 3
Views: 376

Re: Thanks for some good advice

:mrgreen: Now we expect to see some colors, Mr. Schaeffer!
by zzffnn
Mon Apr 19, 2021 2:16 am
Forum: Introduce yourself
Topic: Amateur Marine Scientist Looking for Advice
Replies: 1
Views: 208

Re: Amateur Marine Scientist Looking for Advice

Welcome, ReefLabs! I am a seashell collector, microbiologist and I used to keep a tropical aquarium.

Please feel free to ask, if you have specific questions in mind regarding microscopy.
by zzffnn
Sat Apr 17, 2021 7:25 am
Forum: Pictures and Videos
Topic: Among the Growing Roots
Replies: 8
Views: 489

Re: Among the Growing Roots

Don, Here is a video with color that I took 5 years ago under a 70x NA 1.23 objective, without any software modification. You are certainly right in that its color reproduction is not as vibrant as a good 10x: https://youtu.be/a1BYrzTF60M However, we should see at least some green (chlorophyll), bro...
by zzffnn
Thu Apr 15, 2021 10:25 pm
Forum: Pictures and Videos
Topic: Among the Growing Roots
Replies: 8
Views: 489

Re: Among the Growing Roots

Don,

Sorry to be so blunt, however: there should be some colors in those subjects you recorded in videos.

I think your condenser may be set too low; or condenser iris / illuminator field iris closed down too much.

I like your poems and enthusiasm, by the way!

Sincerely,
Frank
by zzffnn
Thu Apr 15, 2021 9:47 pm
Forum: Microscopes and optics
Topic: PZO DIC on a BHT microscope, possible?
Replies: 50
Views: 2674

Re: PZO DIC on a BHT microscope, possible?

Today was a good day, I received both the KIP2 which looks new and also a few Nikon PlanApos... :D These are preliminary tests (I smashed the poor thing); condenser just placed on top fo the BHT condenser holder, MIP5 far from centred and system not parfocal. I tested the CFN 10, 20 and 40 planApos...
by zzffnn
Mon Apr 12, 2021 11:50 am
Forum: Microscopes and optics
Topic: PZO DIC on a BHT microscope, possible?
Replies: 50
Views: 2674

Re: PZO DIC on a BHT microscope, possible?

Great idea, thank you for your kind advice, Javier.

Yes, I meant 43mm for PZO. Forgive me brain fart.
by zzffnn
Mon Apr 12, 2021 6:50 am
Forum: Microscopes and optics
Topic: PZO DIC on a BHT microscope, possible?
Replies: 50
Views: 2674

Re: PZO DIC on a BHT microscope, possible?

So PZO head dovetail is around 44mm, since Olympus head dovetail is 42mm? That is not good news for a Nikon owner like me, who has 48.5mm head dovetails. If one adapter adds 8mm tube length and I need two, that means 16mm tube length increase! I think I wouldn't use adapter for my scope then, I can ...
by zzffnn
Sun Apr 11, 2021 2:39 am
Forum: Microscopes and optics
Topic: PZO DIC on a BHT microscope, possible?
Replies: 50
Views: 2674

Re: PZO DIC on a BHT microscope, possible?

Can MIP5's dovetail be shimmed up or down to fit Olympus arm? Maybe add a few small pieces of wood / aluminum foil or something? 2mm doesn't sound a lot, if MIP5 is smaller (sorry, I haven't followed up carefully to know if MIP5 is bigger or smaller for Olympus). I am guessing one can sand off 1mm f...
by zzffnn
Sun Apr 11, 2021 12:54 am
Forum: Microscopes and optics
Topic: PZO DIC on a BHT microscope, possible?
Replies: 50
Views: 2674

Re: PZO DIC on a BHT microscope, possible?

It depends on what (tube length) you have after the MIP5.

If it is an unmodified Olympus trinocular head, then tube length should be perfect.

Or you can use extension tubes of the right length for camera only and sacrifice visual viewing from visual eyepieces.
by zzffnn
Sat Apr 10, 2021 12:25 am
Forum: Microscopes and optics
Topic: PZO DIC on a BHT microscope, possible?
Replies: 50
Views: 2674

Re: PZO DIC on a BHT microscope, possible?

synnus wrote:
Fri Apr 09, 2021 11:42 pm
I think pzo dic can fit bh2
https://youtu.be/TQbKw3CeZ58
Very nice. Did you use a condenser dovetail adapter for using KPI2 on BH2?
by zzffnn
Fri Apr 09, 2021 7:31 pm
Forum: Microscopes and optics
Topic: PZO DIC on a BHT microscope, possible?
Replies: 50
Views: 2674

Re: PZO DIC on a BHT microscope, possible?

Found a KPI2 like new, 210€; a bit under 600€ with the MIP5 Seller may have some more if someone interested, ships from Poland Saul made me an adapter for the MIP5 to the BHT microscope, I will use just tubes on top of it with the camera to be completely parfocal Looking forward to put it to work.....
by zzffnn
Thu Apr 08, 2021 9:12 am
Forum: Illumination Techniques
Topic: DIY phase anulus with LOMO phase objectiv
Replies: 13
Views: 997

Re: DIY phase anulus with LOMO phase objectiv

If phase annulus is moved closer to condenser bottom lens (for example it can be stuck there using double-sided tape), DIY phase images should improve further.
by zzffnn
Tue Apr 06, 2021 4:13 am
Forum: Home-made microscope adaptations
Topic: Have 3d printer, will print.
Replies: 36
Views: 3454

Re: Have 3d printer, will print.

I would be happy to buy such a variable reversed aperture as well. I had Leitz Heine before. It has internal mirror walls that reflect light and corrects distortions. A simple hollow light ring would only block light and won't work as well as Heine, but it should work, assuming light source is powef...
by zzffnn
Tue Apr 06, 2021 3:58 am
Forum: Specimens, samples and slides
Topic: Using aluminium tape to make spacers for water sample specimens
Replies: 11
Views: 834

Re: Using aluminium tape to make spacers for water sample specimens

Many people use petroleum jelly, because you can press it to make it thinner and it kind of prevents water from leaking.

Aluminum foil for oven cooking, transparent tape, plastic fil wraps, may also work, depending on your sample thickness.
by zzffnn
Wed Mar 24, 2021 10:20 pm
Forum: Specimens, samples and slides
Topic: How to explain it?
Replies: 16
Views: 1160

Re: How to explain it?

I don't know if these count:

1) the fact that water bears are nearly immortal;

2) how cancer cells can survive most (several lines of treatments) of the modern therapeutics.

These are research and theories on those. But we are not completely clear yet.
by zzffnn
Wed Mar 24, 2021 9:11 pm
Forum: Pictures and Videos
Topic: Darkfield through glass edge illumination - Coleps
Replies: 27
Views: 1536

Re: Darkfield through glass edge illumination - Coleps

Saul, you bera version looks very promising, have you tested it on a mounted diatom, for example Pleurosigma or Gyrosigma species?
by zzffnn
Wed Mar 24, 2021 9:07 pm
Forum: Camera systems and imaging
Topic: Looking for a good m4/3 camera for focus stacking
Replies: 10
Views: 576

Re: Looking for a good m4/3 camera for focus stacking

Hans, I did compared E-M1 on the same subject at the same time, full electronic shutter vs EFCS. Then pixel peeped. EFCS resolved quite a bit better. Full electronic shutter seemed to produce much more noise grains, at the same iso. I have Panasonic G85 before, did the same test and did NOT see a si...
by zzffnn
Wed Mar 24, 2021 9:02 pm
Forum: Camera systems and imaging
Topic: Looking for a good m4/3 camera for focus stacking
Replies: 10
Views: 576

Re: Looking for a good m4/3 camera for focus stacking

Javier, that crop ratio sounds about right. If your current mirrorless APSC camera works well, there is probably no point to change it. My Nikon Optiphot did get shaken at high magnification by my camera's 2nd mechanical shutter; I saw it in flipped up camera LCD. But my water mount was not too thin...
by zzffnn
Wed Mar 24, 2021 4:12 pm
Forum: Camera systems and imaging
Topic: Looking for a good m4/3 camera for focus stacking
Replies: 10
Views: 576

Re: Looking for a good m4/3 camera for focus stacking

Do not get Olympus E-M1 mk1. Its images are noticeably worse in full electronic shutter mode, than EFSC. Focus stacking with EFSC won't work very well, if your subject is in thick water mount under high magnification. Even that 2nd shutter shock may (or may not) move the subject and ruin the next im...
by zzffnn
Wed Mar 24, 2021 4:05 pm
Forum: Pictures and Videos
Topic: Darkfield through glass edge illumination - Coleps
Replies: 27
Views: 1536

Re: Darkfield through glass edge illumination - Coleps

For those edge illumination darkfield, has any of you examined objective back focal plane to make sure objective aperture is sufficiently illuminated by peripheral circular light? I apologize if I sound discouraging or harsh. Some of the higher NA (0.65 and above) images presented here look as if ob...
by zzffnn
Sun Mar 21, 2021 9:31 pm
Forum: Microscopes and optics
Topic: Parfocal extender
Replies: 4
Views: 426

Re: Parfocal extender

Giulio, I have used those short 32.7mm LOMO objectives on Nikon Labophot 2 and Optiphot 1. I usually have a full turret of 5 short LOMO apo objectives on the same turret. Instead of using parfocal extenders (which significantly degrades high NA objective performance), I raised up my Labophot / Optip...
by zzffnn
Thu Mar 18, 2021 11:10 pm
Forum: Microscopes and optics
Topic: PZO DIC on a BHT microscope, possible?
Replies: 50
Views: 2674

Re: PZO DIC on a BHT microscope, possible?

Are you sure your tube length is off (why would it be off, since MIP5 compensates its added own tube length)? I would think it is right on at 160mm, as long as your original (without PZO) tube length is correct. 1.25x magnifier intermefiate piece should go after the MIP-5, I would guess, although I ...
by zzffnn
Thu Mar 18, 2021 8:48 pm
Forum: Microscopes and optics
Topic: PZO DIC on a BHT microscope, possible?
Replies: 50
Views: 2674

Re: PZO DIC on a BHT microscope, possible?

Do not modify the MIP5 unit any further, for now. Quite a few things sound unusual there (the metal piece inside, the decentering and the ghosting). Is your seller reputable? I would suggest you also post at the other forum to ask: http://www.photomacrography.net/forum/viewforum.php?f=25&sid=6af6d6b...
by zzffnn
Mon Mar 15, 2021 5:07 pm
Forum: Miscellaneous
Topic: Mental struggles and microscopy
Replies: 24
Views: 2583

Re: Mental struggles and microscopy

I have a close friend with attention-deficit hyperactivity disorder (ADHD) and cancer, who inspires me significantly and changed how I perceived others and my own life. I myself had mild depression a few years back and have since learned about psychology. Our brains know how to compensate; there is ...
by zzffnn
Mon Mar 15, 2021 2:37 pm
Forum: Introduce yourself
Topic: Forum Newbie from Connecticut
Replies: 2
Views: 264

Re: Forum Newbie from Connecticut

Welcome from Beantown, Voyager!
by zzffnn
Mon Mar 15, 2021 2:32 am
Forum: Introduce yourself
Topic: Hello and any pre-purchase 2-cents?
Replies: 6
Views: 415

Re: Hello and any pre-purchase 2-cents?

To add to PeteM's comment: With DIC set to maximum contrast, you most likely don't need darkfield. And a darkfield slider (which would barely work for 40x NA 0.65 objective) is nowhere as good as dedicated cardioid or paraboloid darkfield condenser (will will work for an 100x iris objective). You ma...