Search found 186 matches

by Tom Jones
Thu Jun 03, 2021 3:31 pm
Forum: Pictures and Videos
Topic: Actinosphaerium eichhorni
Replies: 9
Views: 407

Re: Actinosphaerium eichhorni

:shock: Damn that's beautiful!! :shock:
by Tom Jones
Wed May 05, 2021 2:33 am
Forum: Beginner's corner
Topic: eyepiece camera vs phone
Replies: 7
Views: 555

Re: eyepiece camera vs phone

Don, turn the phone horizontally if you crop in the phone, and the vertical format becomes horizontal. If you're shooting stills you can crop any way you want, horizontal, vertical, or rotated in some weird way to match specimen orientation (see the demo foraminifera shot cropped in Lightroom in the...
by Tom Jones
Mon May 03, 2021 7:27 pm
Forum: Beginner's corner
Topic: eyepiece camera vs phone
Replies: 7
Views: 555

Re: eyepiece camera vs phone

Cell phone cameras work just fine. If you're careful, and practice a bit, you can do very well hand-held. I've shot publication quality stills hand-held. You can do much, much better with a cell phone mount of some kind, even shooting very nice 4K or 8K video, if your phone will support it. I person...
by Tom Jones
Fri Mar 26, 2021 2:37 am
Forum: For forum members who want to buy and sell equipment
Topic: Ebay microscope sales scam. Anyone know the details?
Replies: 9
Views: 826

Re: Ebay microscope sales scam. Anyone know the details?

Talk about timing, here's the number of the fraudulent IX70 Ebay sale: 133704755178 I've reported this particular one, with other numbers I'm sure, several times. Just for sport, I'll refrain from reporting it for awhile so anyone can see what's going on, although his other item list come up as miss...
by Tom Jones
Fri Mar 26, 2021 1:47 am
Forum: For forum members who want to buy and sell equipment
Topic: Ebay microscope sales scam. Anyone know the details?
Replies: 9
Views: 826

Re: Ebay microscope sales scam. Anyone know the details?

I've seen a bunch of those over the last several months. IX-70/71 with a starting bid of $32 last time, or other absurd prices. They swipe listing pictures from legit listings, and sometimes copy the other data as well. Somewhere in the listing is often a photo of their "Buy it Now" really low price...
by Tom Jones
Mon Mar 22, 2021 11:22 pm
Forum: Pictures and Videos
Topic: Nikon Small world competition
Replies: 4
Views: 347

Re: Nikon Small world competition

That was done using an Olympus BHS, SPlan objectives and DIC. Video shot in 4K UHD using a Panasonic GH4.

Tom
by Tom Jones
Mon Mar 22, 2021 6:37 pm
Forum: Pictures and Videos
Topic: Nikon Small world competition
Replies: 4
Views: 347

Re: Nikon Small world competition

Yep. Honorable Mention 2018 Small World in Motion: https://www.nikonsmallworld.com/galleri ... er-feeding

Full video here: https://www.youtube.com/watch?v=mqZns4wo0AQ
by Tom Jones
Fri Mar 19, 2021 2:32 pm
Forum: Beginner's corner
Topic: Condensers, how do you properly use them?
Replies: 17
Views: 1365

Re: Condensers, how do you properly use them?

You might find this (long) video explanation, by Peter Evennett (Royal Microscopical Society and Quekett Microscopical Club) , of how microscope optics work useful: https://www.youtube.com/watch?v=60_jgZtyR6U It answers a lot of questions and I really wish I'd had it as a reference when I was first ...
by Tom Jones
Fri Mar 12, 2021 5:57 am
Forum: Microscopy accessories
Topic: Counting chambers
Replies: 9
Views: 775

Re: Counting chambers

And they certainly do have etched grids for counting. Counts wouldn't be reasonably possible otherwise.
by Tom Jones
Fri Mar 12, 2021 5:55 am
Forum: Microscopy accessories
Topic: Counting chambers
Replies: 9
Views: 775

Re: Counting chambers

It doesn't change anything. It's just a thick glass base designed to be washed and reused many, many times. For years if you're careful. The distance to the floor of the counting chamber from the top of the cover slip is well within the working distance of regular 40x objectives. That is what they w...
by Tom Jones
Fri Mar 12, 2021 1:38 am
Forum: Microscopy accessories
Topic: Counting chambers
Replies: 9
Views: 775

Re: Counting chambers

It's called a Hemocytometer. They provide a flat, gridded and highly accurate counting volume. Before automated RBC, WBC, and platelet cell counters these are what was used to perform the counts in clinical labs. Lots of sperm counts and body fluid (pleural, peritoneal and CSF) cell counts are still...
by Tom Jones
Thu Mar 04, 2021 4:24 pm
Forum: Microscopy accessories
Topic: Pol Sliders ... Advice needed please
Replies: 8
Views: 769

Re: Pol Sliders ... Advice needed please

MichaelG, That's indicating the "slow wave" axis for the waveplate. Slow waves and fast waves are properties of polarized light. You're going to find those interesting to play with. The sensitive tint should be a full wave (AKA first order) retarder, the quarter wave (lambda) retarder is just that, ...
by Tom Jones
Mon Feb 22, 2021 7:16 pm
Forum: Specimens, samples and slides
Topic: Amoebas dangerous ?
Replies: 13
Views: 2369

Re: Amoebas dangerous ?

I'm a retired Clinical Lab Scientist with a degree in clinical microbiology. I was a lab supervisor for 25 years. SB County Hospital, renamed Arrowhead Regional Medical Center when we moved to a new campus in 1999, was, and probably still is, the second busiest trauma center in California. As a resu...
by Tom Jones
Mon Feb 22, 2021 4:48 pm
Forum: Specimens, samples and slides
Topic: Amoebas dangerous ?
Replies: 13
Views: 2369

Re: Amoebas dangerous ?

To quote the CDC: Although most cases of primary amebic meningoencephalitis (PAM) caused by Naegleria fowleri infection in the United States have been fatal (144/148 in the U.S., 1), there have been five well-documented survivors in North America: one in the U.S. in 1978 2, 3, one in Mexico in 2003 ...
by Tom Jones
Tue Feb 02, 2021 4:50 pm
Forum: Identification help
Topic: Stool Microscopy - Parasite DD - Artifacts?
Replies: 6
Views: 646

Re: Stool Microscopy - Parasite DD - Artifacts?

Diagnostic parasitology isn't something you can learn quickly or easily. It takes good training, a lot of time at the microscope, and a whole lot of specimens, positive and negative, to even get adequate, so don't feel bad.
by Tom Jones
Tue Feb 02, 2021 4:12 pm
Forum: Identification help
Topic: Stool Microscopy - Parasite DD - Artifacts?
Replies: 6
Views: 646

Re: Stool Microscopy - Parasite DD - Artifacts?

It's been quite awhile since I did any diagnostic parasitology, but I think they were correct in not treating. The fact that you were left alone with the specimen should probably tell you something. I've no idea what the first two are, but they really don't look like eggs, or anything important to m...
by Tom Jones
Tue Feb 02, 2021 2:16 pm
Forum: Identification help
Topic: Stool Microscopy - Parasite DD - Artifacts?
Replies: 6
Views: 646

Re: Stool Microscopy - Parasite DD - Artifacts?

So, since you're in med school, what did your lab instructor say they were?
by Tom Jones
Thu Jan 14, 2021 4:35 pm
Forum: Pictures and Videos
Topic: Paratomy
Replies: 8
Views: 1479

Re: Paratomy

Aeolosoma hemprichi is very easy to keep in culture too. I kept some going for three years in a deep petri dish. Just feed them a little piece of spinach leaf once a week or so, and change the water when there gets to be too much debris left over. I've seen as many as four of them hooked together no...
by Tom Jones
Mon Jan 11, 2021 10:16 pm
Forum: Beginner's corner
Topic: Selecting BH2 adapter for photo AND video
Replies: 2
Views: 879

Re: Selecting BH2 adapter for photo AND video

Which Sony? Find out what part of the sensor is being used for the video resolution you intend to shoot. If it shoots video using the full frame at that resolution, your good with the NFK 2.5x. If it's smaller than full frame, you'll either need to accept the crop factor or come up with another solu...
by Tom Jones
Tue Jan 05, 2021 2:11 am
Forum: Microscopes and optics
Topic: Monochromatic Microscopes?
Replies: 14
Views: 1912

Re: Monochromatic Microscopes?

Color provides contrast among other things. Without color, you will need to find some other way to increase contrast enough to tell one thing from the other. It also provides more specific clues as to what you are looking at by way of differential staining. Think of the difficulty in distinguishing ...
by Tom Jones
Mon Jan 04, 2021 11:27 pm
Forum: Microscopes and optics
Topic: Monochromatic Microscopes?
Replies: 14
Views: 1912

Re: Monochromatic Microscopes?

I'm curious to know in what hematology applications field width is prized over all else, including color?
by Tom Jones
Mon Jan 04, 2021 5:16 am
Forum: Camera systems and imaging
Topic: Budget Friendly BH2 Video?
Replies: 40
Views: 5266

Re: Budget Friendly BH2 Video?

No, actually. I won't modify my photography systems to take pictures for you. And I have no inclination to check or comment on your math. I've explained what I use and why. Asking me to do testing for you so you can figure out why your math is confusing or misleading you is unreasonable, and not som...
by Tom Jones
Mon Jan 04, 2021 1:19 am
Forum: Microscopes and optics
Topic: Olympus eyepiece markings?
Replies: 3
Views: 817

Re: Olympus eyepiece markings?

The + inside a circle should mean there is a crosshair reticle in place. These are mostly used on polarizing microscopes and generally cost somewhat more than an eyepiece without the reticle as they are relatively rare. They are also handy if you are trying to collimate the eyepieces in a binocular ...
by Tom Jones
Sun Jan 03, 2021 9:59 pm
Forum: Camera systems and imaging
Topic: Budget Friendly BH2 Video?
Replies: 40
Views: 5266

Re: Budget Friendly BH2 Video?

The bottom line is that you need an image circle at the sensor that is large enough to enclose the entire area of the sensor that is used to capture the image. If it's smaller, you get vignetting. You really have very few reasonable choices as to how to get there. You are limited to the available pr...
by Tom Jones
Sat Jan 02, 2021 9:36 pm
Forum: For forum members who want to buy and sell equipment
Topic: Wanted to buy: Olympus U-DCW Oil Darkfield Condenser
Replies: 9
Views: 1292

Re: Wanted to buy: Olympus U-DCW Oil Darkfield Condenser

ScienceMatters - I just sent you a private message.

Tom
by Tom Jones
Sun Dec 27, 2020 3:27 pm
Forum: Camera systems and imaging
Topic: Budget Friendly BH2 Video?
Replies: 40
Views: 5266

Re: Budget Friendly BH2 Video?

I only use the GH4 for video on the microscopes, so I have no experience with it's electronic shutter in the photography mode.

Tom
by Tom Jones
Sun Dec 27, 2020 4:15 am
Forum: Camera systems and imaging
Topic: Budget Friendly BH2 Video?
Replies: 40
Views: 5266

Re: Budget Friendly BH2 Video?

A mag changer can help with photographic framing. You can actually do the same thing with different projection eyepiece magnifications too. I originally got one to help with framing specimens for outreach demonstrations, so the kids didn't have to search so much for what I was trying to show them. A...
by Tom Jones
Sun Dec 27, 2020 2:53 am
Forum: Camera systems and imaging
Topic: Budget Friendly BH2 Video?
Replies: 40
Views: 5266

Re: Budget Friendly BH2 Video?

I think using a calibration slide is the only way to get accurate dimensional information for scale bars. Basically, Photograph the calibration slide with the objectives you intend to use (or all of them to be complete) with the same camera and projection setup you will use with the specimens. If yo...