Search found 2546 matches

by MicroBob
Sun Feb 14, 2021 9:43 am
Forum: Specimens, samples and slides
Topic: Clean pond samples for DF imaging
Replies: 2
Views: 322

Re: Clean pond samples for DF imaging

Hi,
you might consiger a two step pipetting: Filter pond water, pipett organism from sample in vessel one, pipett it from there to vessel two.
Professionals sometimes use mouth operated pipettes for very selective pipetting.

Bob
by MicroBob
Sun Feb 14, 2021 7:20 am
Forum: Microscopes and optics
Topic: "New" eyepiece technology to eliminate "floaters"
Replies: 14
Views: 602

Re: "New" eyepiece technology to eliminate "floaters"

Hi Pete, interesting product, thank you for pointing out! Maybe Isis is going to hynotize you with the spinning discs! There used to be a Zeiss West projection screen "boob" that included a spinning or oszillating frosted screen in the later models. Moving the screen reduced the gritty effect of the...
by MicroBob
Sat Feb 13, 2021 7:58 pm
Forum: Pictures and Videos
Topic: Centric diatom from Wakura Beds, Japan or how quick do diatoms travel?
Replies: 31
Views: 1691

Re: Centric diatom from Wakura Beds, Japan or how quick do diatoms travel?

it should not be very sensitive to the coverslip thickness. Hi Doron, I would think so too. I really don't have an idea what the difference might be. What I observed was that some of my objectives did better than others but the simple 100:1 achromat did well. Perhaps it really is related to the dia...
by MicroBob
Sat Feb 13, 2021 8:17 am
Forum: Specimens, samples and slides
Topic: Colourful Exploration of Lichen Structure
Replies: 12
Views: 1079

Re: Colourful Exploration of Lichen Structure

Hi John, very interesting observations! It really is fascinating that fungus and algae find togehter to form a cooperation - something we humans often don't manage though we have hands and a brain. And this not only under an overhang inside a crater somewhere but in many and different places. Your p...
by MicroBob
Sat Feb 13, 2021 6:47 am
Forum: Pictures and Videos
Topic: Snow flake photography - post your pictures!
Replies: 19
Views: 1365

Re: Snow flake photography - post your pictures!

Great set up! With it you work very much like Anthony von Leeuwenhoek only in a different magnification range.
by MicroBob
Fri Feb 12, 2021 7:10 am
Forum: Illumination Techniques
Topic: Made my own laser cut darkfield & oblique filters
Replies: 9
Views: 1038

Re: Made my own laser cut darkfield & oblique filters

Hi, your laser cut filters or stops look great! Compared to milk carton cut outs they also don't get lost so easily. A good base for these stops are round cover slips that are available in sizes of e.g. 32mm. The best contrast/resolution combination will probably be available form circular oblique l...
by MicroBob
Fri Feb 12, 2021 6:53 am
Forum: Beginner's corner
Topic: No tool required?
Replies: 9
Views: 668

Re: No tool required?

Does it look similar to the rings that hold the glass in camera filters? These are usually screwed in as Hans has described. I somtimes clamp slot screw bits in a machinist vise in the right distance and set the part onto them from above. Before you start working you might put a disc of cardboad or ...
by MicroBob
Thu Feb 11, 2021 8:58 pm
Forum: Camera systems and imaging
Topic: $15 well spent
Replies: 2
Views: 629

Re: $15 well spent

Hi, I have the same Swift holder, my son has the second, and I can overall recommend it. It works best if one uses with a separate eyepiece that stays in the holder. The sleeve is hollow, so it can't be turned out on the lathe, but it is from polystyrene that softens at fairly low temperatures and c...
by MicroBob
Thu Feb 11, 2021 8:40 pm
Forum: Microscopy accessories
Topic: Cleanliness of Zeiss 1.5H cover slips
Replies: 21
Views: 1018

Re: Cleanliness of Zeiss 1.5H cover slips

I once had a closer look at a lot of slides and cover slips of very different origin and age. I found most cover slips to be in usable condition but of cause of different states of cleanliness. With the slides it is very different: Here sometimes two year old packages already had a decayed surface t...
by MicroBob
Thu Feb 11, 2021 4:24 pm
Forum: Microscopy accessories
Topic: Cleanliness of Zeiss 1.5H cover slips
Replies: 21
Views: 1018

Re: Cleanliness of Zeiss 1.5H cover slips

Good enough for my needs and available time. Probably not good enough for yours though.
by MicroBob
Thu Feb 11, 2021 4:00 pm
Forum: Specimens, samples and slides
Topic: Lichen Slide Making From Scratch
Replies: 21
Views: 1253

Re: Lichen Slide Making From Scratch

Hi John, good that you had an alternative in this situation! Most likely it was just an aged condensator that blew: https://www.digikey.com/catalog/en/partgroup/pme271m-series/4042 and all else could be ok. So if you had somebody with real electrics knowledge it could be a worthwhile repair even if ...
by MicroBob
Thu Feb 11, 2021 3:48 pm
Forum: Microscopy accessories
Topic: Cleanliness of Zeiss 1.5H cover slips
Replies: 21
Views: 1018

Re: Cleanliness of Zeiss 1.5H cover slips

Hi Viktor, for cleaning lenses I use "Wundbenzin", medical spirit, which is similar to lighter fluid but cleaner. For fairly dirty new cover slips I took a 53ml marmelade glass, threw them all in and filled op with isopropanol. If need one i fish it out with forceps and clean it with papaer tissue a...
by MicroBob
Thu Feb 11, 2021 7:01 am
Forum: Pictures and Videos
Topic: Centric diatom from Wakura Beds, Japan or how quick do diatoms travel?
Replies: 31
Views: 1691

Re: Centric diatom from Wakura Beds, Japan or how quick do diatoms travel?

Hi together, these images raised an interesting question: Hobbyst46-Doron didn't quite get these results though he is using a first class instrument and a slide that should be virtually identical. What is the difference? Doron made both slides so the number of possible differences is limited. Here s...
by MicroBob
Wed Feb 10, 2021 5:56 pm
Forum: Beginner's corner
Topic: What Microscope Is Needed To See Attached Images
Replies: 6
Views: 350

Re: What Microscope Is Needed To See Attached Images

Hi,
the last imaged specimen is stained, the second last was probably made with a microscope with DIC, the first image could be had with about any microscope.

Bob
by MicroBob
Wed Feb 10, 2021 5:49 pm
Forum: My microscope
Topic: My old black Cogit
Replies: 8
Views: 422

Re: My old black Cogit

Nice microscope setup, Joël!
These horse shoe microscopes are not often used today which is a pity as they perform very well, even for photography. Your setup for the tablet is very ingenious and a good idea to copy for people who can't look through eyepieces very well.

Bob
by MicroBob
Wed Feb 10, 2021 5:43 pm
Forum: Specimens, samples and slides
Topic: Lichen Slide Making From Scratch
Replies: 21
Views: 1253

Re: Lichen Slide Making From Scratch

Hi John,
these bigger cassettes look to be very usefull. I have just the small ones and use them as the base of the block. For bigger specimen I have two tea eggs. Especially nice is the one without paper clips as it fits in a fairly small round glass.

Bob
by MicroBob
Wed Feb 10, 2021 5:31 pm
Forum: Microscopy accessories
Topic: Cleanliness of Zeiss 1.5H cover slips
Replies: 21
Views: 1018

Re: Cleanliness of Zeiss 1.5H cover slips

Hi Viktor, I also bought Zeiss cover slips from an ebay seller. He said they were included with Zeiss Objectives and not needed wich I don't doubt. They have a very even thickness of slightly over 0,17mm, ca. 0,175, within tolerance but probably near the upper limit. A micrometer is not really speci...
by MicroBob
Wed Feb 10, 2021 3:54 pm
Forum: Introduce yourself
Topic: Retired chemist, hobby brewer, near Paris F.
Replies: 4
Views: 328

Re: Retired chemist, hobby brewer, near Paris F.

Hi Joël, welcome to the forum! Chemistry is valuable knowledge for a microscopist, every prepatarion method needing another bottle of something.... You will probably find a lot of information on micro technique that will be useful for you in this forum. Can you post some examples of what a beer brew...
by MicroBob
Wed Feb 10, 2021 2:22 pm
Forum: Specimens, samples and slides
Topic: Lichen Slide Making From Scratch
Replies: 21
Views: 1253

Re: Lichen Slide Making From Scratch

Hi John, so far I have moved my plant parts individually with forceps, and often they were a bit too big for the cassettes. But these fine lichen bits really make the cassettes indispensable! I'm looking forward to see the next steps of your process. There are fairly few amateurs who do paraffin emb...
by MicroBob
Tue Feb 09, 2021 5:03 pm
Forum: Pictures and Videos
Topic: Snow flake photography - post your pictures!
Replies: 19
Views: 1365

Re: Snow flake photography - post your pictures!

Hi together,
I took a photo of the improvised setup.
Next to the fresh crystals I also tried to make permanent slides. Crystals trapped in a hard UV curing finger nail seal look very good when still cold. A layer of snow in LOCA is too much for a good image.

Bob
by MicroBob
Tue Feb 09, 2021 12:04 pm
Forum: Pictures and Videos
Topic: Snow flake photography - post your pictures!
Replies: 19
Views: 1365

Re: Snow flake photography - post your pictures!

Hi together,
here a couple of shots of fresh snow crystals from this morning. Taken with the Swift FM-31 inverted field microscope, side illumination with batty powered work light, improvised dark field, exposure ca. 1 sec.

Bob
by MicroBob
Mon Feb 08, 2021 9:21 pm
Forum: Pictures and Videos
Topic: Centric diatom from Wakura Beds, Japan or how quick do diatoms travel?
Replies: 31
Views: 1691

Re: Centric diatom from Wakura Beds, Japan or how quick do diatoms travel?

Nice design and much quicker to build! In old times they would have used a bulb to heat the thing - try that with todays LEDs! :lol: Your room temperature will probably drop by another degree but you could increase the surface temperature by applying a layer of Armaflex insulation to the underside. ...
by MicroBob
Mon Feb 08, 2021 8:39 pm
Forum: Pictures and Videos
Topic: Centric diatom from Wakura Beds, Japan or how quick do diatoms travel?
Replies: 31
Views: 1691

Re: Centric diatom from Wakura Beds, Japan or how quick do diatoms travel?

Hi Louise, good idea, the panel mount resistor. What increased the build time of my micro hot plate was the PID controller with additional wiring, milling, thread cutting... Recently I made lots of botanic slides for a test series for a new stain. My usual hot plate was too small and got on my nerve...
by MicroBob
Mon Feb 08, 2021 7:13 pm
Forum: Pictures and Videos
Topic: Centric diatom from Wakura Beds, Japan or how quick do diatoms travel?
Replies: 31
Views: 1691

Re: Centric diatom from Wakura Beds, Japan or how quick do diatoms travel?

2. Could be that Pleurax was not fully cured. The Pleurax protocol from the site diatoms.nl states that heating the slide for 15 min at a temperature of 180C is sufficient. However, I have often observed that the resin was not fully cured. Remained slightly tacky. So did not solidify. How can we so...
by MicroBob
Mon Feb 08, 2021 7:01 pm
Forum: Specimens, samples and slides
Topic: Lichen Slide Making From Scratch
Replies: 21
Views: 1253

Re: Lichen Slide Making From Scratch

Hi John,
nice series! It is obvious why you needed a new stereo microscope - the old one had a smaller foot! :lol:

Bob
by MicroBob
Mon Feb 08, 2021 9:58 am
Forum: Pictures and Videos
Topic: Centric diatom from Wakura Beds, Japan or how quick do diatoms travel?
Replies: 31
Views: 1691

Re: Centric diatom from Wakura Beds, Japan or how quick do diatoms travel?

Hi together, I did some more technical tests: -The delaminated 63 Planapo really doesn't resolve as well as the NPN Fluotar 100 -With phase contrast I didn't get a good image of the fine structure. Perhaps the halos superimpose and cover fine detail -Bright field best showed the real structure -The ...
by MicroBob
Mon Feb 08, 2021 9:42 am
Forum: Pictures and Videos
Topic: Broom sections in different stains
Replies: 9
Views: 904

Re: Broom sections in different stains

Hi, the microtome is an old Sartorius hand/cylinder microtome of good quality. I only made the holder for it. What I designed was the 3D-printed blade holder that makes sectioning fairly easy and repeatable. Recently I have cut material embedded into PEG to nice even 20µ sections with this setup. I'...
by MicroBob
Mon Feb 08, 2021 6:58 am
Forum: Specimens, samples and slides
Topic: Lichen Slide Making From Scratch
Replies: 21
Views: 1253

Re: Lichen Slide Making From Scratch

Hi John, I'm following your project with great interest! For the cleaning of the lichen I had an idea: A proven method to remove fresh spots from carpets is to soak them with carbonated water (right word?). The bubbling apparently loosens dirt and makes it easy to remove it. This might be just the r...
by MicroBob
Mon Feb 08, 2021 6:53 am
Forum: Pictures and Videos
Topic: Snow flake photography - post your pictures!
Replies: 19
Views: 1365

Re: Snow flake photography - post your pictures!

Hi, good idea, this should make it possible to quickly take some shots without becoming really cold from standing around in th storm. Some cameras even have tiltable displays so one could mount the setup onto a tripod. Yesterday I have attatched some slides and some cover slips to blocks of wood (st...
by MicroBob
Sun Feb 07, 2021 4:39 pm
Forum: Pictures and Videos
Topic: Snow flake photography - post your pictures!
Replies: 19
Views: 1365

Re: Snow flake photography - post your pictures!

Hi Fan, I never did that myself so far but sit here prepared. Only problem is that the show fall border is somewhat south of here and only moves slowly, but it might arrive here. I found one description of method B here: http://www.snowcrystals.com/preserve/preserve.html But I don't find this method...