MicrobeHunter.com Microscopy Forum

Nothing's working when trying to upload images within a post. It seems to upload, then I keep getting:

"The image file you tried to attach is invalid."

Anyone else seen or is getting this error?

Where's it going?

What's it thinking?

Does it dream?

Does it hope?

Does it know of us?



...so many questions.

A beer says less than 2 fifty.

I suspect the scale bar wasn't recorded when it was scanned. Aesthetics or something.

What is the field of view on these images?

I made one for pennies using a doorknob protector and a piece of PVC.




And some double-sided tape.

I can't imagine what problem a frosted plate solves that a simple sheet of vellum or parchment paper couldn't solve for far cheaper.

Also, a clear plate underlain with un-illuminated black paper makes nice darkfield stereoscopy possible. Absolutely go with a clear plate.

I agree, it's worth a try. However....

20x and above M-type objectives won't like the cover glass. On the other hand, if you're using standard objectives for cover glass, you might be limited by depth of field above 20x.

stjepo wrote: ↑

Tue Nov 14, 2023 9:11 am

With patience and a lot of time I have restored a Nikon SMZ-10. I have polished one of the lenses that were scratched, cleaned the mould on the lenses....

Not to hijack this awesome thread, but what product and procedure did you use to polish your lenses?

Thanks, in advance!

Hola Jose....do you have a sample DIC microscopy image with that condenser side 20x DIC prism AND 20x objective with its DIC prism engaged? I'm considering a condenser modification like yours for my Optiphot, but I'd like to see if its' better than my Sanderson plate. I suspect it is, but it would b...

Where does this shading come from? Is it simply the obliqueness of the lighting source? Jose, can you post your DIC images in full color? I'd be curious to see if you have any rainbow artifacts. That might answer apo's question. I'm taking Jose's claim for DIC at face value given that he's using a ...

Hey, that's pretty good!

What you are doing suddenly comes into "focus"!

He sells them as "loose' pieces, correct?

I think he removes them from old PZO condensers. I have looked at those too.

...still following.


What is your plan for the condenser DIC Prism?

Macro_Cosmos wrote: ↑

Mon Nov 27, 2023 1:22 pm

Converting a single LED for autofluorescence is pretty simple, find a cheap aspherical lens and collimate the LED. You can lift .step files from ThorLabs' website and design a housing to mount the thing onto your microscope arm.

Nice.

Thanks!

Your "diagnostic test slide" shows promise, I think. However, to be truly diagnostic you'd probably want to know the particle size and use a mountant as close to a RI of 1.5 as possible. Maybe we could, as a community, find "inks" with a particle size in the 300nm range; diagnosing super-resolution ...

I have a few questions. Is this autofluorescence or did you use any dyes? If they were dyes, what kind and how did you prepare the solution? And finally, how did you induce the fluorescence effect (UV or blue)? I too, have these questions, plus: Are you using UV-specific objectives, slides , and mo...

MichaelG. wrote: ↑

Wed Mar 17, 2021 7:19 pm

... Now I will have no hope of getting anything done !

same.

I do not have a phase telescope, though it would be nice to have. I don't think I've ever seen one come up on ebay. Would a phase telescope be a useful substitute for a Bertrand-Amici lens? ....and no, I don't have a slide ringing table. Putting that much effort into a slide that may have mountant p...

Thanks apo.

Yep, there's a tiny bit of abberation that I removed in post.

I may yet fine tune this prototype by finding the focal point of the Abbe condenser lens and setting the immersion ball-bearing lens there. The problem is that it's already so good that I'd hate to screw it up.

Success!.....(I think) Before selling the other kidney on a dedicated Nikon oil immersion condenser, I took a few spare parts and cobbled together a working franken-condenser: Screenshot 2023-11-22 171208.jpg I took the head off an old Leitz immersion condenser head and slapped it on a useless Nikon...

Scale bars, good sir.....just pretty pictures without 'em.

The vast majority of my observations are autofluorescence, i.e. the ability of preparations to spontaneously "glow" under the influence of excitation lighting. But I would like to go a step further and start using fluorescent dyes such as acridine orange, fluorescein, rhodamine. The dyeing techniqu...

Was that ground glass useless as a viewing option? Was it not useful for showing others what you were looking at?

Scoper wrote: ↑

Sat Oct 14, 2023 10:46 pm

Thks for responding..anyone else?

Thks

a good microscope

Basically Sanderson prisms without the external stress frame, pretty much any method works as long the correct stress gradient is created in the polymer. I am not familiar with frameless stress gradient prisms. How do they work? Having built my own Sanderson plate, I can't see how the system could ...

Honestly, once you leave the eyepiece and start imaging for playback on various devices, magnification is essentially meaningless. You would do better getting a micrometer slide, taking photos of it with your different objectives, and calculating your field of view. Adding scale bars based on your ...

I like that you took the time to embed the video and not just post a link.

Thumbs up!!

daruosha wrote: ↑

Thu Feb 27, 2020 1:36 pm

"journey to the microcosmos" videos are really nice but the narration over the videos really turns me off, not that they are unscientific, but the way narrator speaks, gives me heebie-jeebies. I cannot stand his tone of voice.

That's funny....and I thought it was just me.

Wow! That's awesome diversity! On Long Island no less!

My plankton drag in Puget Sound last year yielded far fewer varieties!