Search found 353 matches
- Fri May 01, 2020 10:41 pm
- Forum: Beginner's corner
- Topic: Specimen ethics
- Replies: 42
- Views: 17909
Re: Specimen ethics
A big reason why isopropyl alcohol should be kept in a tightly closed container is that it is very hygroscopic, at least until it dilutes itself down to 65% (35% water).
- Tue Apr 28, 2020 7:40 am
- Forum: Beginner's corner
- Topic: Need advice buying a Reichert Microstar IV
- Replies: 23
- Views: 9252
Re: Need advice buying a Reichert Microstar IV
75RR, I agree with everything you have laid out about ebay descriptive terms. However, I do think that necessarily ascribing nasty motives to a vendor (i.e., 'playing' you) is often inaccurate. Many of these mass vendors of used equipment may just be covering themselves against over-representing the...
- Tue Apr 28, 2020 3:38 am
- Forum: Beginner's corner
- Topic: Need advice buying a Reichert Microstar IV
- Replies: 23
- Views: 9252
Re: Need advice buying a Reichert Microstar IV
For the neophytes reading the above, I second Apo's analysis of ebay returns. I have had very good luck with 'no returns' in getting a refund and/or free ship-back. In many cases, the seller gave a refund and said 'just keep it, no problem', not wanting to go through the headache of putting it back ...
- Fri Apr 24, 2020 11:44 pm
- Forum: Beginner's corner
- Topic: Specimen ethics
- Replies: 42
- Views: 17909
Re: Specimen ethics
Perhaps part of the cognitive disconnect that raises questions like this, i.e., I feel sorry for my microbes on my slide, I hate to hurt them, is it ok to flush them, etc., etc., arises from the mere fact that when examined under the microscope, the apparent size of the microlife is very macro, and ...
- Sat Apr 18, 2020 6:12 pm
- Forum: Beginner's corner
- Topic: Need advice buying a Reichert Microstar IV
- Replies: 23
- Views: 9252
Re: Need advice buying a Reichert Microstar IV
A great clinical/lab microscope, of an era between the older heavy brass-and-steel beauties, and the modern stylish-but-plastic Chinese-made types. Some of the best planachro optics out there. Look for one with: 1) full complement of objectives, and a couple of eyepieces, 2) smooth focusing througho...
- Fri Apr 17, 2020 12:25 am
- Forum: Microscopes and optics
- Topic: objectives labeled DIC
- Replies: 2
- Views: 1455
Re: objectives labeled DIC
1. No. Yes. 2. No. 3. It is labeled DIC because it is a strain-free, i.e., won't exhibit any colors when examined with polarized light; obviously that would interfere with DIC or polarization effects when using those techniques. Strain-free was originally required for polarization techniques, e.g., ...
- Wed Apr 15, 2020 5:40 pm
- Forum: Camera systems and imaging
- Topic: Sony APS-C on Swift Trinocular
- Replies: 7
- Views: 3544
Re: Sony APS-C on Swift Trinocular
+1, Apo. Well stated.
- Sun Apr 12, 2020 6:41 pm
- Forum: Introduce yourself
- Topic: Adding / requiring member locations?
- Replies: 15
- Views: 5909
Re: Adding / requiring member locations?
I certainly can appreciate both perspectives: the desire for some anonymity on the part of the poster, yet the frustration of readers who wonder "where the heck are you posting from?" Many posters, especially neophytes who are asking for advice on a possible purchase, are strongly encouraged to add ...
- Sun Mar 22, 2020 12:06 am
- Forum: Camera systems and imaging
- Topic: Olympus BHS vs modern microscope
- Replies: 9
- Views: 6943
Re: Olympus BHS vs modern microscope
Most comparisons, very much such as the one you linked to, are specious, and are generally poorly done. It is most always an apples-to-oranges comparison: the N.A.s are not the same; the focus is on different points of the specimen for the different lenses; the magnifications are not the same; the p...
- Fri Feb 28, 2020 4:46 pm
- Forum: Microscopes and optics
- Topic: Leica Reichert Jung Polyvar (models)
- Replies: 88
- Views: 37272
Re: Leica Reichert Jung Polyvar (models)
Member wporter is in the midst of a move and does not have access to documentation, nor his Reichert objectives, so he's not much help. My scope is a Univar, but uses some of the same optics as the Polyvar Met. But I will say that the Plan Fluors go down at least to 5x, although quite frankly, at th...
- Mon Feb 10, 2020 5:54 pm
- Forum: Beginner's corner
- Topic: DNA?
- Replies: 7
- Views: 6122
Re: DNA?
DNA is visible with a 100x objective, but it is all coiled up (with the coils coiled, etc.) into a chromosome. So you will not be able to see the actual double-helix of DNA, just the giant hyper-organized wad that is the chromosome: a reduction in length from the meter(s)-long DNA strand into a chro...
- Mon Feb 10, 2020 5:35 pm
- Forum: Microscopes and optics
- Topic: B&L (or Cambridge) PhotoZoom inverted microscope?
- Replies: 5
- Views: 5832
Re: B&L (or Cambridge) PhotoZoom inverted microscope?
The ornamental design is downloadable from here (USD308879, 1990): http://www.freepatentsonline.com/D308879.html The rubber objective turret cover patent (!) along with a lot more (!?), is detailed here (US 4,770,520, 1988): http://www.freepatentsonline.com/4770520.html Why the patent 4,770,520 has ...
- Mon Feb 10, 2020 12:08 am
- Forum: Microscopes and optics
- Topic: 23.2 vs 30mm
- Replies: 38
- Views: 19036
Re: 23.2 vs 30mm
"I have usually heard it described as the FoV you'd get at 1x..." Yes, that's right; thanks, Scarodactyl. It is still a cumbersome number. So you have a real FOV with a 60x objective, e.g., that in microns will be (FOVmm*1000)/60). Fine; you want to measure a critter's length for a scale bar on the ...
- Sun Feb 09, 2020 5:39 pm
- Forum: Microscopes and optics
- Topic: 23.2 vs 30mm
- Replies: 38
- Views: 19036
Re: 23.2 vs 30mm
Well said, Phil. Nice summary. I, too, would prefer standardization on apparent field. FOV in mm seems somehow extracted from some sort of optical design calculations, with not much users can relate to. It would be nice to have an easy way to determine apparent field of an eyepiece (relative field s...
- Thu Jan 30, 2020 2:16 am
- Forum: Microscopes and optics
- Topic: Leica Reichert Jung Polyvar (models)
- Replies: 88
- Views: 37272
Re: Leica Reichert Jung Polyvar (models)
"I'm wondering if it would be possible to put together a best guess on the attributes of various AO/Reichert/Leica infinity optics?" What a great idea. Maybe also separate columns for thread size (e.g., M28 for some of the Reichert Austria ones for the Univar/Polyvars), and for the needed type of ey...
- Thu Jan 30, 2020 2:08 am
- Forum: My microscope
- Topic: Reichert Polyvar Microscope
- Replies: 60
- Views: 30755
Re: Reichert Polyvar Microscope
It's a one-part, UV-hardening liquid. Relative to epoxies or other messy or problematic adhesives like super glues, very nice stuff to work with. For those who are using it to repair joined prism assemblies, stacked lenses, or other complex arrangements, some kind of jig or other hold-it-all-in-plac...
- Wed Jan 29, 2020 8:11 pm
- Forum: My microscope
- Topic: Reichert Polyvar Microscope
- Replies: 60
- Views: 30755
Re: Reichert Polyvar Microscope
"Do you have to put the resin in a vacuum to remove possible bubbles?" No, definitely not. Although this depends on not having any entrained bubbles to begin with. Bubbles will arise from shaking the Norland bottle before using, or from applying in a manner that adds bubbles as you apply a drop to o...
- Wed Jan 29, 2020 5:42 pm
- Forum: My microscope
- Topic: Reichert Polyvar Microscope
- Replies: 60
- Views: 30755
Re: Reichert Polyvar Microscope
For curing Norland 61, I use a UV flashlight for the initial set, at a few different angles for 20 min-1 hour total exposure. I then lay it out in the sun for a couple of days, usually placing the lens or prism on top of a mirror or white cardboard. Never had any failures, knock on wood.
- Sun Jan 26, 2020 7:57 pm
- Forum: Beginner's corner
- Topic: What affects the image produced from two different microscopes?
- Replies: 5
- Views: 3594
Re: What affects the image produced from two different microscopes?
You've sorta answered your own question.... Not to harsh on the inquiry, because it's a good one, and the images are good, too, but you must decrease the number of variables. That is, image the same subject, at the same magnification, polarizer angle, lighting (as much as possible), etc. You cannot ...
- Thu Jan 23, 2020 12:42 am
- Forum: My microscope
- Topic: Help on Leitz lens...can you help me with the shopping list ?
- Replies: 8
- Views: 6927
Re: Help on Leitz lens...can you help me with the shopping list ?
The EFs are the lowest grade of objective, I believe achromats. You might want to purchase only the NPL (near-plan?) or the better PL (wider zone of planarity than NPL).
- Wed Jan 15, 2020 5:07 pm
- Forum: Beginner's corner
- Topic: Advice on next upgrade to a T390, looking to observe yeast and bacteria.
- Replies: 10
- Views: 4935
Re: Advice on next upgrade to a T390, looking to observe yeast and bacteria.
Really well-done exposition and images, Phil. Very nice comparisons.
I wish we had more of this kind of answer on this forum; good images of this or that to support a point are generally worth much more than mere verbal descriptions.
I wish we had more of this kind of answer on this forum; good images of this or that to support a point are generally worth much more than mere verbal descriptions.
- Fri Jan 03, 2020 1:23 am
- Forum: Pictures and Videos
- Topic: Salt mix
- Replies: 18
- Views: 9094
Re: Salt mix
Very dramatic, and nicely focused.
- Tue Dec 24, 2019 11:44 pm
- Forum: For forum members who want to buy and sell equipment
- Topic: Olympus BH2 BHS UMA Nomarski DIC Metallurgical Microscope
- Replies: 6
- Views: 4005
Re: Olympus BH2 BHS UMA Nomarski DIC Metallurgical Microscope
You might get a manual for this Zeiss and become familiar with the DIC components that are necessary, and then talk to the seller to make sure all of the components come with the scope. Oftimes sellers claim DIC, but half or all of the needed parts are defective or missing. It is highly desirable th...
- Mon Dec 23, 2019 6:26 pm
- Forum: Beginner's corner
- Topic: Does a DSLR adapter need a lens in it?
- Replies: 38
- Views: 14250
Re: Does a DSLR adapter need a lens in it?
Viewing eyepieces will also project a real image, and are not that different from projection eyepieces in that regard. This is easily confirmed by holding a ground glass screen or frosted slide above your normal (viewing) eyepiece; you will get a real image projected upon the screen. The real and vi...
- Mon Dec 23, 2019 4:58 pm
- Forum: Beginner's corner
- Topic: Does a DSLR adapter need a lens in it?
- Replies: 38
- Views: 14250
- Fri Dec 20, 2019 12:39 am
- Forum: Illumination Techniques
- Topic: dIY DIC
- Replies: 68
- Views: 39870
Re: dIY DIC
The objectives marked 'DIC' don't have internal prisms, but rather have high-quality strain-free & occlusion-free optical glass. There is still a need for a prism external to and above the objective, and one below the objective. See the below page for a nice color graphic (Fig. 1) of a DIC optical s...
- Wed Dec 18, 2019 11:18 pm
- Forum: Microscopes and optics
- Topic: Urgent! Labovert Leitz - please advise!
- Replies: 12
- Views: 5934
Re: Urgent! Labovert Leitz - please advise!
I don't need any of these parts at the moment; too much duplication of what I have already. Thanks for the heads-up, though.
Anyone else? Even though it's on Facebook Marketplace (which I guess is like Craigslist), the seller may package it up and ship it for a small sum.
Anyone else? Even though it's on Facebook Marketplace (which I guess is like Craigslist), the seller may package it up and ship it for a small sum.
- Wed Dec 18, 2019 2:59 am
- Forum: Microscopes and optics
- Topic: Urgent! Labovert Leitz - please advise!
- Replies: 12
- Views: 5934
Re: Urgent! Labovert Leitz - please advise!
It might work; it has to fit properly. The Leitz stage for this scope looks just like an upright scope x-y stage, and in fact may be one.
- Tue Dec 17, 2019 10:01 pm
- Forum: Specimens, samples and slides
- Topic: Help to identify anomaly in blood.
- Replies: 7
- Views: 5031
Re: Help to identify anomaly in blood.
I'm sorry, but we're not allowed to give medical diagnoses on this forum.
But it was nice knowing you, MicroBob.
But it was nice knowing you, MicroBob.
- Tue Dec 17, 2019 7:27 pm
- Forum: Specimens, samples and slides
- Topic: Help to identify anomaly in blood.
- Replies: 7
- Views: 5031
Re: Help to identify anomaly in blood.
Possibly an air bubble.