MicrobeHunter.com Microscopy Forum

Apo, you are right the 100* adds to the experience. But, in my personal experience when using the 100* oil objective on pond sample slides, the coverglass often moves when scanning the slide. And when using the 100* I often want to switch back to a lower enlargement, requiring a 40* or 50* oil, (an...

Apo, you are right the 100* adds to the experience. But, in my personal experience when using the 100* oil objective on pond sample slides, the coverglass often moves when scanning the slide. And when using the 100* I often want to switch back to a lower enlargement, requiring a 40* or 50* oil, (an...

Great looking ciliate and very nice imaging.

Thanks, I'll take a look.

Thank you for sharing your experience, Harry. I forgot to mention that I have no filter running, on purpose. My bubbler / air pump looks a little too powerful for my 3.5 gallon tank, to my untrained eyes, even though it is the smallest air pump (“up to 10 gallons”) I could find. You can get a cheap...

Javier wrote: ↑

Sat Dec 09, 2023 2:51 pm

Wonderful!

Thanks

Macro_Cosmos wrote: ↑

Sat Dec 09, 2023 1:44 pm

16x, 32x, 64x and 120x.
No scales and I will not use them again, I like dynamic zoom.

Very nice imaging!

Image 1: Ascorbic Acid (Vitamin C) melted and cooled to form crystals. Imaged using polarized light. FOV: 0.71 x 0.40 mm. Image 2: Ascorbic Acid and Urea melted and cooled to form crystals. Imaged using polarized light. 4 panel panorama (shot using same objective as image 1). ascorbic-acid-1-high-re...

Perhaps Euplotes

Wonderful set of images

Leitzcycler wrote: ↑

Thu Nov 23, 2023 3:13 pm

Yes, It would be nice to have anyway. Please, send it.

PM sent

I have a PDF of exploded diagrams, not sure it would be helpful but if you want it I can send a link to you.

You just loose calibration forever. OSB does not have any measurement feature. Are you referring to measuring the size of the organism? Create your own scale bar for each objective you use (using a micrometer slide), add them as overlays in OBS, and you'll never need to be locked into any one softw...

Horse. Squid. Chicken. Tomahawk. Swiss Army Knife.

einman wrote: ↑

Sat Nov 18, 2023 2:33 am

Nice website!
https://www.thaimicrocosmos.com/tag/oil-immersion/

Thanks! It's due an update with some recent videos. Maybe this weekend.

Glad to hear your'e back in the game! I hope you find more and more

Some of my favorites from the first two weeks making and imaging crystals.

I have so many plants, maybe not. But at the start there were fish (still are) and shrimp. I had the bubbler so didn't see a reason not to use it.

Very nice.

So beautiful, I love the second one! Me too, it's now my desktop wallpaper :-) I regret that I only shot it in 1080P video for stacking, not at the full resolution of my camera with individual frames. And I've since remelted the slide so I can't take it again. Lesson learned. It would have made a r...

This past week I've begun playing around dissolving and melting various chemicals/substances, then imaging the resulting crystal formations using polarized light. It's an absolutely fascinating new area of the hobby for me. I began with some substances I had around the house including crushed Tyleno...

Today I got the fluorescence system working with my Nikon inverted diaphot. Here are a couple of short video clips: First, pondwater stained with Acridine Orange, which emits green light through a FITC cube: cXcRNphpJuk Second, pondwater autofluorescence using a red emission TRIITC cube: 2sDWhpW1YJU...

Brightfield only with the polarized light? Why the waveplate? Thanks for posting Yes this is brightfield. The order of pieces: Lamp Diffuser Polarizing film Wave plate Slide Polarizing film Objective Camera The wave plate adds color into the background and otherwise non-birefringent portions of the...

Here are a few scenes from my Nikon inverted TMD Diaphot. 2 pieces of linear polarizing film and a full lambda wave plate.

Wonderful video.

VaVaVaVoom?

The Lumix GH4 has HDMI out so you can connect it to an external monitor for live view without the need for a computer or software. You can then record internally. I can't comment on connectivity with a computer and software for viewing and recording, but even if you can with this camera, you would w...

Nice imaging, almost like x-rays

Thanks!

Nice setup and results. I have the same camera and lens. Can you link to the adapters used to step down to the objective?