MicrobeHunter.com Microscopy Forum

I have a Nikon TMD Diaphot with an ELWD 0.3 phase contrast condenser. From a physics/optics standpoint, is there any reason a higher NA condenser wouldn't work with this scope? I don't use petri dishes so I don't need the working distance. I'm wondering if there is a Nikon condenser with higher NA t...

Wonderful imaging

I've had a 20L pondwater microorganism aquarium going for about a year now. I've probably posted a bit about it in my own thread here, but when I have some time I'll post images and descriptions of the adventure in this thread so it's consolidated.

Bruce Taylor wrote: ↑

Tue Jan 30, 2024 12:52 pm

macnmotion wrote: ↑

Tue Jan 30, 2024 9:12 am

Do you get to name this species?

Only if our paper is accepted for publication.

You'll have to let us know. I'd love to read the paper when it's published.

Bruce Taylor wrote: ↑

Tue Jan 23, 2024 1:15 pm

This one is from a new (undescribed) species from a local bog:

Do you get to name this species?

You've just pretty much ruined any future imaging I'll do of tetate amoeba

Incredible set of images.

I've just acquired a Leitz Diavert microscope and am just about to make slides appropriate for the viewing requirements. I intend cutting 12 mm holes in standard slides and gluing a cover slip in place. Has anyone done this? Is there anything I should beware of other than ensuring a good seal all r...

Very cool. I usually don't see so many together unless it's an attached colony (e.g. Sinantherina socialis).

Wonderful!

Pond snails have statocysts

Very nice video. It's always hard zoomed in like that to follow these quick ciliates, especially at the moment of separation.

Here are a couple of crystal images from my latest chemical combination. B-Alanine and L-Glutamine were dissolved in Vodka and Water and allowed to dry without a coverslip on a slide. Image stacks using a 10X objective under polarized light, with a plastic wrapper used as a retarder. balg sep stack ...

This Frontonia was found in a freshwater sample from a stagnant pond at a winery in eastern Thailand.

Thank you all for the kind feedback! The stack was shot using a Sony A9 so the images were shot in a rapid series, but not video. The image is sort of hand stacked or stacked in batches. I decided what features I needed to include in the image (food, edge, surface texture, nuclei, food, pseudopods,...

Wow such an amazing image, thanks for sharing here. Did you stack this from video frames? I would assume so, since the Amoeba must have had some movement.

I use my DSLR a bit differently than you are imagining (I have a Nikon D750). I connect my DSLR to a computer monitor via the camera's HDMI out, and record directly on the camera's SD card. I share one monitor between my computer and camera, and while my monitor has multiple inputs you could achieve...

Useful for lower magnification ( up to 400X and maybe 600X) for thicker specimens. Pricey for advanced optical choices. Challenging for illumination techniques other than BF and potentially expensive. Unless you were heavily involved in thick aqueous samples a lot, probably not worth the outlay, un...

tlansing wrote: ↑

Tue Jan 09, 2024 3:41 pm

Hi macnmotion, nice video! I have seen the same thing and I didn't know what to make of it at first. It is the formation of a new mouth as part of cell division in Stentor.

Thanks. I love your video, now I wish I had continued observing the Stentor!

I found this Stentor in my pond water microorganism aquarium. The undulating rounded ridges along its side were something interesting to me. Is there any way to identify this? Also, at the beginning you'll see a piece of it separate as it gets wrapped around debris. Is this part of its reproduction ...

Francisco wrote: ↑

Sat Dec 30, 2023 6:57 pm

Outstanding work.

That's very nice detail! Well captured.

Very nice, you're off to a great start with stacking

Great captures

Very nice!

Topcode wrote: ↑

Sun Dec 24, 2023 4:29 am

I see, mine only makes about 1/8th a turn, so I guess thats probably broken... yay!
I'm also now noticing a small clicking noise when turning it, thats probably not a good sign either.

Mine only moves about 1/8 of a turn as well.

This is mine. Similar to yours I guess, but with a lever attachment that is used to turn it. Mine moves smoothly throughout its range.

My TMD has a lever, not a knob.

Wow. Very encouraging to see your tank set-up. I have been thinking about doing the same thing. So far I have not yet found a good book, or PDF, about creating a pond terrarium/vavarrium specifically for microscopy specimens. I am sure that info is out there, but not yet found it. I bought a book E...

I collected some water from a decorative pond feature at a hotel in Western Thailand (Kanchanaburi, along the River Kwai). Mostly the usual suspects, but here are a few of the organisms I've recorded this week. Watch them on YouTube for their best resolution: 1) Vorticella played back in slow motion...

I suggest, as does Phil/apo, learn to use your oil-100X objective..be selective..do what you enjoy with 20X, 40X, 60X dry...save 100X-oil for last excitement of your designated treat organisms on a wet mount slide. Much more use of 1.5% methylcellulose viscosity tool added 50/50 with pond water sam...