MicrobeHunter.com Microscopy Forum

Your iris has to be a condenser iris, so no field iris but I suspect that those rings are the field apertures for the two higher objectives, essentially preset field apertures, instead of an adjustable one. The only value of the field iris in establishing Köhler is that it gives you something to foc...

How far is the iris from the condenser front lens ( the one on top) and and are there any lenses between the iris and filament?

Let me guess. You're favourite band is Blur.

The little I think 3 watt bulb backlights it onto the field. You can remove it. There are caps to put over the hole. The halogen transformer has to be equipped with a Y on the output line with an extra socket. With the incandescent illuminator, there is another line and socket shunted off of the lin...

it's because there are tons of newbie microscopists that know nothing about microscopes. They just know brand names. There are the telescopists, people coming over from astronomy that think they understand optics but they only understand eyepieces, doing things like plunking a 2 1/4" Nagler eyepiece...

I would have to think on how to image mis-alignment within a condenser's optical path a little. That post was primarily to do with aligning the mechanical parts of a condenser to the lens axis, which would be a line drawn between the center of the front and back lenses of the condenser. I just assum...

I can think of numerous answers to that, the most obvious being that the design as an add on is quite expensive and that you would likely need a different one for each objective due to the correction tuning required.. Modern objective families are more harmonious but then they are the least to need ...

I go back to my comment that you cannot make a silk purse out of a sow's ear. Microscope imaging is based on resolution which can be theoretically ,mathematically determined based on the angle of incidence of the light. An N.A.is determined. Higher N.A.'s provide more information. More information i...

Those rounded cast horseshoes were common to many brands at certain times in certain periods. They seemed to be on Tiyoda most often but I suspect that there was a casting plant that churned out a series of horseshoe bases for just about everybody in Tokyo. The rounded stage too is a feature that sh...

You won't find a new Japanese made microscope within your budget. Kyowa and Meiji Techno would be the cheapest. Kyowa has a factory in the Philippines. They may have diversified significantly and the last new ones I saw for sale had either Chinese or Indian made objectives in it. Seemed like a good ...

A bigger picture would give some further clues about it's origins if you are interested, Clifford. . Japan's postwar optical industry had several big players and numerous smaller ones .The larger ones such as Nippon Kogaku, which adopted the Nikkor and Nikon brand as well as Nikko, Nikkorette and ot...

Also, look up Bestscope. They sell many of the currently made Chinese models branded under their own name or not. They also will source accessories. I bought a supply of of quartz slides and coverslips from them for instance, for peanuts compared to the standard industry suppliers. They seem to have...

It is compensating for tube length by focusing the image at a specific point in the tube. I don't use a 1/3X lens isn't in this system with an APS-C sensor. JW will obviously have to fiddle with the C-mount camera and I think get it closer. I don't know about direct projection with AO/ Reichert opti...

I looked for that link but cannot find it. At the time, about two weeks ago, I was searching for 15X eyepieces I think and that listing showed up way down towards the last page. The listing was something like Unknown trinocular head with eyepieces , so that explains sort of why it popped up under my...

keep us posted.

What happens if you put your Hayear camera very close to that exit lens? I have seen an old illustration or photo of a Polycon or Polylite with a C-mount vidro camera sitting right on top of the microscope. It probably had a dovetail to C-Mount adapter right there. I would think that exit lens has t...

See if you can adjust it for Köhler and see what happens. At least you know how to get a filament image so you can center it.
It's not possibly the partially closed condenser iris in focus, is it?

Congrats. Definitely an improvement. The Diagnostic Instruments photo tube alone is worth a bundle.

Is the vignetted field perfectly uniform ? does it have a fuzzy or sharply defined border? The condenser should be focusable for Köhler, which should defocus the filament image. The sliders are just to obtain phase, otherwise you should be able to to fo BF o.k. I came across a parts head and eyepiec...

You are maybe missing the point that any objective whether cheap or expensive should be symmetrical . The fact that each objective has a serious skew to it's presentation of ca wavelengths across the field to the point of flipping the polarity midsection or should I say maintaining the polarity rath...

I expanded the 10X 40X and 60X to the maximum on my screen and your chromatic aberrations are all over the place and as before completely unsymmetrical as well. . Firstly, the 10X shows an extremely yellow ca in the center but as you transit to the periphery the blue band is internal of the graduati...

There should be no aberrations at the longitudinal axis and any ca should be symmetrically radial with hemispheric inversion of the colour sequence. That is normal. Remove the condenser and post a cropped photo of your micrometer with the highest magnification objective that a condenserless illumina...

Then test the imaging with the abbe condenser, if it produces a reasonable Köhler adjustment and post some photos.

That .32X fractional lens is to compensate for the tube length and reduce unwanted alterations in W.D. It isn't needed if the tube length is kept to something close to the reference length. You have your SLR up pretty high in that picture. I would be inclined to get it down much lower. Your stated i...

In my experience, such unsymmetrical CA might originate from a misaligned condenser. This is true but he has an achromatic condenser and referenced köhler. Usually, coming from the condenser one would expect a subtle enhanced spread of ca unevenly into a region of the field, kind of like those clas...

So, " the lens that came with the Polylite SC " might be the telan lens, unless you can find it elsewhere back in the bowels. There will be an equivalent one 182mm back from the edge of the eyepiece tubes. It is just there to convert the infinity beam to a convergent beam. From that lens surface on ...

"mis-aligned or delaminated telan lens", I guess you mean the tube lens. But several other images/videos I saw from the same model show the same artifacts. I suspect those acro objectives were optimized for contrast, not for ca." No. Then those videos are showing images from a defective microscope u...

Then there is something optically wrong in your system. The objectives, which are planachromats will have some residual ca yes but it should be symmetrical. Achromats are naturally undercorrected, so should show slight yellow banding to the outside of objects and slight blue banding on the inside ed...

Since you are posting images from a camera mounted on a trinocular, is the same level and spread of ca, evident through the eyepieces?

Those micrometer images . Are they a center crop or if not can you provide a dead center micrometer image?