MicrobeHunter.com Microscopy Forum

23mm eyepieces.

I have a Canon 50D aps-c fitted to the trinocular port on a Labophot 2.
Using an NDPL2 type.
It's not costly or particularly challenging.
Modded the existing tube which is only a soft alloy.

Rorschach wrote: ↑

Mon Jul 17, 2023 4:24 pm

Phill Brown wrote: ↑

Mon Jul 17, 2023 3:06 pm

Inverted scope and sample cleaning and preparation?

Actually, the Olympus IMT-2 is an inverted scope. But sample cleaning and preparation is an important topic to cover.

Or did you specifically mean sample preparation for use on an inverted?

I would be specific for sample prep for inverted.

Inverted scope and sample cleaning and preparation?

It's the 23mm type as fitted to Labophot era CF. My best guess is some optimising was done. Light path alignment is dictated by the condenser to iris, field lens doesn't have much adjustment either. Filament is only adjustable for length but has a little wiggle room in the socket. Getting everything...

Is there any chance there is shutter shock or vibration? Looks like some motion blur rather than resolution. Hi Phill, these are heavily compressed due to the filesize limitations of the forum - please check out the full resolution image here for all your pixel-peeping needs: https://i.redd.it/0v21...

Don't put 220v 50hz into a 110v 60hz.
Sure you can sell the objectives.

Is there any chance there is shutter shock or vibration?
Looks like some motion blur rather than resolution.

Almost all nikon eyepieces are cross-compatible, even stereo to compound on the 30mm ones. You just have to avoid the really old ones or s series student eyepieces. Some Nikon eyepieces have 3 detents that fit into the tube in the head, some don't. The detents are to stop the 100% compatible eyepie...

The Nikon phase 2 condenser should have DF which is excellent for up to 40x .65. Nikon 20x 50 plan is good. Nikon 4x is easy to find. The weakest link for the Labophot 2 is the power supply. LED is not an upgrade. Focus is repairable if it fails, usually from being picked up by the stage or transpor...

All excellent advice. A pre owned as new microscope should be possible to find at around 25% of its new cost if you wait for the right one. Although phase isn't everything its phase condenser can be a great option. Don't put valuable slides under your microscope until you have discovered how to brea...

Having explained that "I just don't like him" everyone was encouraged to double down and deal with the underhanded sales tactics employed by Vickers.
The catalogue lists the optimal eyepiece for objectives.

Edited.
Internal surfaces should all be matt black.
No grease spots or joints that could be reflective or allowing light in.
Lenses need to be clean around the edges, residue at the edge can make a bright spot.
Image capture can be a lot more sensitive to subject illumination also.

.

24 v,. or something not right if you input 220! Why 24v? The lamp is 12v. Putting 240v AC in the 110 setting has resulted in 0v output at the socket? Sadly what I would expect. Is there a fuse on the PCB? A good old school electrical engineer might be able to fault find what gave up first but it co...

It's doable.
There are a few roll pins that have to be relocated if I remember correctly.
The holes are already there.

Step -1, 6mm double sided tape and Bernina 930 with hands free.
For many layers of testy materials the basting stitch is good.
It skips a stitch but walks the material so the flywheel keeps good momentum.
Step -2, Check for ASD,got to love those special interests.

I'll leave oil immersion on an inverted. Doesn't that mean the condenser also has to be oiled? The inverteds I was cleaning are optimized for epifluorescence and confocal laser scanning of cell culture dishes, so the objective is the condenser. There is a diascopic condenser but it has very low NA....

I'll leave oil immersion on an inverted.
Doesn't that mean the condenser also has to be oiled?

Antartica wrote: ↑

Tue Jun 06, 2023 8:16 pm

DonSchaeffer wrote: ↑

Mon Jun 05, 2023 6:50 pm

What kind of lighting is this?

Just some simple post processing

I'm sticking with a laser pointer.
Cat toy pen make happy from china.

The Watson DG zonal oil condenser is at na 0.9. Either with the 50x .9 oil objective or with a funnel stop for 100x oil that is listed as a part. Provided the subject is illuminated and the na of the objective is below the na of the dark field it should work. If the limit is .9 anything over 90x is ...

I'm going to kick off with the suggestion it's a low power laser, interesting result.

Thanks for sharing.
Amazing as always.

Top work.
Great images.
Would be interested in a head on of the jumping spider also if it's possible.

Good luck. Sure you will see anything well enough with that. I wouldn't be without DF, should be possible to do with DIY but dry DF condenser for Motic is available and likely very good. You will need to enjoy subject preparation, pollen is good to practice with. Maybe get some diatom slides from eB...

Thanks for sharing.
I'm always impressed by the body of work you have produced and continued contributions.

Lovely clear images with no mention of the hours of cleaning glass.

It's possible a polariser could help the process. Would also be very interested to see some of the original images before processing, Some remaining D.O.F can give a sense of form. Still hoping 3D makes a comeback sometime, as the original system using LCD glasses was created with a single lens endo...

I'm seeing the stacking not coping. Great subjects. the last photo is in 3D for the glasses red cyan In the first picture the centre subject has some details that look like the software struggles to keep the form. It's possible that reducing the intensity of the lighting as with deep space objects ...

With a 6000k LED the difference may be preferable.
With incandescent it's usually the other way with a blue filter or ND.