MicrobeHunter.com Microscopy Forum

The fact that DIC is a polarized light technique, it would stand to reason that POL and DIC would be used simultaneously. Combining RL and TL contrast methods are certainly possible. RL Fluorescence with TL DIC or Phase or even simple POL is often done and certainly was one of the advantages of the ...

Keep in mind that the back focal plane was not a big issue because even in the era of finite optics, Reichert upper prisms for DIC had the ability to move the prism vertically relative to the back aperture. Meaning only one prism was used for all magnifications

All Reichert objective were built to a strain free standard so they were conditionally strain free. Those marked suitable for IK were selected as the most strain free and optimized for work in POL applications. . Keep in mind, that strain can be introduced simply be overtightening an objective in th...

The Series 100 was marketed as the parsimonious alternative to the Series 110. As pointed out, it had a different finish, the objective printing was different and if I remember correctly came with 18mm FOV eyepieces rather than 20mm. All in all, back in the day, AO was the market leader in hospital ...

Thanks for jarring my memory, On the Bio platform the 4th mag changer position included the bertrand lens, The 2x and or 2.5x was used on the Met and SC versions. The primary function of the bertrand lens was to view the back aperture of the objective for phase ring alighment.

As I pointed out earlier, the Leica Delta optics are a reference 200mm focal length w/ M25 objective threads. vs 180mm RMS for the Vienna based objectives.(Bio) As Apochronaut pointed out the US based objectives with the US eyepieces for the 45mm Reichert objectives had a different ca from the Vienn...

Lots going on since my last post, I agree scanning the lit will be helpful. Regarding Delta optics. Objective thread size is different from the DMR and the Polyvar and the reference focal length is different as well as lateral color correction in the tube lens. An image could be formed, but would no...

From the 80's onward, the Polyvar that were in the field morphed into the into the Polyvar SC and further iterations used in the Semiconductor business. Leica spun off a separate company that focused solely on the Semi Business including Lithography systems and other tools for fabs. . The Bio and st...

I finally dug into my Reichert catalog to try and find this objective. I recall it in the program for the Polyvar, but can't realize the details. I have reference materials for the Life Science Polyvar, and Polyvar 2 as well as the Polyvar Met, Polylites up to the 88 with literature from 1983 to 199...

Centering keys are used for the annular diaphragm adjustment in phase contrast. Fine tuning of the DF stop could also be used. Otherwise, Fluoro, POL, BF will also function with the open aperture of the turret. Centering the condenser to the Field Diaphragm should be independent of the annular stops...

Any reflected objective of magnification less that 2x using coaxial illumination would need a contrast cap. Essentially a 1/4 lambda plate that is rotated to achieve the best contrast. This is also requires crossed polars in the illumination axis. With out these devices, contrast is seriously compro...

I took a closer look at the DIC slider and I am fairly certain it is for an MeF3/4.

Sorry I haven't been on the forum in a while. Here are a couple of update points relative to the Univar/Polyvar. If i recall correctly, the Univar was introduced about 1973 and the Polyvar approximately 1975. The major difference is the Polyvar required phase specific objectives with annular rings o...

I do have a DIC slider, but I am not sure if it is for the Polyvar or the MeF4

I wonder if you could modify the Aux Lens from the AO Seriese 10/110 abbe condenser to work. There should be bazillion of them out there cheap and would require mostly modification to the mounting point.

Quite the impressive collection of Zetopans spanning a number of iterations. the silver is the latest vintage while the light cream color was done for the exclusive distribution by Hacker prior to AO purchasing Reichert in the 60's. Black is the oldest. I see the silver one has a Hacker label on it,...

Impressive set up. And Clearly the price was/is right. There are two contrast condenser offered in my reference material. 9 99 00 05 Contrast condenser, N.A. =0.98 turret with 6 annular aperture diaphragms (1-6) 3 empty apertures, centering deice, aperture iris diaphragm, with wide field optics, on ...

I have the 1978 version of the Zetopan order guide. This is from the last year of production and availability, as the as the Univar (1974-5ish) and subsequently the Polyvar (1976ish)were being brought The only reference to the UV condenser is for use with the Binolux illuminator for fluorescence and...

The short answer is yes, DIC is possible with the Polyvar. I have two universal condensers with prism and phase rings for the Polyvar. I also have a couple of objective for this microscope. I may also have a Polyvar biological nosepiece. Yes, it only used one objective prism for all magnifications. ...

Is this Polyvar a Bio version or the Met version? I know I have the universal turret condenser for the Bio version with the Phase and DIC prism. I thought I had a stage, but have not located in my storage yet. Are you located in the US?

You really don't want to remove the excitation filter. Although about inch of glass will prevent the transmission of harmful UV rays, the intensity would overwhelm the retina. In addition, the emission from autofluorence is still wavelength dependent. In otherwords it needs the excitation to generat...

Thanks for the comment and good luck with your investigations.

The Biostar was never offered with Leica branded objectives, although it was infinity corrected so someone may have built a system with objective from an DM IRB. On this point I am positive you are wrong, to the extent that it matters. I never bought an 1820 from the manufacturer, but you frequentl...

Phil, I agree completely.

The Biostar was never offered with Leica branded objectives, although it was infinity corrected so someone may have built a system with objective from an DM IRB. However, it would require RMS/M25 adapter rings to fit the objective into the nosepiece. Of course, it would have been possible to mount L...

The article referenced in association with this objective specifically mentioned multi - photon applications. Terminology associated with Confocal Microscopy. I suspect the low mag is desired because of thick tissue samples and the high NA is beneficial because it will aid in presenting an intensity...

I had some old literature that provided some detailed information. After the specs is my commentary of the applications and image information. 1711 was used with Auramine stain. (436/450/GG475) This was common for TB bacillus that had excitation in the 436nm range and emissions in the in the yellow ...

It seems this objective would lend itself to applications with confocal microscopy. High NA provides better light amplitude combined with higher DOF associated with the lower magnification. Thicker samples could be viewed, while eliminating the noise in the interstial tissue areas.

This is a great discussion when it comes to comparing theoretical vs. practical real world application of the condenser diaphragm adjustment. Most of the major manufacturers have used some form of reference to the objective, in use, on the condenser, giving the user a reference point. It doesn’t mat...

Pete,
Sorry I don’t know the specifics on those objectives. It might be possible to search for the spectral curve on each objective, ideally using the cat number if you happen to have it