MicrobeHunter.com Microscopy Forum

Hi Sporacle, welcome.
Agree with gekko, your girlfriend has opened a door into a very interesting world for you.

Was a little worried by the low end of the graph but after your comment I looked at it again and realized that no part of it was below 400nm, and at 20% Relative Radiant Power it was up to 430nm. So I feel a little better. In the same dimmed room, I aimed my microscope LED at the same shirt, no reac...

Most camera UV filters apparently do not filter out most of the UV radiaion (one should check the transmission characteristics). Your mention of UV camera filters got me looking. Turned one up among my old camera stuff. This is what it reads: U.N UV 49mm JAPAN No other markings so unknown brand and...

. However, I worry a little about ultraviolet leakage from the LED, so will probably end up using some UV filtering which will ultimately tint the light somewhat. CE Have you found a UV filter to use with the LED lighting? Would like to know how you chose one. Can one measure the amount of UV the L...

Interesting images. The 40x seems to work well for this.
Some of the RBCs seem to be joined (in the second image) by 1, 2 or 3 lines.
Is that an artifact or are they effectively joined?

Yes and (possibly) no: Would I be wrong is I said that your (15x X 1.25) = 19 allows you to see the magnification that your objectives are providing rather than anything extra? I mention this because I have the impression that Dale seems to think that going from 18x eyepieces to 20x eyepieces will ...

I should have mentioned my scope will also have a set of 18X eyepieces, so with a 20X I should have a lot of quality resolution flexibility. I'll go check Ebay for a 20X. Think you will find 18x does not increase resolution, 10x eyepieces are what you want. Any increase in meaningful magnification ...

This old pickle jar was the perfect solution...

Hope this does not mean you are giving up wine!

I very much agree with what I think is implied by 75RR's statement:

I think gekko and I are on the same page; the Cree XML2 is closer to the original tungsten filament size than the XTE.

Just ordered the very same controller from China. Thought that if I needed advice it would help if I had the exact same one as you. Will take a little longer to get here than it did for you, probably about 4 weeks. The 2 Cree XLM2 I ordered cost me $11.80 including an additional $2 dollars for a tra...

I remember Liebfraumilch as a rather inoffensive white wine.
You seem to have found a stronger vintage. Hope you managed to rescue your steam iron.
Agree with gekko, they are sharper. There are a lot of interesting alga out there waiting for you to take their picture.

That is great resolution and images.
If I remember my Frankenstein correctly you should be ok unless you build a female Frankenscope for your male Frankenscope and then try to steal her from him, or was that a film?

Many thanks for moving the video (saw it on youtube) still do not know why photobucket did not work for me, I use it to link photos to microbehunter, works fine for that. Very clear nice and video, you caught the brownian movement within the alga very clearly. Very smooth light intensity movement. Q...

Many thanks for the photos CE, and the amazon link. Did not realize it was that small (hand photo)! Do you have the transformer set at 9v? I suppose any where between 5v and 35 v will do? The controller (amazon link) states: Rated Output Current: 3A Does that mean that it would reduce the current fr...

Thanks CE, that would be useful or at least calming! The pdf from the Cree website is 12 pages long and does not mention ultraviolet light at all. Worries me a bit too. Using a common or garden led flashlight at the moment with my Zeiss Junior. Had hoped the Cree would be safer. Do you thing it migh...

Thanks for the update. Do you have any photos of your "contraption"? What transformer do you have hooked up to it? Volts/amps? Just picked up a couple of Cree XML2 which I ordered from China. Pdf from Cree website says max drive current 3000mA, Reverse voltage -5 v Not sure what the negative voltage...

Like the images.
How thin do you manage to cut with your microtome (they look pretty thin)?
Would second microgreen, photos would be nice. Was/is it difficult to make?

Given how small Scenedesmus are, your second photograph is very sharp. Well done.

Does anyone clean their slips, or just toss them?

I do. I use my thumb and forefinger, skin does not scratch.
I use washing up liquid to clean them, rinse the slip well and then I wash my hands with an anticeptic.
Use creolin at the moment.

... hope you will keep us posted as your system is finalized. Not quite finalized, but closer. Enough for an update anyway. Picked up a transformer today, should allow me to use the incandescent bulbs and with a little modification a Led. 2v – 12v AC and DC 5A in 2v steps. http://i1368.photobucket....

Interesting image. What the limpet like objects?

Well done. If I may make a suggestion - a photo taken with this setup of a biological specimen would round off the article nicely.

Can't be much help I am afraid, other than to mention that you budget is a little low. If you bring anything in from abroad, shipping costs and import duties will make a big hole in your available funds. That leaves a local buy. As to viewing on a PC, there are USB cameras that drop into the eyepiec...

I also tried glycerol (not as a mounting medium as seb uses it, but to increase viscosity), also with variable results. Picked up some yesterday for that very purpose! Will let everyone know how it goes. It is hydroscopic, so I imagine it is not suitable for use on certain subjects unless well dilu...

Playing back the video, I did not observe any interference patterns at all in the image.

Very promising. Looking forward to further reports. :)

Hi Verona,

As for microscope - I even dont know what microscope I use:) as it was a gift

All the more reason to post some images of it in the "My microscope" section. Welcome

Really looking forward to this.

Thanks. It remains one of my favorites. :)

Very nice. Would also like to add a special thanks for including shutter speed!

Also looking forward to seeing some more. Given the size of the bottle I imagine you will be able to photograph them until the cows come home, if you wish. Great opportunity to perfect your Haematococcus technique. Have you looked in the other bottles yet, or are you waiting for them to thicken a bi...