MicrobeHunter.com Microscopy Forum

Thank you all for your inputs! I think it is probably an interferometer objective as Scarodactyl suggested. It looks like a Mirau-type objective as shown in Figure 5 at https://www.microscopyu.com/microscopy- ... rferometry

I recently came across this objective while looking at a disused GE DeltaVision OMX TIRF-SIM super-resolution microscope. Screenshot 2022-05-14 at 18.47.33.png Screenshot 2022-05-14 at 18.47.13.png Screenshot 2022-05-14 at 18.46.38.png Screenshot 2022-05-14 at 18.45.47.png Has anyone seen this befor...

I just read an interesting paragraph on Nikon MicroscopyU about an unexpected positive effect of not thoroughly cleaning immersion oil when using heated objectives for live-cell imaging, especially on inverted microscopes where the oil can flow downwards onto the objective barrel: "The only negative...

Here's a collection of Frank & Ernest microscopy cartoons:

https://frankandernest.com/search/index ... microscope

There is a good chance the carpet was cleaned at some point in the past. Much of the history could already have been lost or hard to trace it's current whereabouts, also it could have been cross contaminated with landfill waste. That would certainly make the analysis even more problematic and inter...

My strategy was to add a vertical illuminator to an existing transmitted light microscope so it can do both metallurgy and biology (and others). This might not be cheaper than getting a purely metallurgical microscope, but it could be "economical" in the sense that you have an instrument that is muc...

I think this could be really interesting as it appears to give a lot of potential scope for identifying different types of fibres (synthetic vs natural, what synthetic materials they are made of or which plant species they came from) using the crossed polarizers and retardation plate (or variable co...

Hi Milou and Idflan, No problem, I am glad the polyurethane was of some interest. Answering Idflan's query, it has been three and a half years since I made those slides and they still seem fine and unchanged (I examined one of them under the microscope recently). I keep the slides vertically in a sl...

https://photos.smugmug.com/photos/i-fhfNxk3/0/75c85ef2/L/i-fhfNxk3-L.jpg I have used ebony-coloured polyurethane wood varnish with some success on slides of paper fibres mounted in 99% glycerol. Glycerol is not really a permanent mountant so I needed something to seal it. I didn't want to use nail ...

It's been about three weeks since I put the slide out near the front door with a drop of olive oil, and I put it under the microscope yesterday. There were particles and fibres although not very many, as Singapore is a city with relatively good pollution control of particles of optical microscopy si...

Welcome! I read with interest Dubious' comments about PWM - so that's how the bicycle light adjusts intensity! Perhaps one could use a couple of neutral density filters in this case to adjust brightness, while keeping the LED permanently at full power? I do this for my tungsten halogen lamp, as it h...

-- I tried measuring the depth of a slide, using top and bottom markings, without a coverglass. The slide was 1,130 μm. I had to rotate the FF knob so many times that it was hard to count, so I abandoned that and went back to the coverslip marked on top and bottom with a marker. There are tiny spec...

Please forgive me if I have misinterpreted your description, but : It appears that you are trying to measure through glass. … if so, your results will inevitably be distorted by the Refractive Index of that medium. Thanks for mentioning this. It prompted me to take another look at the reference I w...

On my Radical RPL-3T, the coarse and fine focus knobs are not only separate but also turn in opposite directions (for the same direction of stage movement) for unknown reasons. This makes it very confusing and annoying when you switch between the two and also increases the risk of accidentally smash...

You may also be able to find the information in the microscope’s user manual. If you need to be sure of accuracy, I recommend calibrating it using a broken coverslip to see how many graduations of the fine focus knob it takes to get from the lower surface to the upper surface of the coverslip using ...

One last personal thing on design. I loved the large, square, oversized designs of the the late 70's and 80's. Check out images of the following... Reichert Polyvar Carl Zeiss Jena Jenaval Contrast Carl Zeiss Jena Jenaluminar Carl Zeiss Jena Interphako Where are the original castings? I want them!!...

Thanks for a very informative and interesting topic. I have little knowledge to contribute but some of my personal experience. A few months ago when I was shopping for a polarizing microscope, the Nikon Labophot/Optiphot and the Olympus BH2 series were the two contenders for me. Two things won me ov...

I use DaVinci Resolve. The selling points for me are: - completely free version available - very powerful (they use it to make Hollywood films) with all the capabilities built in. It doesn't lack some critical function you suddenly need for a particular situation, unlike certain open-source applica...

I use DaVinci Resolve. The selling points for me are: - completely free version available - very powerful (they use it to make Hollywood films) with all the capabilities built in. It doesn't lack some critical function you suddenly need for a particular situation, unlike certain open-source applicat...

Enjoy your next great adventure. Before you go, let us know what you find with the parallel polarizers (in the other thread) if you can! Come "visit" us often!

I couldn't find the supplementary information linked to in the paper so I wrote to the author. He explained that the journal hadn't uploaded it yet and kindly gave me the following links in case anyone is looking for it: GitHub page with complete instructions for building the Lego microscope Supplem...

Now if I can only find a way to adapt the vertical eliminator Love the frankenterminology :lol: I've been thinking about maybe 3D printing a adapter, anyone with knowledge on what this would require? Here are some considerations: - how precise the dimensions need to be and does the manufacturing me...

For my latest mini-project I decided to explore feather microstructures, which are sometimes used by forensic investigators to identify bird species from the feathers left behind. Although there are lots of micrographs of feathers in general, very few of them actually show clearly the nodes and pigm...

Thanks. I think it's quite relevant to the topic, going by the title of it. Apologies if not - I started off this digression!

Thanks for the idea, I’ll try it! Do you know if there’s any kind of strain-free adhesive to make particles stick better and not get blown off the slide by wind?

Well I just found a collection of paint micrographs: http://www.microlabgallery.com/results.htm?cx=001258474697822866463%3Awhoad_i24ri&cof=FORID%3A9&q=paint&sa=Search There's a reference in one of the links - Crown, David A., THE FORENSIC EXAMINATION OF PAINTS AND PIGMENTS, Charles C. Thomas Publish...

ACRYLIC PAINT IS PLASTIC BASICALLY...IF THE DRIED IN THE AIR, AND MOST DO YES THEY ARE ROUND... TRY SQUIRTING SOME SPRAY PAINT 6 FY OVER A SLIDE AND SEE As you already have lots of spray dust at your place, it would be great if you could post some micrographs when you have time - if they're acrylic...

I thought it might be worth mentioning this home-made Berek compensator made from a mica plate, which rotates to give variable retardation.

Patta - thanks for the story and images - I enjoyed them!

I'm wondering if it's possible to give more space to those who need it (either automatically or on request) while reducing the quota for those who don't. My PM inbox is near-empty and I believe it will remain so, and I'm happy for my unused space to be taken by someone else.

I think that, if indeed condensation is likely (for any climatic/environmental reason), keeping the microscopes warm is the most practical and economical way. Less nuisance than dehumidifiers of any sort. HEPA filters are an overkill, IMHO. I can't prove it definitively but my impression is that in...