MicrobeHunter.com Microscopy Forum

Does the small gear separate from the larger one? What is the original made from? It's brass right?

Hi Saul, What workflow are you using with such a set up? As in, do you zero at the lowest level and then motor away while video-ing, then use toupview to z-focus the resulting movie file? I'm curious what people out there use for software/controllers and how they prevent crashing the objective. Than...

Speaking of the Nikon Plan APO 20x, this guy used the VC version for UV spectrum to zap images into glass:

https://youtu.be/_w0Z2Y5vaAQ?t=315

Notice that the filter cube has an angle to the top filter the same way the AX polarizing slides do:

https://www.researchgate.net/figure/Cut ... g2_5250836

JWW wrote: ↑

Sun Feb 02, 2020 6:47 pm

Polyvar SD (semiconductor)

It's a Polyvar SC not SD.

Bubbles will arise from shaking the Norland bottle before using, or from applying in a manner that adds bubbles as you apply a drop to one surface; both these errors are easy to avoid, especially if you practice applying a drop without having the top of the drop exhibiting tiny bubbles. But if you ...

Do you have to put the resin in a vacuum to remove possible bubbles?

So the DIN 6x20 is a standard for compensator optics. Mentioned a was down on this web page (https://www.mccrone.com/mm/senarmont-compensation-how-to-accurately-measuresmall-relative-retardations-0-1/). I'm curious about more details though if anyone has any. Thanks. I'll have tested a lapping polis...

Does seem like it is just for the frame being deeper. Didn't know about the 88 meaning 8". I'm curious if the Leica DMR's and the Reichert objectives (late 1990's to early 2000's) need lateral correction lenses/relay-lenses for the eye-pieces and camera trinoculars. There are no drop in relay-lens f...

My question is more along the lines of why the condenser gets different prisms for different magnifications but not the prism between the objective and camera.

Hi, Regarding transmission DIC, why/how does the Olympus BX level of microscopes have different magnification DIC prisms for the condenser (U-UCDB-2), but one DIC prism to that goes into the objective turret (U-DICT) used for any objective. Does the U-DICT slide allow it to move the interference pla...

Basically it's a motorized microscope, motorized polarizer and a laser to zap data into a large petrographic blank slide.

https://www.pcmag.com/news/thanks-to-mi ... s-of-years

Thanks. I will try it out. Which direction do you think this one would have given the text you are referring?

I'll check this weekend.

Thanks,
Ted

Thanks. I ordered a copy of the book. But if you dig up your copy anytime soon, I'd be curious what the scale of some of the numbers were just to get an idea. The distance to the interference plane is supposed to be shorter for prisms aimed into a condenser versus objective.

Given a block of calcite, what is the best way to accurately find its optical axis? Would it need to be polished to find that? Ideally it would be nice to shoot polarized colminated light through, motor an objective around to find the maximum output plane. I'm still vague on the optical axis aspect,...

At first it seems that the angle cutting the calcite and the thickness are not the main factors, as much as the angle of the optical axis one of the two attached halves. So unlike Wollaston prisms, the distance to the interference plane seems to be the main issue. So measuring the distance from that...

If anyone finds pointers to articles to calculate the theta angle based on something like objective magnification or something else, please send me pointers. Info appreciated.

Here is a link to patents citing Nomarski's 1960 patent:
https://patents.google.com/patent/US292 ... 2+#citedBy

Hi, I have some CNC setups built, and there are some people I work with here with their own setups to consult. As a goal for 2020 I’d like to expand that to grinding and polishing. So I’d like to polish calcite flat and parallel, and then at opposing angles to make DIC prisms cheaper than used ones ...

If you are looking at Radical, I'd avoid them unless you know someone that bought a particular model and was somehow ok with it. I bought a "Radical 1500x Trinocular Steel Aluminium Alloy Metal Testing Lab Metallurgical Reflected Light Microscope with Polarizing and XY stage" off of Amazon in 2018, ...

This is probably one of the more complicated contouring curves I’ve done so far in Fussion360.

This is the lower half of a Zeiss AxioPlan 2 microscope filter cube.

This is the AX-PO with an angled polarizer. Note the arrows in the pictures show alignment going the length of the slide, and that is the angle of rotation.

So I'm still trying to get the hang of Fusion360's drawing option. But I got the AX-AN slide I think reasonably accurate.

Maybe it is for the same reason ZYGO tilted these parallel glass plates as shown here in figure 3 where a "small tilt angle for both plates directs unwanted reflections off axis, where they are blocked by internal apertures,..."

The BX slides do not have angles on them.

Finally assembled a complete collection of AX slides.

Note how the polar filters are mounted at an angle.

I hadn't realized it, but eh condenser lens has S55 written on it. There are annuli with S55 written on them. So hopefully this is a match.

So this is what it looks like so far. You can see the crack. An almost closed holding C metal ring snaps next to it from the short side. So a ~3mm gap is milled into the black holder for the glass and ring. The glass is glued somehow. So why a ring? Also the surface seems pitted. It feels smooth, bu...

I got the optics window way cleaner. It still has smudges that are not coming off without a lot of q-tip scrubbing. The glass seems smooth, but has a crack. Maybe the crack was from a high temperature change. Don't know. Since it's crack, I'd like to replace it. But it seems glued in there even thou...

I think they all have screws holding them in. No dovetails.

If anyone finds meaning in the R, X, E, and B; I'd be curious to know.

The objective turret is removable and has the turret built in if there. Is the DMRB you mention with its original objective turret? You can buy parts and put them in these unit to add features to a reflective axis.