MicrobeHunter.com Microscopy Forum

Well. I have the shakes, Pete. In my case it seems like it has been coming on as part of the Borreliosis circus. That tiny needle in a pair of small vicegrips ended up being the primary tool for moving the lens here and there until I was ready to install it. I used the needle with a tiny hardened ep...

You are correct, I got the number wrong. Coax illum. Anyway, what about those windows behind the access plate? How critical are they? How to adjust? As it is, I just set it up at the small machine bench with the lamp bracket hung underneath the body bracket on the boom. Should work fine. But if any...

I didn't check it with a bertrand lens but I will once I put it back together again. The last picture shows the lens stack focused but as I backed off the focus, the de-lamination wasn't apparent, which surprised me. It seems, that at the point the cone of light is passing through that smear of wonk...

A few more pictures. Picture 6 is the inside of the front lens cell with the lens heavily cemented to secure it and keep it aligned. The initial analysis of the objective indicated that it's sole problem was the detached front element, however upon separating the other 4 lens cells , it was immediat...

One of the forum members, Jim Wheeler, known here as apatientspider asked me if I could attempt a repair of a fairly old and unfortunately non-functional Spencer 90X 1.4 N.A. apochromat. The front lens, otherwise undamaged was floating free, having departed it's housing. I had done this a few times ...

I think it is safe to say that almost anything is possible when it comes to bacteria. Communal organization, bioslime chemistry, aggregation , agglutination, shapechanging. Some of the most startling facts about them has only been known for 10-20 years or so. Anyone studying them could be viewing ne...

Those are pretty small diameter lenses. I would lean in the direction of them being 16mm movie camera lenses.

I might have one but I can't relate it to the model 1380 or ES. If you post a picture of yours, I could tell.

If I was you I would give that 22X water dipping objective a spin. With a plezy adapter, it might be very useful for viewing water saturated samples; raw plant tissue and the like. It looks to be a dipping objective, not a water immersion objective. Is the 10X a water immersion objective, Pete? Leit...

An additional problem with living organisms is the sample thickness many of them provide. An individual organism or matrix of organisms, either way , ends up requiring an objective with an unusual depth of field in order to visualize it accurately. Deep samples allow an undesirable amount of travel ...

There is also this one .
http://www.science-info.net/docs/reiche ... nglish.pdf

I have used an Axiomat. They are quite impressive and truly modular. The f.o.v. with 10X oculars was 25mm, I recall and it could take 4 sets of oculars, one on each side of a more or less cube shaped frame. There was facility for all film formats, 16mm film and video. The mag changer was a little ex...

Very illustrative tutorial, Bill. I might just treat my phone with more respect, now. I probably won't leave it on the van roof anymore, at the very least but that might be determined by factors outside the realm of logic anyway.

The Axiomat does have unusually large threads for all objectives and as a result a stunning f.o.v. The program was discontinued because the instruments were just too expensive. Everything on them is huge. I know a guy in Toronto who specializes in them. Has parts. If you want me to connect you two, ...

The second ingredient on the list is starch, which in addition to being a binder, absorbs water and fat, keeping it in suspension. After a period of storage the fat and water become available to yeasts which begin the process of consuming the sausage for you, by initially feeding on the sugars, alth...

It also depends on what kind of sausage they are. I am assuming that you are talking about some form of cured sausage. There are two ways that cured sausage is judged to be "cured". For some, those that have been given a limited fermentation, it is based on water activity. The lower the water activi...

The B & L U.W.F 15X are not really rare but they are uncommon. They are very large with a 25mm eyelens and perform much better than the # 147. There is nothing really wrong with the 147's, supplied as original equipment but everything has a cost. There is only so much they could engineer into them a...

The 3 and 4.2X AO zoom scopes are a little optically simpler than the model 580 6X , which in some AO literature is described as " high resolution". I would have to say, I agree with that but the resolution difference is not huge. The Cycloptic has yet an edge in resolution over the 580, largely due...

Those are more likely a yeast growth. Yarrowia Lipolytica is a common one which feeds on the fat. There are many others.

Glad you have ruled out your orbs. AO got into some cement problems with the Seidentopf heads for the 100 series. The right hand, or straight through beam should have more colouration from cement failure in the beamsplitting prism , than the left or reflected beam. The failures I have seen do seem a...

Tip the microscope down on it's side and have a look with the ocular tubes/eyes reversed . That will tell you if the colour difference is physiological.

How about plywood and a scroll saw. Even cheaper. You can probably find the plywood in a dumpster and borrow the tools from a tool library.

How would you determine if it was strain free? If it was empirically, and you found your repair not to be , then you might be happy to have chosen to use a cement that is relatively easy to dissolve and clean. Balsam would be a good choice for that, since ethanol works well and is fairly easy to com...

It the calibration look more related to transmitted D.I.C. or reflected DIC, rather than transmitted, D.I.C. or reflected. Possibly to get the correct distance to the rear focal plane of the objectives, a parameter upon which D.I.C. is based.

Following on with the previous test, since everything was set up, decided to check the magnification and performance of two other antique objectives, the Spencer 95X achromat from the mid 1920s, and the Spencer 95X fluorite, also from the mid 1920s. First up was the 95X achromat. Image quality was ...

Fast work. thanks. If the Leitz has a particularly good image, then it might have been made with some fluorite lenses in it and that might account for it's higher than normal magnification for a 2mm focal length. Mine have images that in addition to being under magnified seem to be in need of furthe...

I have an old AO stage micrometer slide, and a pair of Cat 146 eyepieces, one of which has a reticle. These came on a Cycloptic. In the next few days, I will test my old Leitz objective marked 1/12 and 100X on my 4 Series stand. The tube length error from the Leitz specification will only be about ...

Hi Phil, I don't have an explanation for the unexpected behaviour of the Leitz objective. The only thing I can imagine is that the front element might have been exchanged for an other one. The front lens of a fixed oil immersion objective is easily damaged and someone might have screwed something e...

Departing somewhat from this thread http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=5&t=7096 and moving to a new one , where I have actually tested the magnification of a number of older oil immersion objectives and as well a few new ones. The claim of the pre-war Leitz objectives to m...

There are 60x /0.85s, but I think they'd be better off going to 100/ 1.25 oil. Hi, perhaps the following recent experience with my old, unknown brand (to me) 60X0.85 dry objective (marked only "60X 0.85") will contribute. It is of 37mm parfocality. I attached it to the nosepiece by means of an RMS-...