MicrobeHunter.com Microscopy Forum

The Axioplan 2 is the more current one.

Eye-pieces and nose-pieces from an Ortholux do not fit an Orthoplan.

Your pricing for the other items is at least ambiguous to put it mildly.

Right. No Darkfield with the Fluotar 40/1.30.

Don't compare apples with pears. The PL Apo 40/0.75 is a dry objective while the PL Fluotar 40/1.3 is an Oil one. Resolution is a function of NA. The 40/1.3 provides significant higher resolution than the 40/0.75. A PL Apo is perfectly corrected against chromatic aberrations while the Fluotar is cor...

By far to large for that.

This is hardened grease for sure. There is only one way to fix it: disassemble, remove the old grease, re-grease, assemble. The Orthoplan does NOT have the feature to vary the tension by turning the knobs in opposite directions. Both knobs should turn very easily if in proper condition. This jobs is...

The Leitz PL Apo 40/1.00 Oil, 170/0.1 Phaco 3 is an objective as good as an objective could be. One of my very favorites.

Adapting a mechanical stage depends very much on your needs.

What do you use with your Wilovert?

standard slides?
petri dishes?
well plates?
culture flasks?
other?

For standard slides it is easy. Just adapt some stage found on the market. For the other stuff it is a bit more complicated.

Good fitting heads are those from white Leitz Dialux or Laborlux microscopes. Their dovetail is 42 mm as well. The oldest Leitz microscope with a 42 mm dovetail was the Dialux 20 from 1978. Everything from a grey (43 mm) or black (41 mm) Leitz will not fit. Same with heads for high-end Leitz stands ...

The two pictures show two different dark field condensers. The first is a dry one suitable for objectives up to NA of about 0.7. The second one is an oil immersion condenser suitable for objectives with NA up to about 1.0. The handling of this condenser is somewhat tricky as well. Even with the latt...

I suspect that Wild and Hund objectives share a common heritage. I wonder how Wetzlar fits in this sequence..the names Wetzlar and Leitz are often seen together. Wild was a Swiss microscope make who merged with Leitz. The company acquired other makers like Reichert, American Optical and Bausch & Lo...

I had personal contact to Micheal Wagner at Hund. m.wagner@hund.de . There was no relationship between Hund and Leitz beyond the fact that both are based in Wetzlar. Hund acquired the microscope maker Will in 1988. This is how they entered the microscope market. You may use other non-PH objectives w...

Nice instrument! Phase contrast objectives are not interchangeable between manufacturers. You need Hund objectives. Web: www.hund.de Phone: +49 6441 2004-0 They are quite helpful for amateur users, not as arrogant as the big players. Hund parts are frequently found on Ebay, often on the German site ...

This ploemopak works fine with infinity objectives? Because they seems to be way cheaper and abundant. The Ploemopak is designed to work with standard transient light objectives made for finite tube length 170 or 160 mm. Infinity objectives will not work. To make it worse: In reflected light fluore...

EF and NPL objectives work on the Orthoplan. They are just not perfectly plan. If you can live with it, you are fine. About the Ploemopak: You need this for reflected light fluorescence. Your Orthoplan is already configured for transient light fluorescence. Start with that and look for a properly pr...

EF and NPL objectives are not well suited for the Orthoplan's wide field of view. It needs PL or Plan ones to feature a plan field.

Congratulations on your new microscope. The difference in size compared to the Amscope is just the difference between a microscope and a toy. :lol: The 402aa condenser is a very special animal designed for transmitted light fluorescence work. It contains 5 annuli. #1 is standard phaco 1 #2 ist UV ph...

Leitz made at least one phase contrast condenser with immersion front element, the 403c. But it appears to be a pretty shy animal. I do have a number of oil immersion objectives with phase rings in use. 25/0.75 Oel Phaco 2 Fluoreszenz PL Apo 40/1.0 Oel Phaco 3 63/1.30 Oel Phaco 3 NPL 100/1.30 Oel Ph...

Since nobody here knows your requirements ....

Tom Jones wrote: ↑

Wed Jan 11, 2023 4:43 am

It's easy.

Very much so!

To make blood smears permanent I use the following process. Make your smear the usual way. Let it dry in air. This takes only a minute or so. Fix in methanol. Stain following the usual process for your chosen staining. Wash thoroughly in distilled water. Let it dry in air. Wash three times in isopro...

Milou wrote: ↑

Mon Jan 09, 2023 6:43 pm

And I think it would be necessary to make a kind of adapter to put in place of the long black original lamp support?

Yes, and you need a proper power unit for the LED. The original incumbent bulb was fed with AC power, not suitable for any LED.

Try https://www.ebay.com/itm/255490368747.

I never used something from this seller. The risk is all yours.

To work properly your hemacytometer needs its special cover glass. It fits the chamber, it is grounded flat for precision and it is stable enough not to be bent by the capillary force in the filled chamber. All those properties are needed to have a defined volume in the chamber. A standard cover gla...

It is not mad, it is just a click-bait.

I don't see any safety issue with it but the results will not satisfy you. The green yellow and red light coming from the Mercury burner will be much stronger than any fluorescence light. As a result you will see no autofluorescence at all. There are filter cubes that allow only UV or UV + violett o...

I would like to know then what exposure times can freeze most motion. With this limitation in mind, I can only feasibly go to an exposure time of 1/250 sec with my camera. 1/250 is the fastest speed that opens the shutter fully on the 60D. Shorter times do not open the shutter fully which results i...

If you are based in India, give them a try. The "American" microscopes from Amscope et. al. are all made in China and not different from those Chinese brands. If they are good for you, there is a very good chance an Indian one is as well.

The 100x are immersion objectives. They are very tolerant against scratches on the front lens because the oil fill them op and there is extremely little refraction between glass and oil.

I thought Zeiss is more expensive, just realized one can make an account on their page and see the prices. The trinocular 305 with a base and gooseneck LEDs costs 2800€, that should be good, although a bit over the budget. https://pulchlorenz.de/mikroskope/stereomikroskope/stemi-305/stereomikroskop...