MicrobeHunter.com Microscopy Forum

Hi John,
the eyepieces are Zeiss Jena, P is non-correcting.
The thing might be part of a monocular tube or a drawing atattchment.
These Tessar lenses - do they contain a diaphragm and do they have a mounting- or focussing thread?
I don't think they were from a Werra.

Bob

Hi Doron, the lamp will probably be as little powerful in the Phomi as in your GFL. The Phomi has more mass as a heatsink though. I will have to see what I do. The "ABTO" control unit, named from the letters on the buttons, would easily house an additional LED-power supply too. The newer Phomi and U...

As far as I know only a few Phomi I were made in all-black. Most had a black direction changer head and bino tube and a gray hammerite arm and base and control box. I usually prefer all black microscopes but with the Phomi I'm not sure about this. Right now I'm working on the radiator and furnitue o...

Hi together, yesterday evening I lifted a Photomikroskop I onto my desk! I started to check the components and maintenance status and spent some time observing a stained plant section. It has seen little use over its 60 years and is in nice cosmetic condition. The Optovar with phase telescope is rea...

[quote="desertrat"] even a little uncomfortable for me to view with an eyepiece. So I think the eyepiece camera is getting plenty of light./quote] Hi Rick, I'm sure that your camera gets enough light then to make a good image. I would say when it starts to get a bit dim for the eye the camera might ...

Hi Doron, your USB camera does really well and I'm sure you will have fun with it. For web resolution and documentary work the very best camera solutions are a massive overkill and much of their capability doesn't show. On your GFL the USB cam does astonishingly well in web resolution but I'm sure t...

Tere is a good free stacking programm: Picolay.

Hi Rick, I'm no image editing expert myself but as far as I know usharp masking should be carried out once as the very last step of image editing and setting the final image size. From your three images I find the second on just fine - good aparent sharpness and little artifacts. When this would be ...

I had a look how he made these photos: - Zeiss West infinity microscope with Plan-Neofluar objectives - 4 Megapixel compact camera :lol: which tricks did he use: - preparation (embedded in slightly Fuchsin stained glycerin gelantine, using the refractive index difference to enhance details) - stacki...

Hi Peter, when I look at the second photo I would say that nothing is really in focus. Also there is no white in the image - is the background this light brown colour? The image also might be a bit on the contrasty but low resolution side - how was your condenser set? Here is a pollen image, taken 7...

Just in case someone looks critical at his old brass microscope now: The value of old brass microscopes suffers if you polish it up to a nice antique-trader-shine. The old microscopes were painted with a stained shellac specific for this maker and they are far more valuable if parts of this old pain...

The right amount of sharpening depends on the final size of the image: If you sharpen it right in the original size it will be too little after reducing the size for the forum. This is also a matter of taste but I would increase the amout of unsharp masking and accept a little grain for a crisper lo...

Hi Rick, these images are quite nice in fact. The only things I don't like are the banding and a slight lack of apparent sharpness. Do you have an idea where the banding comes from? The light source or the camera? Does changing the brightness of the lamp influence the banding? Do you use unsharp mas...

The condenser should support the objectives you use. In most cases the condenser is stopped down slightly below the aperture of the objective. If you have a 40:1 n.a. 0,65 objective you would use it with a condenser stopped down to ca. 0,5 to 0,65. When you remove an eyepiece and look into the tube ...

Hi Peter, nice to see that you've got the necessary pieces together. The images show some room for improvement but the important things work. These MF-Projektive were made in different versions all meant for direct projection on film of different sizes like 35mm, medium format 4x5 inch etc.. I don't...

Bob - tangential topic; do you happen to know where Leitz or Lomo would be on the correction scale? And did Zeiss change? We are told, (mostly be ebay sellers!) that Lomo = Zeiss. Hi Chris, post war: Zeiss West Standard: highest amount of built-in colour error, equal for (nearly) all objectives Zei...

Finally, a Zeiss 25X0.45 Ph2, not Plan, not Neofluar, performed quite well ! @Bob - I think that this is what you expected ? Hi Doron, One of the novelties of the Zeiss Standard system was that the color error of the stronger objectives was artificially calculated into the weaker objectives too. Th...

Hi Rick, the new layout comes especially handy when you want to print the book. The scanned original would be printed with the gray paper background making it toner-consuming to print and difficult to read. The original book is als quite small and re-formatted to A4 format the number of pages is red...

Hi Doron, thank you for reviewing this camera. There are often questions for a recommendation for a simple camera solution and this will probably do well enough for web resolution and documentary purposes. The object micrometer is a harsh test. In a normal image the colour error leads to a certain l...

If you don't like the Windows 8.1-on start menu you can buy a program called "Startisback" and have your Windows 7 start menu back. I have used it for 2 years now and never had a problem with Windows 8.1. It costs just 3$.

Bob

The nice aplanatic achromatic condenser is made for slides of 1,5 to 1,6 mm thickness. Todays slides have mostly 1,1mm. Apertures (here 1,4) over 1 can only be used effectively when the condenser is connected to the slide with a drop of immersion oil. This way you get homogenous oil immersion: Conde...

The colour difference might come from different amount or thickness of cemented surfaces but I think it is probably just the glass itself. Left and right have different light pathes and there is a lot of glass in a binocular tube. Glass can suffer from discolouration, but it is unlikely in this case...

Hi Tim, to me the difference between left and right seems to be about the same with both heads. So it might be typical for this model. Of cause a broken cement layer or the re-cementing could have an influence. I have had bino tubes with colour difference, but remember it to be less visible. Apart f...

To measure inside threads it is also a way to use a piece of wood or cardboard and transfer some paint or dirt marks of the thread onto it. Paper is not ideal as it deforms when pressed onto the thread. Bills cast method is more precise but usually I'm too much in a hurry!

Bob

Hi Doron,
Peter Höbel used near UV light to resolve Amphipleura p.:
http://www.mikroskopie-ph.de/index-Amphipleura.html

The upper left image shows about what is possible this way.

Bob

And, how do you know that I don't know much about designing ? The plastic and metal parts of a microscope is very simple to design. This sounds as if you were underestimating the demands of this design project by far. For a usable microscope you need fine tolerances and a high level of ridgidity - ...

It is possible to design a microscope from a 3D-printed body and some metal parts. But using 3D-printing actually makes it very diffcult to come to an acceptable design. And this is where the problem lies: The question you ask show that you don't know much about designing things and nothing about mi...

Van Heurcks flint glass slides and cover slips (Not to mention his arsenic based "yellow medium") would be difficult fo buy today. I'm sure that many of them have been thrown away because their value wasn't obvious. The same will have happend to most Nobert-test-plates. :evil: But imagine 1891: A bi...

Hi Rick, thank you for your documentation of how to get this thing resolved! I once got a bottle of water from the "Kraterquelle Bad Nenndorf", full of Amphipleura pellucida. In the end my solution was similar to yours, a decentred cardioid condenser did it, even with a humble n.a. 1,25 achromat. I ...

Hi together, perhaps this bit of information can help: The first generation of the Tamron 90mm 1:2,5 Macro lenses had a blue spot problem when used on a DSLR. Reason was the completely flat rear element that didn't work well together with the flat sensor. The problem became only visible when the ape...