Search found 761 matches
- Thu Jun 30, 2022 7:39 pm
- Forum: Microscopes and optics
- Topic: Assembling iris
- Replies: 18
- Views: 2817
Re: Assembling iris
Dudes are useless with the fine motor skills. I mean, they'll do it once, brag about it, then move on to the next thing. But no way they'll do it over and over again, correctly, and quickly, day in and day out. I agree with this guy 100% Ha, I've done it once, and talked about it many times... :oops:
- Wed Jun 29, 2022 6:29 am
- Forum: Illumination Techniques
- Topic: oblique illumination
- Replies: 44
- Views: 9003
Re: oblique illumination
Macro_cosmos has posted a couple of nice videos showing different illumination settings with the Heine:
https://youtu.be/_tecNY-yJNY
https://youtu.be/zRAHPIoL7tI
https://youtu.be/_tecNY-yJNY
https://youtu.be/zRAHPIoL7tI
- Wed Jun 29, 2022 6:27 am
- Forum: Microscopes and optics
- Topic: experience with a plan 1x compound microscope objective ?
- Replies: 56
- Views: 8657
Re: experience with a plan 1x compound microscope objective ?
Thank you Viktor for interesting pictures This plan achromat 1.3x objective has a much larger WD than the one I've tried (Olympus plan 1x) .... Therefore it allows place for episcopic light Your ping pong ball diffuser is very efficient :-) Glad you found it useful. I raised it in the picture to sh...
- Tue Jun 28, 2022 8:11 pm
- Forum: Microscopes and optics
- Topic: experience with a plan 1x compound microscope objective ?
- Replies: 56
- Views: 8657
Re: experience with a plan 1x compound microscope objective ?
As I recently picked up an Olympus short-barrel plan achromat 1.3x NA 0.03 objective, I thought it was interesting to take a couple of shots for comparison. It looks like this: https://i.imgur.com/bBwHbkE.png Images are taken on My Vanox AH using a Canon 5DmarkII Full frame camera and a matching FK ...
- Sun Jun 26, 2022 2:57 pm
- Forum: Microscopes and optics
- Topic: experience with a plan 1x compound microscope objective ?
- Replies: 56
- Views: 8657
Re: experience with a plan 1x compound microscope objective ?
Somewhat counterintuitively, low NA low magnification objectives have a greater depth of field at the subject plane, but a shallower depth of focus at the image plane. High-NA objectives are the reverse, with razor thin depth of field at the subject, and a very great depth of focus at the image side...
- Sat Jun 25, 2022 4:09 pm
- Forum: Camera systems and imaging
- Topic: Nikon Live view vs actual picture
- Replies: 8
- Views: 1671
Re: Nikon Live view vs actual picture
From what I've gleaned, most Nikon DSLR bodies don't have a true silent mode (EFSC or full electronic shutter). The ones I know that has this feature are their higher end full frame bodies. That's the main reason why so many have favored Canon DSLRs for photomicrography and photomacrography, where s...
- Thu Jun 23, 2022 8:13 am
- Forum: Illumination Techniques
- Topic: oblique illumination
- Replies: 44
- Views: 9003
Re: oblique illumination
Looking really good!
- Thu Jun 23, 2022 8:12 am
- Forum: Microscopes and optics
- Topic: experience with a plan 1x compound microscope objective ?
- Replies: 56
- Views: 8657
Re: experience with a plan 1x compound microscope objective ?
Thanks for sharing, the 1x looks horrible. But, to be fair...isn't the microscope objective corrected for transmitted light, and the macro for reflected, which is what this subject is ? Can't see how that would matter in this case. At such low NA, presence or absence of cover slip doesn't matter. L...
- Wed Jun 22, 2022 9:11 pm
- Forum: Microscopes and optics
- Topic: experience with a plan 1x compound microscope objective ?
- Replies: 56
- Views: 8657
Re: experience with a plan 1x compound microscope objective ?
Thanks for sharing, the 1x looks horrible.
- Wed Jun 22, 2022 6:08 pm
- Forum: My microscope
- Topic: 1961 Petrographic Microscope Leitz Laborlux Pol
- Replies: 7
- Views: 2010
- Mon Jun 20, 2022 2:14 pm
- Forum: Microscopes and optics
- Topic: Zeiss axiolab RE
- Replies: 14
- Views: 2105
Re: Zeiss axiolab RE
If you are seriously interested in microscopy and photomicrography, but has limited funding, I'd much rather spend my money on a 160mm Zeiss microscope, as these can reasonably affordably be equipped with a trinocular head, DIC, and fluorite/planapo objectives. With some careful shopping, you really...
- Mon Jun 06, 2022 11:40 am
- Forum: Beginner's corner
- Topic: Parfocalling-do it yourself?
- Replies: 12
- Views: 2624
Re: Parfocalling-do it yourself?
It's a spam bot.GerryR wrote: ↑Mon Jun 06, 2022 10:41 amPlease clarify; who explained it properly and what do people get wrong about it? Much appreciated. Thanks.haileynelson10 wrote: ↑Mon Jun 06, 2022 9:25 amParfocalling is exactly what you have explained. Some people sometimes are still wrong about it.
- Mon Jun 06, 2022 9:27 am
- Forum: For forum members who want to buy and sell equipment
- Topic: Wanted Camera attachment
- Replies: 2
- Views: 1340
Re: Wanted Camera attachment
I'd be very sceptical of buying something like this from a new member with three comments, all trying to sell hard-to-find items.
Seems very unlikely that someone would have a spare Zeiss Axiomat 50x PlanApo, a 30mm phase telescope, and a Russian MBC-10 camera attachment...
Seems very unlikely that someone would have a spare Zeiss Axiomat 50x PlanApo, a 30mm phase telescope, and a Russian MBC-10 camera attachment...
- Mon Jun 06, 2022 9:27 am
- Forum: For forum members who want to buy and sell equipment
- Topic: WTB/ barrow 30mm Centering telescope lens
- Replies: 8
- Views: 2077
Re: WTB/ barrow 30mm Centering telescope lens
I'd be very sceptical of buying something like this from a new member with three comments, all trying to sell hard-to-find items.
Seems very unlikely that someone would have a spare Zeiss Axiomat 50x PlanApo, a 30mm phase telescope, and a Russian MBC-10 camera attachment...
Seems very unlikely that someone would have a spare Zeiss Axiomat 50x PlanApo, a 30mm phase telescope, and a Russian MBC-10 camera attachment...
- Mon Jun 06, 2022 9:26 am
- Forum: For forum members who want to buy and sell equipment
- Topic: Wanted: Zeiss Axiomat - Planapo 50x 0.95 with collar 0.14-0.22
- Replies: 16
- Views: 4518
Re: Wanted: Zeiss Axiomat - Planapo 50x 0.95 with collar 0.14-0.22
I'd be very sceptical of buying something like this from a new member with three comments, all trying to sell hard-to-find items.
Seems very unlikely that someone would have a spare Zeiss Axiomat 50x PlanApo, a 30mm phase telescope, and a Russian MBC-10 camera attachment...
Seems very unlikely that someone would have a spare Zeiss Axiomat 50x PlanApo, a 30mm phase telescope, and a Russian MBC-10 camera attachment...
- Wed Jun 01, 2022 5:49 pm
- Forum: Illumination Techniques
- Topic: LED Illumination Comparison
- Replies: 50
- Views: 7949
Re: LED Illumination Comparison
On my Canon 5D mark II, I usually take a photo of a blank slide after setting up Kohler in brightfield, and then use the photo to set a custom white balance profile. I find it very easy and reliable.
- Wed Jun 01, 2022 12:18 pm
- Forum: Microscopes and optics
- Topic: Zeiss water immersion objective front lens damage
- Replies: 28
- Views: 4239
Re: Zeiss water immersion objective front lens damage
On one objective, I had a really stubborn layer of dried up immersion oil. It literally took a hundred q-tips with various solvents before I got everything off. And it took quite many attempts before I started to be able to get it loosened up. So it's worth trying a bit more with different solvents ...
- Fri May 27, 2022 4:21 am
- Forum: Identification help
- Topic: Egg deposit? Cocoon thingie?
- Replies: 1
- Views: 737
Re: Egg deposit? Cocoon thingie?
Chironomidae eggs would be my guess.
- Fri May 27, 2022 4:18 am
- Forum: Illumination Techniques
- Topic: LED lighting....why the hate?
- Replies: 72
- Views: 14385
Re: LED lighting....why the hate?
Well, I was wrong! That's a very impressive spectrum for a single emitter. Would be interesting to know what model it is. I agree that the light flux per chip could be a problem, though. But very cool to see such a high quality spectrum on a cheap consumer led product. Is your plan to desolder the c...
- Thu May 26, 2022 4:37 pm
- Forum: Illumination Techniques
- Topic: LED lighting....why the hate?
- Replies: 72
- Views: 14385
Re: LED lighting....why the hate?
Nice spectrum, but certainly that spectrum is not produced by a single die, or even a single COB led. It is probably produced by a combination of many different LED dies. For microscopy, we need a single die, or a fairly small COB led. The Yuji and Optosolis COB leds are the closest thing that I've ...
- Thu May 26, 2022 8:54 am
- Forum: Illumination Techniques
- Topic: oblique illumination
- Replies: 44
- Views: 9003
Re: oblique illumination
Looks good!
- Thu May 26, 2022 5:18 am
- Forum: Illumination Techniques
- Topic: oblique illumination
- Replies: 44
- Views: 9003
Re: oblique illumination
To me, the U-shaped wrap around solution seems like the only viable option: https://i.imgur.com/gYfWw3r.jpg If I were you, I'd draw it up in Fusion 360 and 3D print it. If that doesn't turn out sufficiently rigid and properly aligned, it would probably need to be machined on a mill. But I think the ...
- Tue May 24, 2022 6:46 pm
- Forum: Illumination Techniques
- Topic: LED lighting....why the hate?
- Replies: 72
- Views: 14385
Re: LED lighting....why the hate?
Professional led systems use as complete a combination of spectral lines as is possible in order to attempt to cover the similar but more gradual transition across spectra that filament lamps provide in order to produce as contrasty and high resolution imaging as is possible. The LEDs in the profes...
- Sat Apr 30, 2022 8:37 am
- Forum: Microscopes and optics
- Topic: How to differentiate a PLAN FLUOR lens?
- Replies: 2
- Views: 952
Re: How to differentiate a PLAN FLUOR lens?
Specific brand in mind? Fluorites almost always have higher NA than corresponding achromats. And will be labeled FL, Fl, Fluor, Fluotar, Fluar or something like that. Though some brands made a class of better corrected achromats that have slightly higher NAs and likely had at least some low dispersi...
- Mon Apr 25, 2022 5:31 pm
- Forum: Microscopes and optics
- Topic: Olympus dic on optiphot
- Replies: 10
- Views: 2136
Re: Olympus dic on optiphot
Might be tricky. Even if you are mechanically able to attach the Olympus intermediate piece to the stand, you are likely going to change the prism-to-objective distance, which can be pretty bad for DIC. But then again, if you are going to use it with mismatched Nikon objectives, there's a slight cha...
- Mon Apr 25, 2022 4:10 am
- Forum: Microscopes and optics
- Topic: How to diassamble Wild M20 binocular head?
- Replies: 7
- Views: 1757
Re: How to diassamble Wild M20 binocular head?
I normally begin with Zeiss lens cleaner, and finish with isopropyl alcohol. I believe that's what I did. Getting the prisms perfectly clean from edge to edge takes a little patience.
- Sun Apr 24, 2022 6:32 pm
- Forum: Microscopes and optics
- Topic: How to diassamble Wild M20 binocular head?
- Replies: 7
- Views: 1757
Re: How to diassamble Wild M20 binocular head?
http://www.microscopy-uk.org.uk/mag/art ... sbino.html
This article should give you the information you need. It helped me when I had to clean a foggy M20 binocular head.
This article should give you the information you need. It helped me when I had to clean a foggy M20 binocular head.
- Sun Apr 24, 2022 4:57 pm
- Forum: Microscopes and optics
- Topic: Nikon Interference Phase Contrast (IPC)
- Replies: 37
- Views: 8782
Re: Nikon Interference Phase Contrast (IPC)
Another option is to print a bunch of concentric rings of known dimension on transparent paper and put it where it is supposed to go, then look at it with a phase telescope to see which size is right.
- Fri Apr 15, 2022 5:18 am
- Forum: Pictures and Videos
- Topic: Diatom - 313nm transmission image
- Replies: 51
- Views: 8555
Re: Diatom - 313nm transmission image
Impressive results, Jonathan, both the diatom and resolution target. As you know, I've been following this project with great intrest and admiration. Noticed that you are using a 0.85 condenser. I assume that high-NA quartz condensers are few and far between. Have you though about using a high-NA UV...
- Fri Apr 15, 2022 3:45 am
- Forum: For forum members who want to buy and sell equipment
- Topic: Wanted: Olympus BH2 DIC
- Replies: 24
- Views: 5244
Re: Wanted: Olympus BH2 DIC
I found this prism. Is it the one you use? Yep, those are the ones I have. Remaining questions: 1. Condenser dovetail 2、SPLAN LB..... Thank you very much. 1. You will need to DIY a dovetail like the one on PeteM's condenser. 2. Can't say how they'll work, I don't have access to any SPlans, unfortun...