MicrobeHunter.com Microscopy Forum

Hobbyst46 wrote: ↑

Fri Jul 29, 2022 2:32 pm

Amazing images !

And very good use of the 40x0.95.

Thank you Hobbyst

Hi Adam, Thank you for your comment. The diatom measures at 40x60 µm. I used DIC with a 40/0,95 apochromat Zeiss Jena objective, the microscope was setup for afocal projection with a 10/18 Kpl-W eyepiece (with a 40 mm pancake lens on a Canon 50D). The diatom was attached to the underside of the cov...

I would like to share a small series of optical z-sections of an Amphora diatom that had attached to the underside of a floating coverslip. I decided not to stack as in this series of sections you can see different organelles such as the nucleus and chloroplasts in positions relative to each other. ...

Quite a nice finding and superb documentation. Btw you can directly embed the video here in the thread by placing the video ID within the youtube tags

e.g. the ID of your video is HaGDw2e6cBg and you put it [*youtube ]*here*[/youtube*] (without the asterisks)

Nice! Post a picture of it if you can.

Very good quality, what microscope/objective and camera did you use?

Crusty wrote: ↑

Sat Jul 02, 2022 11:31 pm

Really nice. Which objective did you use?

tlansing wrote: ↑

Sat Jul 02, 2022 3:47 pm

The microscope is a Zeiss Photomicroscope III, 16x Neofluar objective, 1.25x optovar, DIC, flash.

Fascinating observation, Tim. Thanks for posting.

Sure Squintsalot wrote: ↑

Wed Jun 29, 2022 10:11 pm

Dudes are useless with the fine motor skills.

I mean, they'll do it once, brag about it, then move on to the next thing. But no way they'll do it over and over again, correctly, and quickly, day in and day out.

I agree with this guy 100%

I can recommend acridine orange, as previously mentioned its very cheap and emits in multiple parts of spectrum depending on local conditions such as pH. I used to have a small collection of GFP-transgenic Arabidopsis seeds that gave rise to plants expressing GFP in different compartments (sadly I l...

Intriguing finding and very well documented at that. I am more inclined to think these are predatory/parasitic bacteria. Maybe Vampirococcus?

was to buy an air purifier. Basically a high grade HEPA filter with a fan that sucks in air and releases completely dust-free air back into your microscopy dedicated area. Not having to deal with dust getting on and in the microscope has been such an improvement and I highly recommend to anyone stru...

Where is 75RR??

It makes you wonder if at some point the bots will get so good they actually start providing useful contributions! Maybe they are already doing that... :shock: Have you seen what some of the AI is capable of today? Sometimes I legitimately wonder what % of online interactions are with real people.

Very nice finding Javier. Can you tell me more about its habitat, what kind of pond was it, did you scrape any pond scum, slime, decaying plant matter or similar? Did you take the sample from a shady place or a sunlit area? I have never seen Lacrymaria in the wild. I cannot say I'm happy with the qu...

I had no idea but I have seen it on other forums. Maybe the question is too easy? The problem is that the tech is getting better; it's pretty trivial these days to programatically parse a simple question and get the answer. At the point you start tripping up the program, humans tend to be failing a...

Ok so what solution do you offer?

I like how clearly you can show the ejectosomes.

This is an old issues, yes. I raised it some years ago https://www.microbehunter.com/microscopy-forum/viewtopic.php?f=8&t=8276 What I suggest is to have a question at the time one registers. Something simple yet relevant to the hobby for example "Which part of the microscope do you look through wit...

LouiseScot wrote: ↑

Fri May 27, 2022 7:01 pm

Plus maybe 'why do you want to join this forum?'

yeah but that can't be easily automated. You want a filter that doesn't require the conscious attention of the admin.

This is an old issues, yes. I raised it some years ago https://www.microbehunter.com/microscopy-forum/viewtopic.php?f=8&t=8276 What I suggest is to have a question at the time one registers. Something simple yet relevant to the hobby for example "Which part of the microscope do you look through with...

I need to develop an application in a short time, but I do not have enough specialists to successfully complete all tasks. I am interested in outstaffing services. What are the terms of IT outstaffing as a service? Entirely unrelated to anything this forum does. Kindly ask your programmer to tweak ...

Thank you Tim and Adam Great stuff Wes. The 25x Planapo is my favourite objective - do you have the dedicated DIC slider or are you using a different one? I also like it a lot, it provides a great compromise between magnification, resolution and spherical aberration. I use the slider for the 6,3x pl...

Thank you Javier and Greg.

Javier wrote: ↑

Tue May 17, 2022 9:44 pm

Nice catch and wonderful video, Wes!

The detail of the armour is great. I also liked a lot the big Paramecium. Why is it so shiny? Did you use also polarized illumination?

I use DIC which relies on polarized light.

Coleps is a genus of predatory ciliates that feed on bacteria, algae, other protists (dead and alive) as well as animal and plant tissues. Here you can see one Coleps specimen feeding on what I believe is a rotifer egg. If you look closely you can follow the path of egg particles making their way to...

Beautiful!

Life and work can really get in the way of enjoying hobbies I know that feeling all too well Therefore, my microscope can be advertised as 12.5X - 500X capable. I rarely go above 500x. Really looking forward to see some of the shots you produce with this fine looking scope Can you give us a rough i...

This microscope comes with an achromat-aplanatic BF-Ph-DF condenser type V Z. So to answer your question, no the condenser does not have DIC function (only phase and darkfield). Cosmetically the microscope looks very nice, and assuming internally there is no delamination and other optics issue this ...

Can I have a free sample for my lab? I will write a review.

Yeah, there are some impressive videos on YT of huge amoebas capturing different ciliates like you describe. It's brutal! I am more into botany, but sometimes I like to watch these microorganisms living in pond water etc. Unfortunately I rarely found amoeba. In which environment are they most likel...