MicrobeHunter.com Microscopy Forum

Saul wrote: ↑

Wed May 06, 2020 11:06 pm

It will be mostly Mitutoyo set, Nikon BD infinity set, some Olympus/Nikon high NA

Do you have a ring mirror to widen out the Nikon BD lighting to the 40mm Mitutoyo BD lighting?

So this looks like a filter for a DMIL lamp. What would a full lambda waveplate inserted right after the light source?

https://www.ebay.com/itm/Leica-Leitz-We ... 3435021491

Could you give dimensions and use a collimated beam of light to find the focal length for red, green, and blue? I'm curious what the corrections might be. But if there is no big frequency difference, then maybe a surplus doublet can be found. Is it planar convex? Or is it a negative doublet?

If you just want to experiment, just buy this set of 10 square films for $8: https://www.amazon.com/Polarizing-Film-Sheet-Gadget-Electronics/dp/B004X3XFHU/ref=sr_1_4?dchild=1&keywords=polarizer+film&qid=1591897183&sr=8-4 If you want what the microscopes use then these from Edmund Optics go for a muc...

It's interesting that the Internet will not share information until the right key words are found: https://caeonline.com/buy/mask-wafer-in ... -200/77516

Thanks.

Cool. Found a Leitz Ergoplan that had been sold here with picture: https://caeonline.com/buy/microscopes/l ... plan/52994

I guess I don't understand, the beam splitter mirror is round? It's not elliptical. Unitron's had small mirrors but they are elongated, because of the 45 degree slant. But this older Zeiss it's round?

https://www.wired.com/story/amphibians-glow "This kind of biofluorescence only happens when blue light hits the amphibian, whose skin—and bones, in the case of the marbled salamander—absorb that wavelength and emit a different wavelength, usually an electric green. (This is not the same as biolumine...

When I took these images I probably was not using an NFK relay lens -- I'm not certain. It's not in my notes. Are you using an NFK in your set-up? There are corrections done for these New(D/S)Plan objectives. But I doubt that would fix anything. Still it's something to ask about, especially since I ...

Here are two more 10x NeoDPlan, with and without DIC. With is less satisfying. Side note: The purple tint in the middle of these two images is not from the Olympus set up. The blobs are from the USB camera having the Fuji defect where the green pixels on the sensor are "lensed" to get light from a r...

Here is a post where I hooked up a B&L DIC filter adapter. (http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=5&t=7327) Attached pictures here, shown below, have two not published in the B&L thread that are from the 10x NeoDPlan with and without DIC for comparison. I do see blurring on t...

So here is a objective turret I had tested with DIC. Now looking it up to find your NeoSPlan objectives, I find that the NeoDPlan M26's shown here are not checked marked for DIC like your NeoSPlans M26's. Didn't know that. They seem to work. All these require NFK relay lenses to correct for color, b...

Hi, Does someone have dimensions on the DIN slot that goes through a U-TAD slider? I don't have that part, but would like to know about its cut-away path through the main body, and the position of the holder screw, and the slot below for those 6x20 DIN slide that have a stop screw/peg on the bottom....

Really cool.

Would be great to see the steps taken, and the recipe for the mount on the cover slip.

It is a good thought, but unfortunately I have tested it. Doubling only occurs when the dic prisms are in. I can get clean epi brightfield and darkfield. You said you had matched objectives to prisms. But out curiosity: what objectives are you using? I'm assuming these are the flip in-and-out turre...

Putting this on my to-do and understand list: If you look in an Axiomat, it has to be putting a metre of light path between the objective and the tube lens. It makes one wonder what the implications are. On the other side, Mitutoyo objectives always say 200mm TL, but work fine on an Olympus with a 1...

Curious to see these:

Single-Material Aspheric Achromats

https://www.edmundoptics.com/knowledge- ... -achromats

Any such video would probably involve some focusing, so feature points will be at different layers of a specimen slide. Maybe as the video runs, one section is filmed, that previous data can match up feature points to the next exposed section of the slide as it racks focus again. But you'll have fea...

You don't have to lift it, just look through it :lol: . and a basement to use it in so it doesn't fall through the floor. :lol: :lol: :lol: But it's an Axiomat! They do everything -- plus levitate. I mean, if you can find that attachment. It doesn't show up on e-bay that often. But once you get it,...

Cool. I learn a lot from the forum.

Thanks.

Erm, wouldn't Thorlabs confocal system be a laser scanning confocal?? That's different from a spinning disk confocal. Yes it is scanning, but it has a pin hole -- just a single one instead of a spinning disk's array of them. So I'm using the Thorlabs scanning set-up as an example of a pin hole came...

I have a Leica Leitz DMIL inverted that takes 160mm objectives. I also have 10x 160/- PHACO objectives, plus 4x and 10x 160/- objectives. I could test PHACO 2 and 3 with 160/.017 25/0.50 and 160/.017 100/01.25 objectives, but with the 0.17 cover slip thickness are these actually used in inverted set...

I'm liking the idea of augmenting at the eyepiece image plane. I could add Hoffman modulating contrast to a Leica DMIL 160mm objective microscope by extending the neck below the trinocular. I guess one could even add a small epi there.

Well... in a infinite system with a spinning disk confocal setup you should have the pinhole disk "after" the tube lens (or secondary objective, in Nikon jargon) That's why I'm asking, because Thorlabs does it above the tube lens, which I can follow the design of. https://www.thorlabs.com/newgroupp...

BTW, Nikon does it like this in their "External Phase Contrast" system on their inverted microscopes. IMG_1642.jpg So for the Nikon assembly, would a series of two Steinheil triplet achromatic lenses near the objective side, plus a Hastings to feed into the tube lens side be a good optimal set up? ...

The BFP is the Fourier plane of the objective :-) So if the BFP is at the left of L1, it will be relayed to the right of L2. The 4f relay is normally used to Fourier transform the "image" made by the objective by L1, and do a following inverse Fourier transform with L2. If you're relaying the BFP w...

"I'm curious, why use the U-CPA unit? You only have to plop the 4f unit above whatever trinocular you have. Plus you have to move L1 around to focus on the BFP, whereas using the normal primary image plane through a trinocular lets you have a solid setup with no fiddling about." I'm just trying to u...

The image of the BFP would be f2 right of l2.

But the Fourier plane would be f1 right of l1, f2 left of l2. Right?

Thanks,
Ted

Thanks.

I had not thought about low resolution contrast.