Search found 4277 matches

by Hobbyst46
Tue Jan 02, 2024 9:33 pm
Forum: Home-made microscope adaptations
Topic: Dichromatic Illumination?
Replies: 61
Views: 115429

Re: Dichromatic Illumination?

… I think, still, that the OPs project will still offer some interesting results … Most definitely … I’m looking forward to seeing them MichaelG. Gee fellows, I don't know if there will be any interesting results. I am a complete beginner in microscopy and a lot of the stuff you guys talk about go ...
by Hobbyst46
Fri Dec 29, 2023 9:50 am
Forum: About the website, the magazine and this forum
Topic: New trouble uploading images...
Replies: 19
Views: 16164

Re: New trouble uploading images...

Same issue here. Cannot upload photos.
by Hobbyst46
Wed Dec 27, 2023 7:10 pm
Forum: Miscellaneous
Topic: Snow.
Replies: 5
Views: 4629

Re: Snow.

Thanks for pointing out this fascinating subject!
by Hobbyst46
Wed Dec 27, 2023 11:47 am
Forum: Home-made microscope adaptations
Topic: Dichromatic Illumination?
Replies: 61
Views: 115429

Re: Dichromatic Illumination?

It might help to know which type of microscope illumination is wished. Both brightfield and fluorescence, at least, benefit from a single die LED source, rather than a multi-LED arrangement. Besides, I wonder about the wavelength combination... For visual observation, green or blue or amber light ar...
by Hobbyst46
Tue Dec 26, 2023 3:51 pm
Forum: Beginner's corner
Topic: Olympus NIC/Phase Contrast condenser repair question
Replies: 8
Views: 4690

Re: Olympus NIC/Phase Contrast condenser repair question

I have an old condenser top lens, Olympus achromat aplanat 1.4, in fairly good condition I think. Looks similar to yours and perhaps will fit your condenser. Thread diameter 35mm. Top glass diameter 16.4mm. Glass looks clean, except for two tiny chirps (diameter, say <0.5mm) on the top glass. I beli...
by Hobbyst46
Wed Dec 20, 2023 10:33 pm
Forum: Beginner's corner
Topic: Tools for dissecting really tiny spiders and insects
Replies: 6
Views: 4042

Re: Tools for dissecting really tiny spiders and insects

You might try very fine syringe hypodermic needles. They go by gauge number, #33 is the finest I ever met, but #27 (and maybe grades #28-#30 too) are easy to find (on eBay, etc). The tip of a hypodermic needle is actually a tiny blade. Another useful very thin utensil, though not a blade, is the nee...
by Hobbyst46
Mon Dec 11, 2023 4:02 pm
Forum: Microscopes and optics
Topic: optiphot-1: fine focus knob trouble
Replies: 9
Views: 4952

Re: optiphot-1: fine focus knob trouble

There is a manual named:
Nikon OPTIPHOT BODY LABOPHOT REPAIR MANUAL
for the beginner and experienced technician alike (seriously!)
It has been downloadable from the University of Arizona site.
It provides exploded views and instructions for disassembly of the focusing gear.
by Hobbyst46
Wed Dec 06, 2023 1:17 pm
Forum: Microscopes and optics
Topic: Let’s talk about Delamination
Replies: 30
Views: 54614

Re: Let’s talk about Delamination

iPeace wrote:
Wed Dec 06, 2023 6:53 am
The lens closest to the eye is a single lens. The lens furthest from the eye is a doublet.
Is it a KPL or KPL-W eyepiece ? your description of the issue does indeed indicate delamination.
by Hobbyst46
Sat Dec 02, 2023 8:48 pm
Forum: Microscopy accessories
Topic: Cleaning excitation filtres
Replies: 13
Views: 11159

Re: Cleaning excitation filtres

I can't get any light to come through.... I noticed the back of the cube is labeled "Omega XF06." This describes the following setup: Exciter: XF1005 (365WB50) Dichroic: XF2001 (400DCLP) Emitter: XF3002 (450DF65) as described here: http://www.glenspectra.co.uk/glen/filters/pdfs/XF06.pdf But the Oly...
by Hobbyst46
Sat Dec 02, 2023 7:01 pm
Forum: Microscopy accessories
Topic: Cleaning excitation filtres
Replies: 13
Views: 11159

Re: Cleaning excitation filtres

Perhaps I am utterly stupid but could it be that there are actually two glass filters, in two separate metal holders in series ? One holder contains nice violet-reflecting glass, probably the BP545 filter. It passes green and reflects violet. The other holder contains what looks like a green-reflec...
by Hobbyst46
Sat Dec 02, 2023 4:10 pm
Forum: Microscopy accessories
Topic: Cleaning excitation filtres
Replies: 13
Views: 11159

Re: Cleaning excitation filtres

Perhaps I am utterly stupid but could it be that there are actually two glass filters, in two separate metal holders in series ? One holder contains nice violet-reflecting glass, probably the BP545 filter. It passes green and reflects violet. The other holder contains what looks like a green-reflect...
by Hobbyst46
Sat Dec 02, 2023 1:26 pm
Forum: Microscopy accessories
Topic: Cleaning excitation filtres
Replies: 13
Views: 11159

Re: Cleaning excitation filtres

I am not on track with uploading images, but I think this link will allow you to see pictures of the Olympus BH2 G cube, and its opaque inner surface of the BP545 excitation filtre. It is blocking absolutely all light projected to it. The dichroic mirror and emission filtre seem good. https://photo...
by Hobbyst46
Sat Dec 02, 2023 11:43 am
Forum: Camera systems and imaging
Topic: Using Industrial Microscope Camera having panasonic CMOS sensor.
Replies: 4
Views: 4528

Re: Using Industrial Microscope Camera having panasonic CMOS sensor.

Likely, full control of the camera is only available when it is connected to the HDMI. I wonder about untold limitations in the declared simultaneity of output to HDMI and USB. Also, USB 2 is inadequate for decent videos. Also, the pushbuttons are small and dense and are possibly a source of vibrati...
by Hobbyst46
Fri Dec 01, 2023 10:40 pm
Forum: Microscopy accessories
Topic: Cleaning excitation filtres
Replies: 13
Views: 11159

Re: Cleaning excitation filtres

Thanks to everyone who has contributed to my many questions about epifluorescense. Being new to this modality, new issues keep coming up. One pf my filtre cubes has an excitation filtre that has accumulated something on the internal surface, to a degree where it is completely opaque, and no light i...
by Hobbyst46
Wed Nov 29, 2023 11:44 am
Forum: My microscope
Topic: reichert jung microstar 110 fluorescence
Replies: 2
Views: 3893

Re: reichert jung microstar 110 fluorescence

I can do blue excitation light. But i would like to see the red autoflorescence of vegetal stuff. I use a RGB LED torch that works wonderfully but i have two diferent position for the cube filter and i think that none of them allows for that. Should i try to change the filters in the cube? I think ...
by Hobbyst46
Tue Nov 28, 2023 12:45 pm
Forum: Illumination Techniques
Topic: Darkfield diagnostic test slide
Replies: 26
Views: 68232

Re: Darkfield diagnostic test slide

Perhaps a more or less uniform layer of small opaque particles of a defined diameter can serve for that purpose. Polystyrene spheres and other polymer particles are available from lab suppliers as suspensions that can be diluted with water as needed. A dried drop on the coverslip will make the test ...
by Hobbyst46
Sat Nov 25, 2023 9:17 pm
Forum: Beginner's corner
Topic: How to connect a microscope to a monitor
Replies: 6
Views: 4365

Re: How to connect a microscope to a monitor

Also note that the listed camera lacks a 0.5X reducing lens, which likely will provide a much wider FOV than without it, and that the FPS is relatively low at high quality video. I would prefer USB 3 rather than USB 2, if choosing an eyepiece camera. The listed camera appears to be bundled with a co...
by Hobbyst46
Fri Nov 24, 2023 12:27 pm
Forum: Microscopy accessories
Topic: BH-2 RFCA, filtre cubes, filtres and accessories
Replies: 10
Views: 6376

Re: BH-2 RFCA, filtre cubes, filtres and accessories

2) I understand what you write - but why is it in the analyzer slider? Any particular reason? What's the benefit of this, in lieu of placing it in the cube itself? Are an analyzer and cube installed together, such that the rays pass through both of them in series ? in that case, perhaps the previou...
by Hobbyst46
Thu Nov 23, 2023 9:54 am
Forum: Microscopy accessories
Topic: BH-2 RFCA, filtre cubes, filtres and accessories
Replies: 10
Views: 6376

Re: BH-2 RFCA, filtre cubes, filtres and accessories

Having had success in asking for help on lubricating the cube slider on my RFCA, I dare post a few more questions, as the epifluorescence modality and its various accessories are new to me - so here are my current inquiries: 1) What is the difference between the BH2-ANF and the analyzer dummy? I on...
by Hobbyst46
Wed Nov 22, 2023 4:28 pm
Forum: Illumination Techniques
Topic: DIY Fluorescence microscopy?
Replies: 16
Views: 11130

Re: DIY Fluorescence microscopy?

maguee wrote:
Sat Nov 18, 2023 7:37 am
Is there any way to repair a dicroic mirror?
If the coating is peeling off or has changed color, the mirror cannot be repaired.
by Hobbyst46
Fri Nov 17, 2023 9:27 pm
Forum: Microscopes and optics
Topic: Meiji ML2000L for $150 reasonable?
Replies: 11
Views: 4575

Re: Meiji ML2000L for $150 reasonable?

What's even more ridiculous is I have a degree in molecular cell biology which required doing all sorts of microscopy (including plenty of fluorescence microscopy on organisms I had to genetically modify), although in my defense I don't use that degree for anything, and it's been many years since I...
by Hobbyst46
Tue Nov 14, 2023 3:32 pm
Forum: Illumination Techniques
Topic: Help: has anyone ever used Olympus DCW Darkfield condenser?
Replies: 31
Views: 98436

Re: Help: has anyone ever used Olympus DCW Darkfield condenser?

osterport wrote:
Tue Nov 14, 2023 3:26 pm
As the last step, I swap to 100x Reichert 100x NA1.30+DCW+LED Ring, it's a mess.

We can see some details, but very poor quality
If the 100X Reichert has a fixed NA of 1.30, getting true DF will be (nearly) impossible. With most DF condensers, anyway.
by Hobbyst46
Tue Nov 14, 2023 3:29 pm
Forum: Illumination Techniques
Topic: Help: has anyone ever used Olympus DCW Darkfield condenser?
Replies: 31
Views: 98436

Re: Help: has anyone ever used Olympus DCW Darkfield condenser?

Thank you all so much for the reply! I'll take a picture tonight to show how messy it is. The most difficult part is, I can not use centering Telescope to properly center it, it's just dark, not like with other condensers where you can try different stops to see where the boundary is. BTW, when I t...
by Hobbyst46
Thu Nov 09, 2023 9:31 am
Forum: Illumination Techniques
Topic: autoflorescence
Replies: 9
Views: 6098

Re: autoflorescence

Fluorescence microscopy requires a strong illuminating (say, blue) beam and a VERY efficient long pass filter that passes ONLY the (say, green, yellow, red) weak fluorescence. Why would you be using a blue light, instead of a UV light? Not only would UV be easier to block, but wouldn't it also crea...
by Hobbyst46
Wed Nov 08, 2023 11:34 pm
Forum: Illumination Techniques
Topic: autoflorescence
Replies: 9
Views: 6098

Re: autoflorescence

Highly unlikely in a "standard" trans-illuminated microscope. Some internet sites have advocated sort of DIY epi-illumination arrangements on such microscopes, like a diagonal excitation LED beam (from above) combined with a long-pass filter on the eyepiece, etc. I am afraid that most of them are ov...
by Hobbyst46
Sat Nov 04, 2023 8:50 pm
Forum: Microscopes and optics
Topic: Zeiss Phase Contrast Condenser question
Replies: 6
Views: 2187

Re: Zeiss Phase Contrast Condenser question

When I disassembled the condenser a tiny metal ball fell out. I assume it is for indexing the turret because without it the turret turns freely. Unfortunately I don't see an obvious place for the ball, suggestions greatly appreciated. I am missing the knob and bracket shown in the last picture abov...
by Hobbyst46
Sat Nov 04, 2023 10:20 am
Forum: Illumination Techniques
Topic: Correct position of darkfield/oblique patch stop in relation to condenser?
Replies: 5
Views: 2113

Re: Correct position of darkfield/oblique patch stop in relation to condenser?

That sucks; its manual mentions filters... To be fair, it *does* come with filters, they just intend them to go here: ... The marked position is designed only for filters (e.g.polarizer) that completely pass the entire diameter of the illumination beam. The ideal place for a patch-stop is as close ...
by Hobbyst46
Wed Nov 01, 2023 3:02 pm
Forum: Microscopes and optics
Topic: Reassembly of the leaves of the field diaphragm of a Zeiss PM3
Replies: 4
Views: 1355

Re: Reassembly of the leaves of the field diaphragm of a Zeiss PM3

This may help some, maybe see #8 (or #18 ?) of the following previous post:
Re: Help needed - 1) Faulty phase contrast, 2) re-assembly of field diaphragm leaves Zeiss standards

Often questions about Zeiss are answered in detail on the German forum.