MicrobeHunter.com Microscopy Forum

Shame on me for not having the faintest idea what is that kit, and what exactly is its purpose.

I guess that the "water cell" you refer to serves for cooling. It is a glass flow cell, optically flat, so water flows between two parallel glass walls. It is connected in series with a water pump (peristaltic pump) and a reservoir. Rather than pure water, it is a solution of cupric sulfate, the blu...

Great photos. To my taste the DF is nicest...

Hello Tomecki, Note that the LED source from RetroDiode has been dimmed with a PWM. PWM is less suitable than a constant current power supply. PWM may cause dark bands in photo images. So if you plan to do photography, I suggest to inquire about their 21KHz PWM, it is better than the basic PWM. Also...

Thanks MichaelG for this link to an enjoyable article! In addition to the main theme, it is a reminder that great scientists are liable to be remembered on behalf of their mistakes, or heated controversies with contemporary scientists and colleagues, or anti-social behavior, not less than due to the...

Hi, concerning a simple vacuum device/chamber. I use a hand vacuum-pump designed for use with car brake systems, see my post HERE It may be just the job for a small chamber, although in the post I use it to degass plant tissue during processing for slide making. John. I have a similar vaccuum pump ...

[...] Also, if you consider doing photomicrography, then I believe that, if you purchase a camera that is fully remote-controlled by software, it might perhaps save you the search for reticles. Only an inexpensive (20-30 USD or less on eBay) stage micrometer will be required for calibration. How wo...

Great pictures. Thanks.

Thanks Bob! The images I posted are really quite raw. Single images, resized and slightly cropped to fit the upload limitations. I believe, though, that also Rod's diatom view 7 is not post processed... Incidentally, I just developed the simplest vacuum device, based on two plastic syringes in paral...

Welcome tomecki. I am using a LED lamp from RetroDiode.com with my (relatively) small microscope for more than a year by now. So I gained experience - mostly positive. I will gladly provide details, if you are interested. Also, if you consider doing photomicrography, then I believe that, if you purc...

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...I meant to say that Hobbyst46 should not expect his phase-NOA61 photos to be as dot-resolving as your brightfield-Pleurax photo, as many factors are different. Apparently, he already knew that and had a slightly different purpose (which was initial proof of concept) in mind. yes, indeed. I thoug...

When making arranged diatom slides on regularly spreads thin layers of e.g. shellac and skin fat on the cover slip to first deposit the diatoms and then glue them in place. With NOA61 you might be able to smear it onto the cover slip with a piece of plastic or so. One additional advatage would be t...

...In the meantime, I have started on the aperture revolver [Blendenrevolver]. It is the kind of disassembly and reassembly that one has to get right the first time! I wish you luck with this job. maybe this previous post will be of interest (the aperture part of the post): http://www.microbehunter...

...thinking about it even more, the colorimeter like you're thinking about takes two separate images and allows you to view them in a comparison eyepiece, side-by-side, for the sake of comparing the hue. In the setup pictured, the two images are slightly offset and converge in your brain, as a true...

Sorry, I do not see any pictures.

Thanks all folks for your kind responses! I am still working on it but would like to comment on as far as I know. My NOA61 slides also have these air bubbles in many of the larger diatoms. I have seen recommendations with other mountants to first wet the slide with a drop of solvent to displace the ...

Here is my experience with (hopefully) permanent strew slides of diatom mounted in the UV-curable adhesive NOA61. I brought it to room temperature prior to use. Dried a drop of an old batch of gently cleaned diatoms on coverslips. Then, incinerated the coverslips on a metal plate for 12min, to burn ...

DID YOU BOTHER TO SCROLL THROUGH ALL OF THE OFFERINGS FROM THEM??... IT IS NOT JUST "CELL CULTURE MICROSCOPES" THAT LEDS ARE USED IN... ZEISS DOES IT ALSO.. DO I HAVE TO FIND THAT AND POST A LINK ALSO??... DON'T YOU THINK THESE REPUTABLE MFGS HAVE NOT SOLVED THE COLOR PROBLEMS HERE??.. LEDS ARE THE...

I haven't had much luck with led for DF, even when one can get enough light out of them. I am using apos, and the narrow wavelength they provide, limits the information available. I'm not a fan of the heavy blue cast to the image, either. Perhaps that would be of value in reducing extraneous inform...

...how do you identify a SB or LB objective typically I look only for the 160/.75 etc and of course the threads are RMS as all I have got fit well in the turret but there is no Identifiers for that nomenclature My collection consists of 3 Nikon Phase C 10,20,40X 1 Olympus 4x non phase and rest are ...

Radazz wrote:These old illuminators usually get HOT, but I was surprised how efficiently this design handles the cooling. I could place my hand on the unit after 30 min. of operation and, while uncomfortably warm, I didn’t burn myself.

That means, that the temperature of the unit was less than about 47-50C.

Nice to have some context to go with the images. Looked up 'The Blob' (1958) on imdb, just in case it was hungry enough to come back. Good news is that it was filmed in Pennsylvania so we don't have to worry about Kurt being eaten anytime soon ;) I found some more about the Blob. The star was ---- ...

Congratulations! I do not know Kruss products, but from the photos and Wikipedia site, it appears you got yourself a quality microscope at a bargain price. Moreover, it appears to be in great cosmetic shape, and fairly clean! So I would avoid overduw cleaning. I would start by removing the slight du...

@KurtM:
An amazing direct inspection of nature in action! the soil looks like it contains much sticky clay, muddy when wet then cracked when dried up. Like the bottom of a swamp ? So was it a long-term puddle ?

@Brad: I have used ImageJ (not FiJi) a lot in the past, including programming of Macros, and did not encounter any particular problems, other than some inconvenience because of the logic of the macro commands. I ran it on a Windows 7, I7 CPU-64bit-6GB memory PC. Perhaps it depends on certain ImageJ ...

rnabholz wrote:Whatever the details, I highly recommend the inverted cure process, it improves the finished product a great deal.

Agreed. Will try to apply it.

Thanks PeteM and wporter for the info. Good to know.

...One thing you might consider is using the inverted cure process that was discussed earlier in the thread. The idea being that curing the slides with the coverslip down brings the frustules right to the bottom side of the slip. I makes a great difference in resolution and consistent focus across ...