MicrobeHunter.com Microscopy Forum

Beautiful Microscope and Microtome! I also have a weak spot for well made old stuff.

Beautiful microscope. I wouldn't use it I would display it!!!

Perhaps best not used regularly ... But definitely at least once.

Very nice. Well done!

Hi Eddie, Good news that the trinocular arrived, sorry to hear "the diopter adjustment is jammed" . May I suggest a new thread for this. With the headline something along the lines of "Jammed Diopter - Help!" Post is hiding under "Canon XTi" at the moment. Better if it were in Microscopes and optics...

Seems to be a more than a little vicarious shopping going on!

A very beautiful microscope and a most interesting article.

Hi gekko,
I can achieve Darkfield with my 6.3x, my 10x and my 16x in Phase 3 position. Easy as pie.
In theory I understand it should be possible to achieve Darkfield with my 25x and my 40x in D position.
No luck so far. Will try with oil at some stage. Do not have any oil yet. Will try to obtain some.

That is way better than a barber's razor. Well done.

Hi iBeta, Not sure how much of a problem that is. What I would suggest, seeing as you have the Digicam, is to see whether it is what you need. If it does not fulfill your expectations you can use the the pixels problem to replace it or perhaps to even get your money back. For $350 USD you may want t...

Nice images.

Do you mean like a barber's razor?
Whatever it is it certainly works well.
How do you keep it sharp - do you use a leather strop?

Post photo of it please.

Hello Sunrune, US$6000-ish I would suggest that your first microscope not be that costly. The reason for that is avoid a potential expensive white elephant. Jumping into the deep end on what might turn out to be a whim (if you do not get seriously bitten by the microscopy bug) would be a shame. Thin...

I thought at the time I had the correct annulus and objective combination.

So it was a phase objective.
And either correct annulus and not centered correctly or wrong annulus correctly centered or wrong annulus and not centered correctly.
Will play around and see what I get.

Good start. Looked up your MBU-4, there is one for sale on ebay. Make that great start. The real trouble is that my lighting is external lamp I have found that using a led flashlight with the mirror gives quite good results. I assume you have the mirror? Agree with einman - microscope photography ca...

Have a NeoFluar Pol 25x/0,60 and recently picked up a Ph2 NeoFluar 25x/0,60 Interestingly if you have a phase condenser and offset the annulus... Previous image is of my Phase condenser. What objective were you using when you achieved the Darkfield effect at 1:35 ? Nice video and Vorticella colony. ...

Getting back to the subject of the algae, take a look at these photos: 40x objective

Lots of interesting things inside that alga - have you tried stacking?
http://www.microbehunter.com/forum/phot ... -practice/

Hi The QCC,
agree they are a pain.
I think that Oliver is a little too busy to read all the posts.
It would be best to PM (private message) him to give him the heads up.
Link the offending posts to save him time.

I'll throw in this image just because I like it. 10x objective

I like it too. I have no idea about the "hairs"

Thanks The QCC and gekko. Just been rereading some old posts on my Phase condenser in the photomicrography.net forum. It would seem that I would have to oil the slide to achieve Darkfield with my 25x and 40x. Was hoping to avoid the messy stuff! Had thought/hoped it would be easier. http://i1368.pho...

Shazam! Parfocality!

Personally, I love simple solutions, even if they are (in hindsight), very simple. Well done!

Whatever - in my view the darkfield IS darkfield and the Rheinberg IS Rheinberg(the focus may be a tad out but they are still good images) I do not think anyone was criticizing Eddie's images as such. Constructive criticism if anything. They look great through the eyepiece, but I have difficulty ad...

I've not seen Abbe condensers with 0.9 apertures.

0,9 Zeiss Flip-Top

Interesting remark there, I didn't know that "student-proof" could be an euphemism for less-versatile. In this respect, a few days ago I contacted Motic asking for upgrading possibilities (mainly darkfield and phase-contrast), and it seems to be easy, in fact I saw a video about this and all you ha...

If I may butt in, and I may well be wrong, but I believe what 75RR is describing as out of focus may be areas that are overexposed resulting in loss of detail, thus giving the impression of being out of focus.

Will split the difference: 50/50

If your condenser has a numerical aperture greater then 1...

Had not considered this. Could that be the problem?
My Abbe condenser is 0,9
Not sure about my Phase condenser probably 1,3 or 1,4
My objectives:
2.5x/0,08
6.3x/0,20
10x/0,25
16x/0,35
25x/0,60
40x/0,65

Hopefully this will all be a little easier once I get the trinoc head (which was recently scanned in New York by the way!). Good news on the trinoc! Will get a lot easier (well more comfortable anyway). What you should try to achieve is parfocality with the camera tube and the binocular - that way ...

Having problems getting Darkfield with objectives above 16x i.e. 25x and 40x

If your condenser has a numerical aperture greater then 1 ...

Had not considered this. Could that be the problem?
My Abbe condenser is 0,9
Not sure about my Phase condenser probably 1,3 or 1,4
My objectives:
2.5x/0,08
6.3x/0,20
10x/0,25
16x/0,35
25x/0,60
40x/0,65

These darkfield shots are very challenging for me. They look great through the eyepiece, but I have difficulty adjusting my camera to get a good image.

Are you using a tripod? DF images look slightly out of focus.

Love the title "A Few ..." do you have a round number?
Amazing collection of microscopes in what looks and am sure individually are, in pristine condition.
I take it they are not shelf queens, so you must drive them round the block - so to speak - from time to time?

Hi bartmart, a word of caution. Reading through the specs of the BA210 LED Trinocular I noticed this ominous phrase:  Student proof features ... This effectively means that some parts are fixed or at the very least can not easily be removed. This could make upgrades difficult or even impossible. Yo...

Interesting algae. What magnification was the objective that you used with Darkfield?
If it is a 25x or 40x do tell us how you managed it. I can't get past 16x.