MicrobeHunter.com Microscopy Forum

I think touplite (lower case) is the .png icon. I think I'll just need ./ToupLite (or whatever the file is; can't see it now). We'll see. Lots of playing around = "no harm, no foul." I'll get this!

Yes, the 32 bit install was my error. I will purge and install the 64 bit version. Yes, it's now confirmed that I must ./ from usr/local. I think that that read only output gives me the name of the file that I must execute. I live in the Dominican Republic and the electricity has been off since yest...

OP: So in brief: successful install; run fail.

@dtsh I am running Ubuntu 20.04.2 LTS Here's my complete output: From touptek.com I downloaded ToupTekToupLite.x86.tar.bz2 Terminal: tar -xvjf ditto Terminal: sudo chmod +x ToupTekToupLite.x86.sh Terminal: ./ToupTekToupLite.x86.sh Terminal: install ToupLite ok Then I get this output (a doc. generate...

For me success and failure . . . I must vet and successfully run this program before I buy the ~$600.00 camera (5.1 megapixel Microscope Camera with Basic Software DCM5.3). Hardware is only as good as its software. After I downloaded the program I extracted the zip file and got ToupTekToupLite.x64.s...

I’m thinking about plunging feet first into photomicrography. I first began a discussion with tech support at microscopeworld.com and resolved that I would opt for a dedicated camera via c-mount as I did not want to jury-rig a DSLR via eyetube with a t-mount, as my phototube/c-mount precludes a DSLR...

My DF slider has two click-points that “lock” it into the condenser, one for BF and one for DF. When I lock the slider into the DF position, I have a luminescent specimen and a bold black background. The problem is that in the center of my FOV I have a large roundish blurry and brownish artifact tha...

Seems like that could describe a lot of organisms I wasn't thinking. I had at my disposal how to know the protozoa , Second Edition, 1979, Jahn, Bovee, Jahn. It is published by William C. Brown and it is part of the Pictured Key Nature Series. When I first looked at the key, namely, the entire book...

@MichaelG Remember the link you gave for the Zeiss Innovation issue on Abbe and his experiments? It's a good thing I browsed through that because I found out that my assumptions about how I would employ a diffraction grating were completely wrong. If you recall the beginning of Evennett's lecture on...

MicroBob wrote: ↑

Mon Jun 21, 2021 3:21 pm

The link didn't work. Is it this you meant?
[urlhttp://bigwww.epfl.ch/demo/edf/
url]

It would be interesting to compare it to Picolay

I checked it. Just try imagej.net and navigate from there.

Probably most of you know this site, but for those who don't: imagej.net/software/fiji for OS's Windows, MacOS, Linux . . .

I found this in the Online Resources section of of Matsumoto and Roullard's An Introduction to Digital Photomicrography, The Crowood Press, 2017

From tree bark infusion: --transparent under BF; viewed hundreds on three drops on a depression slide --ovoid shaped with large asymmetrically located nucleus --no flagella; motility by twisting/spinning motion; very fast (10x) --filled with discrete particles that I cannot resolve Not set up for ph...

The whole ‘Abbe Diffraction Apparatus’ is illustrated here: https://www.nms.ac.uk/explore-our-collections/collection-search-results/abbe-diffraction-apparatus/647239 MichaelG. . Edit: __ See p18 et seq of this : https://www.zeiss.co.uk/content/dam/Corporate/pressandmedia/downloads/Carl_Zeiss_Innova...

Check out the calibration and stage micrometers section of Electron Microscopy Sciences - the micrometer standards section looks like it contains the same type of slides used in the video. https://www.emsdiasum.com/microscopy/products/magnifier/stage.aspx#stage I think that if any of these items we...

After Peter Evennett's lectures on conjugate planes and some readings on optics in Matsumoto and Roullard's An Introduction to Digital Photomicography , I want to see various diffraction orders and their relationship to NA and resolution for myself. But I need the same grating, a slide, one of opaqu...

@Dubious Yes, so opacity in BF is an artifact. However, BF gives me superior resolution of internal versus peripheral features. It will be interesting to compare colors of specimens BF vs DF. Some artifacts (a bubble) can be ignored; others, although lacking in one area, provide useful information i...

Surprising Color Change Observation

When I look at one of my cockatiel's yellow feathers in brightfield the color is black and various shades thereof and I have excellent resolution. When I change same to DF the true color (yellow) appears! How can this be??

How to Remove an Artifact I'm looking at a specimen with proper DF. At a certain brightness, when I turn up the illumination, I see my DF stop in the center of my specimen! Turning down the light causes it to vanish but then I lose some resolution because the image is too dark . . . Does the artifac...

I have a solution for centering the stop which I found at https://moticeurope.blogspot.com/2016/08/introduction-to-darkfield-illumination.html Not having a centering telescope, I place a rectangle of graph paper on the stage. With illumination turned up under DF, I can confirm that the stop is cente...

Never having used a darkfield slider, I first discovered that after removing the plastic slider stand-in, that I could not angle in the DF slider without force even though both widths were identical. A Net search turned up much on DF illumination but almost nothing on hardware implementation. The on...

@BramHuntingNematodes @Hobbyst46 Thanks for sharing your experience and knowledge with color filters. It turns out that the filter I could not identify is a blue filter. It doesn't appear blue at a short distance but when I hold it up to the light it is indeed blue. And my original order confirms sa...

Many microscopes, mine, for example, come with a set of color modulating filters--and no instructions. When I research their use, the information I find is rather thin. It appears that the blue or yellow filters may compensate (balance) light from a tungsten or halogen bulb. Now that many of us use ...

@MicroBob

I think I'll start looking in my eye tube while adjusting various parameters and see if I can infer what I am looking at.

@75RR

Evennett is great. He explains simply, slowly, and confidently. I have downloaded the video. Thanks.

I have found that the packaging for Electron Microscopy Sciences micro cover glasses facilitates ease of taking single covers. Here is EMS's webpage for them: https://www.emsdiasum.com/microscopy/products/preparation/cover.aspx#63750 I don't know what it is that was done differently with the packag...

OP Add-on: I've been reading about culture media for protozoa at microscope-microscope.org There is one item in these directions that I don't understand: [The "hay infusion" is perhaps the most well known culturing technique. Boil one liter of pond, spring or rain water. As the water comes to a boil...

First of all, press upwards, lightly, with the tip of your finger, on the end (bottom point when installed on the nosepiece) of the 100x objective. If it moves up easily, it is spring loaded (99.99999% it is). I checked, placing a lens tissue over my finger. I was surprised to see that what retract...

Our most important tools are the slide and coverglass; the pipette or dropper. A microscope slide dispenser is very useful. A member here recommended emsdiasum.com or Electron Microscopy Sciences. This is an advanced site for LM and EM but favoring EM. Buy from them and they will send you a pack of ...

linuxusr wrote: ↑

Sun May 30, 2021 2:19 pm

@MicroBob

Could you please give a brief description of how I use a Bertrand lens as well as how I buy one? Also, to view surface placement of the oil, why would I want to view the back lens and not the front lens?

Repeat for MicroBob or anyone else!

Most of the high NA condensers are intnded fo use with oil, I don't think one can get the high NA without it. Do you know what model yours is? It's NA? My limited understanding of it says that it is typical to oil slide to condenser and slide to objective. I'm sure there have been setups that devia...