MicrobeHunter.com Microscopy Forum

Hi Oliver...my sense is we all understood the need for protection from 'the bot's'...and fun is a conciet of my wetware. I totally like all the tools this platform permits for forum microscopy. charlie guevara' BTW ,Karl, I was trying to 'up your posting number' with the fanciful banter...keep at it...

Your color suggests springtime is coming, 75RR. Beautiful images you have. "And spring herself, when she woke at dawn, would scarely notice, mankind has gone." /There will come a soft rain, Sarah Teasedale (?sp?)

Thanks, John B.. charlie guevara

Hi, 'depresion well slides' or 'concave depression slides' are often handy for water fleas, hydras, a tuft of algae which has complex structures, water mites, flat worms...and the eggs of so many wee creatures...they work well (pun) either single well, or two wells per slide. Of course many will pla...

Roger that, loud and clear!

A YouTube plaftform...wonderful for me! charlie guevara

Beautiful results,John B. The orientation achieved by your agar pour is sweet! The tissues to my eyes are not distracted by the agar inclusions..it's sort a background effect. But how will stains affect this background agar? Thanks for keeping at it, we appreciate and learn from your microtechnique....

Visit your own fish tank...or a friends fish tank! Cheap waxed paper 6oz cups...one for the house plant catch dish fluid, one for the fish tank filter water sample, one for a gentle scraping of sludge/biofilm from side of an aquarium. Mark the cups:A,B,C to recall which is which. For each add water ...

Again thanks for this brief day in the life , for curreent live cell microscopy, kinase. charlie guevara

Fascinating links, 75RR, thank you. I love Pau's images of those flagelates. I have a wrongheaded approach to my wetmount slides...I use the large rectangular (22X44mm) slips...but these (as zzffnn has noted for me) are the most variable in thickness spec! I admire anyone who has the courage to use ...

Thank you, Glen. So there is at least one person who (like me) senses the hyphen engraved on high mag/high NA/oil objectives does not mean: 'you use with a slip, you use without a slip, and thickness of slip not an issue'.

I wonder why it came to be? charlie guevara...all the best .

How can this non-correction collar high NA/ high mag objective seemingly...'do it all'...no worry of spec of cover slip...and no need to use cover slip if you don't need to ( as in a blood smear)....how does this plan apo / 1.4 NA objective 'do it all'? I do not think it does it all! The Hyphen sym...

Hi, gekko...this thread ( which I think I hijacked on Glen...Glen...I owe you...seriously.)....this thread drew me to ask: 'What am I missing,,,how can a high NA (1.4 NA), high magnification (63X), highly corrected (Planapo), oil-immersion objective...work with no coverslips, work with cover slips.....

Do androids dream of electric sheep?

Beautiful equipment,kinase...thanks for the tour. Did your operational training occur 'on the job'? Or did you have formal traing before being put to work with these systems? Are the 'company reps' a big part of your training? Live cell microscopy in the land of research..wonderful to have had your ...

Thanks for this advice...I forgot about my 'send folder'. Anyway, Fan...at 38 of allowed 100 PM's..I still am not allowed to have the new PM. charlie guevara

Beautiful, vasselle! Based on how plump this diatom is, based on it's two chromatophores, based on the rounded poles, and all the rich detail in the topmost pole...I'd say this is Genus: Gyrosigma.

The striations in this valve view are terrific! charlie guevara

Wow..I did,nt know of the rule of posts! I say Karl is real! In an odd way..the rule of posts..(which I did not know about)..I guess the rule of posts is now out of the bag...so 'empty posting' will be selected for..to permit use of the PM feature. It's good Karl mentioned his speed bump prior to en...

Beautiful rotifer, BillP...with image captures like yours...I can open some of my texts and literally enjoy learning about this meiofauna tribe! Thank you. Your music score kept me waiting for Stevie Nick's smokey and beckoning voice in their smash hit (Fleetwood Mac, "Players")...ah to be young and...

Hi, all, I just err put my seat to the computer room chair...I'm alerted I have a 'new private message'...I go to this great forum/log in..."mesage is pended due space in the folder...it 'may be available' (what's this!!??) if space opens up for it. 1) at the time I had: 50 of 100 PM's in my PM inbo...

.you can not have as highly corrected (planapo), as high mag (63X), as high NA (1.4) ...oil immersion objective which functions optimally despite the variations in cover slip thickness...or 'and it also functions optimally' with no coverslip...these optics do not exist! But they do exist (not commo...

Hi all...and thanks for this thread,75RR. What am I missing?!! How can this non-correction collar high NA/ high mag objective seemingly...'do it all'...no worry of spec of cover slip...and no need to use cover slip if you don't need to ( as in a blood smear)....how does this plan apo / 1.4 NA object...

Charlie, here is a link to information from Zeiss. Please note under Tube Length/Cover Glass Thickness that it says: without cover glass: 0 insensitive: - http://zeiss-campus.magnet.fsu.edu/tutorials/objectivecolorcoding/ Instead of 0 for "no cover glass", some manufacturers use NCG or NC . So 160/...

We all are eager, John B., yes, yes! So your doggie moved the infiltration schedule along!

I fondly recall Vincent Price (House of Wax, 1953?) had an eries wax process of specimens!

thanks for the update...wonderful learning experience you share. Charlie guevara

... your objective etched spec: " 160/-" implies: 'do not use with a cover slip!!?? What am I missing. Charlie guevara I believe it means that the thickness of the cover glass is not critical, and the objective may be used without one. I believe an objective that should not be used with a cover gla...

Once I'm home...I can peek at my B&L metallurgical objectives...but they might actually print out the entire directive:"use no coverslip.". Charlie guevara

It sounds like blow by blow microscopy 'ice fishing', Dennis! I promise to myself ( so no forum folk hold me to it) to try Olivers nail polish protocol! Charlie guevara

You said 'it worked for you'...but I just can't see snow flakes sinking into the vicous nail polish goo. I trying it though. Charlie guevara

Wow, Oliver...your nail polish method sounds great...I'm giving it a try! thanks for the tip! Charlie Guevara...I think i't in 20's F now .

Thanks for the terrific chart and showcase of variety of their line of objectives...I love to window shop!

But 75RR..the chart is for a 63X objective with spec: "160/ 0.17" your objective etched spec: " 160/-" implies: 'do not use with a cover slip!!?? What am I missing. Charlie guevara