MicrobeHunter.com Microscopy Forum

Is ten microns really the thinnest sections you can get with a hand microtome?

Throughout browsing numerous texts on micro-technique I can't help but see an abundance of use of glacial acetic acid. My country doesn't have a easily accessible source of this reagent. My question is can I the much weaker 5% or vinegar as substitute? Also are there any acids, easily accessible tha...

For some reason I cannot see certain user's post. Such users like Yvan or other posts of mrsonchus. It's really weird.

I found this website the manufactures various optical components including microscope objectives. They seem to sell planapochromats for DIN and infinity systems. What's your thoughts?

Here's the site: focusinc.ru

Well i've found that my microscope accepts 37mm condenser mounts so the condenser can definitely be situated, my question are there other things now that'll prevent usage of the condenser? Like are condensers similar to infinity optics in that they are not compatible with other microscopes despite h...

Hello so I have a question, my default condenser has an external diameter of approximately 36.92 to 36.7mm, the Lomo aplanatic condenser has a mounting diameter of 37mm. Will the condenser fit with the rack and pinion holding my current condenser? My microscope is the M82es

Thanks zzffnn but right now I am looking for options but once I have enough money I'll contact you.

Other than eBay, are there any websites that sell Lomo objectives?

The company, Cnscope which make these set of objectives:http://www.ebay.ca/itm/New-DIN-Plan-Achromatic-Microscope-Objective-Lens-Sets-4X-10X-40X-100X-/221434856994?hash=item338e8bc622:g:O5gAAMXQ0pNRpfjW said that they were related to companies such as Olympus, Leica. Here's their website:http://cnsc...

Does anybody have a resource on Lomo apochromats?

Well the microscope I am using is an Omax M82ES with their stock achromats. Will these plans improve image quality compared to the stock achromats.

Also, I would want to know if the objectives are a good investment to upgrade my optics.

Well I have a parfocal adapter for short barrel DIN objectives, so that issue can be resolved. Does Lomo really require compensating eyepieces for their objectives?

Which would a better investment in terms of quality:
A set of Plan Objectives such as this one: http://www.ebay.ca/itm/New-DIN-Plan-Ach ... XQ0pNRpfjW
Or finding various Lomo Achromats

Does anybody know how to tighten the focusing mechanism of an Abbe condenser?

Yvan if possible could you ship a portion of your acid to Canada inexpensively?

Yeah and shipping prices and restrictions are a pain.

I've search far and wide and coudn't find a source of this invaluable fixative. Does anybody know where to find Glacial acetic acid that's relatively cheap in Canada?

Yvan, you're making me worry with your "OOOOON the other hand".

A while back I asked whether isopropyl alcohol can be used a fixative. The kind and humbled mrsonchus confirmed this. I also referred to Stainfiles but most sources said the isopropyl alcohol is used as a dehydrant. Sources also said that isopropyl is a poor fat solvent. Regardless though, for the g...

Thanks Rnabholz but unfortunately the slides you mentioned don't ship to Canada

Other than Ted Pella, does anybody know where to buy microscope slides in bulk? Preferably in the 500-1000 amount very cheaply.

Yvan for the tissue preparation, you said that isopropyl alcohol should be used instead of ethyl alcohol for tissue processing. Does this apply to both the fixating and dehydrating processes? If so, is there a distinction between fixating with ethyl or isopropyl alcohol.

I have a question regarding the prices for the economic slides. Do I theoretically pay 9.80 for let's say 10 packages of the blank slides or do I pay 9.80 for every pack?
Link regarding the product in question: http://www.tedpella.com/histo_html/slides.htm#260440

Yeah mrsonchus, I want to know if plant sections require clearing agents for permanent preparations.

For plant sections, is clearing really necessary? Sections of plant can be scarce of cell contents but I want to know if the clearing process is required for high quality preparation.

What are lubricants and degreasers to make mechanical parts of a microscope run more smoothly.

Let's discuss various ways to assess objective clarity. Resolution and "planarity" are our main focuses for this discussion. I would use Cheek Cells to assess resolution, while not as convenient or as discerning ad diatom species, it sure is an economical alternative. What are some other ways to ass...

Actually the reason why I needed the extender is because I have to raise my stage so high up that it touches the spring loaded high mags objectives. This lowers the objectives so that the others would be fine.

Another thing is whether a adapter is needed for Zeiss Jena DIN's