MicrobeHunter.com Microscopy Forum

Similar dynamics occur in other industries; I was employed by a company that moved manufacturing from the U.S. to Korea, then China. Engineers were in the habit of walking from their offices and labs to better explain or correct their incomplete or wrong documentation on the manufacturing floor. A K...

expensive manufacturers seems to put more weight in the light spectrum. Low cost and efficient white LEDs indeed have problematic color rendition, with some being more nearly blue + yellow than red, green and blue. Sourcing more expensive LEDs with better color rendering helps mitigate chromatic ab...

This is pretty powerful, but I had some strange halo on my microscope. I think LED is the culprit, but maybe not. I bought another microscope with complete lighting meanwhile so stopped searching. Microscope collector lens for halogen bulb is designed for relatively small square filament; larger ar...

woyjwjl wrote: ↑

Fri Apr 21, 2023 1:26 am

Which setting do you prefer?

None appear to have appreciably more detail,
but converted to grayscale, the left has more contrast that the middle.

The Olympus BX 53 appears to use infinity objectives, with no corrections in oculars. If so, then an afocal setup over the photo port should work with no relay lens. For Canon APS-C, an EF-S 55-250mm zoom should work. Those are available used for less than US$100. Once a focal length which nicely fi...

Rorschach wrote: ↑

Fri Apr 07, 2023 5:55 am

it has the correct 10x WPK eyepieces

I was thinking more about photo correction.

Literally a fantastic scope.
Here's hoping correcting trinocular optics are included.

The finite Nikon heads (___ phot) I've had measure around 48.5 to 49mm+ at the largest dovetail diameter - even the old black stands. I'm pretty sure B's cleverly-modified Tc head is the same. Actually, Tc dovetail diameter is indeed about 51mm, but it seemingly lacks infinity space around prisms, ...

Nikon TC head will fit to E200? TC head has a dove-tail diameter of about 51mm According to Mike Tuell, Eclipse shares Labophot/Optiphot head dovetail diameter (49mm?) https://lavinia.as.arizona.edu/~mtuell/scopes/Eclipse.php Removing the bottom lens from a (more common) Labophot/Optiphot T or UW h...

https://icy.bioimageanalysis.org/ I suspect this will be a very steep learning-curve Thanks for the pointer. While attractive, Icy's home page failed to quickly convey (to me) why one would choose it over e.g. Fiji or ImageJ, to which it is related, for which this page helps a bit: https://www.bif....

45mm Diameter Microscope Optical Diffuser Frosted Glass
https://www.ebay.com/itm/275090743723

JCTyler wrote: ↑

Wed Feb 15, 2023 9:59 pm

So what would putting an adapter tube shorter that 45mm do?

Much depends on objective optical design specifics.
https://blekenbleu.github.io/microscope/basics/#TL

Depending on your applications, micrometer slides with and without coverslips are wanted.

I will take a look at that. It is has both constant voltage and current 10-turn pots; one comfortable with soldering could replace a 10-turn pot with a remote single turn control. I use one to supply a substantial supercapacitor bank, which takes several minutes to charge at about 4.5 amps, during ...

JCTyler wrote: ↑

Wed Feb 15, 2023 2:40 am

Its a finite distance objective lens. Its a Nikon BD plan

Nikon sold adapters for that purpose, e.g. to use M Plan objectives in a BD turret.
Those objectives are 210 tube length; a few mm has about 1/4 less impact than for 160 tube length objectives.

Scarodactyl wrote: ↑

Mon Feb 13, 2023 10:19 pm

If they ever make a 3amp buckblock I'll be first in line.

https://www.amazon.com/dp/B07J9RQ8B6/

I replaced a missing Optiphot illumination diffuser with a very generic glass round of matching diameter: https://www.amazon.com/dp/B0041V9IX4 Note that it is described as "frosted", rather than "ground glass" or "diffuser", offering more search options. IMO, illumination diffusion is a relatively u...

sreynolds wrote: ↑

Thu Feb 02, 2023 3:39 am

filter holder to the .90 Achr flip-out condenser on my Labophot

See https://www.microbehunter.com/microscop ... 14&t=10263

can it be used for transmitted light DIC? Some were so equipped; many were EPI only. Search is your friend; I use Google, adding quoted key works, e.g.: site:www.microbehunter.com/microscopy-forum/ "polyvar" "dic" https://www.google.com/search?q=site%3Awww.microbehunter.com%2Fmicroscopy-forum%2F+%2...

You can get a 38mm od to m42 adapter and an m42 to canon ef-m adapter for even better results. RAF Camera makes high quality adapters and seems the only source for some microscope dovetails adapters. Today, I received less expensive M42-EOSM adapter from AliExpress that fits well. M42-EF.M.jpg

I'll try with such a G4 COB 3W LED with a 360° lighting in place of the previous halogen lamp (20W)! But I wonder if a classical dimmer for such low voltage Led could be efficient, as it also seems there is electronic items inside this LED.... My experience has been that, if LEDs are not advertised...

My Labophot has Variant 2, the most commonly seen tube arrangement. How do I convert to the setup with the short tube? I have never handled a Variant 2. Based on eBay images, it seemingly a) like variant 1, has no optic at the dovetail flange: https://i.ebayimg.com/images/g/YAAAAOSwxrNjkNEA/s-l1600...

Would this adapter allow me to connect my Olympus OM1 to the T mount? https://www.amazon.com/gp/product/B00DQ0MHM0/ref=ppx_yo_dt_b_asin_title_o07_s00 Sorry, no. That adapter is for using Olympus OM-mount lenses on Canon EOS-M bodies. Hint: OM bodies are DSLR, so space between flange and sensor for ...

"...focusing on cellular organization...at the level of major cellular structures that represent distinct organelles and functional machines" https://www.nature.com/articles/s41586-022-05563-7 Allen Institute Cell Catalog https://www.allencell.org/cell-catalog.html QuickViews: https://www.allencell....

imkap wrote: ↑

Mon Jan 02, 2023 8:16 pm

It has an iris and some options, even an LCD screen

Those smaller tips should work better near microscope objectives than do more typical 13mm tips...

So you think we might be ok without these for a start? Yes, you may discover that additional illumination concentration is unwanted. I mostly use my cold light with an AO Cycloptic, at which magnifications and illumination field diameters such lenses are inapplicable. For higher magnifications, e.g...

The only thing missing are the focusing lens, which should go to tips of the goosenecks. Maybe I could source these somewhere I have not needed those, but: https://www.edmundoptics.com/f/fiber-lite-focusing-lenses/11349/ Also, FWIW, extensive use of halogen bulbs well below their rated power accele...

The Swift 380T is a finite (160 tube length) microscope; except for Nikon since 1980 or so, most finite microscopes depend on eyepieces to help correct objective aberrations. I also own and recommend those WF10X/22 high eyepoint eyepieces, but they provide no corrections and thus work best for moder...

Not expecting to frequently use backlit illumination, and rejecting as excessive previous suggestions, e.g.: Stereo Biological Microscope Bottom LED Backlight Light Source https://i.ebayimg.com/images/g/w40AAOSwNYBi-vnh/s-l1600.jpg .. a smartphone screen displaying bright white should suffice, e.g.:...

US$60 for three, $25 for one, 18" W x 20" H, maximum circumference of 32":

https://benzmicroscope.com/products/mic ... yl-bzdc-xl