MicrobeHunter.com Microscopy Forum

Since you are posting images from a camera mounted on a trinocular, is the same level and spread of ca, evident through the eyepieces?

Those micrometer images . Are they a center crop or if not can you provide a dead center micrometer image?

What microscope is this and how are the pictures being taken? Cell phone through an eyepiece?

So, I think what is happening there is that the extension tube is most likely as I suggested for a 4X5 Polaroid back, so your little postage stamp sized sensor is only collecting a fragment of the image. If you went to an APS-C mirrorless back like the Sony, which has a sensor size almost the same d...

Unfortunately, you cannot make a silk purse out of a sow's ear or an apple pie out of shit from an apple eating pig. Chromatic aberration is the result of refraction which is the most obvious to us of the several aberrations and distortions that occur when light moves across a refractive border. Fil...

To most microscopists, this means centering the condenser under the objective. What if the condenser itself isn't aligned? A condenser is an optical device that focuses light along an axis but also usually includes a mechanical device that reduces the intensity of the light by vignetting the periphe...

The Polylite, Polyvar and Polycon bridged the gap across the AO/Reichert/Leica brand transformation. You can find AO Polycons and Leica Polylites, which were white : maybe even Leica Polyvars. The models also bridged the gap across several image capture formats. Polaroid was common for them and a fr...

The illumination is "Built-in transmitted Koehler illumination6V 30W halogen bulb (pre-centered) 6V30WHAL (PHILIPS 5761)" according to the BX41 manual. So for something with Kohler illumination, infinity objectives, the ability to swap out for a darkfield condenser down the road, and a camera tube ...

You are getting pretty flat imaging for plain achro objectives. Your pictures are quite good for a simple system.

You are paying a lot for that Olympus if it is a binocular 4 plan N objective microscope. What is the illumination? Buying a 5 planachro objective BF scope that would duplicate the performance of that Olympus is a less than a 1500.00 project : less than 1000.00, really. Use the extra money to buy so...

Does the Polylite have 23mm eyepieces or maybe I am thinking the Polycon. edit. I can see now in the picture you posted that the eyepiece is the same as for the Polyvar. The Polycon had eyepieces that were corrected like those on the Microstar/ Diastar. The trinocular setup on those may bypass the v...

It sounds to me like you are paying about $1,000.00 more for what you are getting, in both cases. You didn't detail which Amscope you are buying but as a general rule, if you are not getting a trinocular head, oil immersion DF condenser with an iris equipped 100X objective , at least some objectives...

Your filter cutting looks o.k. to me but in these types of lighting adaptions geometry and materials selection are as important as precision. I am wondering if you have tried varying the slit width much and used rotated assymetry ? It seems that in some ways you are making a modified diy version of...

The 506289 objective was never a derivative of the Reichert Plan Fluor objective family. They came from two different factories, had different reference focal lengths and did not share optical design . The PL S Apo 10x objective has a 11mm working distance as compared to other Apo 10x. This is why ...

Hi, all There is a Leica HCX PL S-APO 10x0.30 on the market, ID 506289. However I tried to search in Leica website and google, there is no such record, only a paper. https://bio-protocol.org/exchange/minidetail?id=10271842&type=30 I guess this S-APO is not really the APO, maybe specially designed f...

Questions like yours are hard to answer unless someone has had that particular mechanism apart, because despite the similarity of function between dropdown coaxial stage controls, there are various ways to design them. i know of one design for instsnce that pressfits a gear to the end of a shaft on ...

In former times, there were little adjusting screws on each objective port on some stands. These were over time deleted in favour of more precision production methods. Metallurgicsl objectives have always or at least for 100+ years had a method of centering individually mounted objectives using eith...

Oddly, those are listed as used. That might suggest that they are some that were returned. The flocking is required at the bottom ; 1/2" up will do it. Another option and perhaps a better one is to find or make a thin aluminum washer with just a little smaller i.d. and solder it on the bottom. That ...

Your filter cutting looks o.k. to me but in these types of lighting adaptions geometry and materials selection are as important as precision. I am wondering if you have tried varying the slit width much and used rotated assymetry ? It seems that in some ways you are making a modified diy version of ...

That is definitely true of G4 sockets. in fact one of the easiest ways of focusing a G4 bulb is just to position the filament by bending the little pins this way or that. In this case though, the illuminator is a slide in from the back up to an adjustable stop which can be set to allow the end of th...

There are a number of reasons for a lens or 2 to be in the photo tube, depending on the original desgn of the microscope. When the tube is mfg. specifically to receive a certain camera or sensor size, the lens could be a framing lens of a certain magnification , so that the projection matches the se...

I have found DF to be surprisingly 3 D, even at high apertures. Right now I am playing with pol + Göerz slit condenser which has DF caps up to 1.2 N.A
Fusion of DF and pol

Your images and techniques are very good.

The most common 6v. 20 watt halogen bulb used on microscopes is the ESB. It is about 1/2 an inch long, with a 2 pin socket ( G 4), and a little dollop of hardened glass on the end that makes them look like miniature soft ice cream cones. They are installed sideways. There is another version of a 6v....

Maybe a Baijiu inspired version. I can almost hear the after work discussions weighing a 20 vs. a 25. I wonder if somewhere out there is a 45X or maybe even a 50..

I did an objective search at Silo for 25X , which are almost all European. B & L made a fantastic 25X .65 Flat Field apochromat for the Balplan but outside of that 25s are scarce and a 25X on a Chinese scope is quite odd but there may be more 25x formatted outside the .17 or .18 cover types I am mos...

The knockoff AO photo tubes beiing sold on ebay have the same problem but it doesn't look exactly like that. I thought about it in your case but decided not to bring it up in order not to lead you away from the real cause, which did seem to be a focal point problem. Glad you found that solution. In ...

If I recall Olympus , in their official history, brings up the M & Katera connection and the first mass produced microscope in Japan but in actual fact Olympus was a thermometer company by the name of Kogaku Kikai Sensakusho when that model was made by M & Katera, the direct corporate ancestor of Ch...

I see one out there. Message sent with a link.

Another great contribution thanks. Striving for perfection in production is the biggest waste of time and money resulting in bankruptcy or buy out. There is an agreed standard, sometimes that is exceeded by chance. Below that is usually left for the phase B tester to dispute and resolve. The intend...

apochronaut, Thanks for this very informative post. If I understand your last paragraph, you deduced from position of the image where the shoulder was contacting the nosepiece, and filed that point of the shoulder down about 0.0035 inches (enough to move the contact point about 45 degrees) to move ...