MicrobeHunter.com Microscopy Forum

But the biggest disadvantage of the SB Olympus objectives, IMHO, is the too short working distance . This feature has been mentioned on the Olympus brochure. Indeed, the front lens of even the 10X0.32 planapo SB objective is very close to the coverslip. That of the 20X0.65 planapo is dangerously cl...

I don't use the 4x much at all. 10x is fine for scanning the slide, imho. And I love having a mid-magnification oil objective.

Thus, with five spots, I would go: 10x, 20x, 40x Dry, 40x oil, 60x/100x oil.

And for the one phase objective, I think you would be happiest with a 20x.

Scarodactyl wrote: ↑

Wed May 24, 2023 11:53 pm

I remember the crazy field number. Did they ever make any accessory that could actually take advantage of it?

Yep, the BiSW 7x eyepieces have a FN of 29mm! The 10x are 26.5mm.

I cannot seem to find the “Olympus High Quality Optics catalogue" pdf for the SB objectives at the link you provided. Also I am trying to figure out the SB phase objectives…especially if they work the LB phase condenser..there are indications that they do not. The lack of readily available Olympus ...

If you go to: http://www.alanwood.net/olympus/downloads.html Then the "Olympus High Quality Optics catalogue" pdf is a comprehensive overview of the SB optics, and the "Olympus LB Objectives" and "Olympus LB Objective Series for Biological Use" pdfs are the same for the LB ones. I own all of the Oly...

Those scratches on the phase annuli might not be a big problem, as long as they let thought a decent ring of light. That said, I never use phase since getting DIC, so I wouldn't worry about them anyway.

These are 160mm, right? Do you know the eyepiece pickup point? This data is missing from my go-to reference, Die Mikrofibel. (I'm just curious, not looking to expand my collection...)

Yeah I'm sorry for that, I realize now I should have said that it's for centering an epi illumination light source, as it's in no way specific to fluorescence.

The strange black one is for centering a fluorescence light source.

Direct projection to full frame (ideal) or aps-c would be my preferred method. A friend recently bought a BX53 and set it up for direct projection with a Nikon Z7 full frame camera using the method described in the link below (U-TV1x-2 -> U-TMAD -> T2-Z adapter -> Z7). The results are awesome. For f...

But... It's not an approximation of the Kohler principle. The principle of Köhler is about focal points and image planes. An image of the light source should be in sharp focus at the objective back focal plane. Just look down an eyepiece tube with an eyepiece removed (or use a phase telescope) and h...

Not sure if their page is up to date, but dealcorner lists a Splan 40x for $90:
https://www.dealcorner.com/Olympus-Objectives

Are these readily available in China, woyjwjl? What's the going rate? Seeing the eyepiece is standard 23.4mm, I'm definitely tempted by this!

They are just standard parfocalizing shims. I ended up with a bunch that came from a dealer of various thicknesses. I put a flat file that is wider than 20mm on them and start a bevel on them, finishing on a sandpaper pad. Firstly, I rotate the objectives around the nosepiece to find the position w...

Objectives and nosepieces with crappy machining often need to be centered using thin bevelled shims against the objective shoulder, so the objective can be orbitally adjusted. I took an over 120 micron variance out of a 60X Chinese planachro that way. Never heard of that approach, thanks for the ti...

Okay so i think im getting it. The 160mm and 170mm are both the same phisical size and i will not have to make any ajustment on the stage when changing between these two objectives. The only change is were the image they produce is being delivered, in the eyepiece-hole of the head. Is that it? Am i...

As long as you use 170mm eyepieces on your 170mm microscope, both 160mm and 170mm objectives will work ok, as the location of the image only differs by 2mm, as seen here: https://www.microbehunter.com/microscopy-forum/viewtopic.php?p=77060#p77060 I really like the 170mm Pl Fl objectives I've tried, ...

Sure, the Nikon CFN plans would probably work just fine. But they are Plan Achromats. Good achromats, but probably not a huge difference to the PZO achromats the PZO scope comes with. Personally, I wouldn't pay very much for such a modest improvment (when you can often pay less for better fluorite a...

I saw you linked a Nikon 160mm planapo. Since Nikon is kind of an outlier in terms of corrections in the finite era, I would stay away from them in a mix&match system. If you look at this graph: https://www.mikroskopie-forum.de/pictures004/166258_41519911.jpg From: https://www.mikroskopie-forum.de/i...

To find out what mismatcehd combinatiosn are compatible, you either need to find ask someone who has tried a specific combination, or you need to take a risk and try it yourself. There really is no other way around it. If you want a system that you know will work 100%, you need to buy a set that was...

Yes for sure, I have used Wild 10x eyepieces as collimating optics for light sources before, and they seem very good for the job.

Search Google for:
"Zeiss Standard RA" site:microbehunter.com

There was a pretty fascinating thread where the founder of InFocus joined this forum: https://www.microbehunter.com/microscopy-forum/viewtopic.php?f=4&t=11305 The "exaggerated, self-aggrandizing tone" definitely reads more like a advertisement for Sea-Monkeys at the back of a 1970s comic book than a...

Good buy, the Microphot is a magnificent scope, and seem to quite often have been sold with DIC. As you probably know, you will need 160mm Nikon CF objectives. Most of them are covered in this catalogue: https://krebsmicro.com/Nikon_CF.pdf Many of the objectives shown in the catalogue are of the upd...

I think George, that your best bet is to go for those W.F.10X/18 glasses on eyepieces( with the little spectacle image)from your second link. Those will be correcting eyepieces. Are you sure about this? I think I have an identical pair lying around somewhere, I have no recollection at all that they...

The Swift 380T is a finite (160 tube length) microscope; except for Nikon since 1980 or so, most finite microscopes depend on eyepieces to help correct objective aberrations. I also own and recommend those WF10X/22 high eyepoint eyepieces, but they provide no corrections and thus work best for mode...

22mm is the width of the image circle, not the tube width. But these are for 30mm tubes, not 23.4mm. So they will only fit on superwide heads, or many stereo microscopes. Thanks for the answer, I found a different eyepiece with a 23.2mm diameter (but I can't find a 23.4mm). Would this work? https:/...

https://www.microscopyu.com/techniques/fluorescence/nikon-fluorescence-filter-sets/ultraviolet-excitation-filter-sets/ultraviolet-excitation-uv-2b-longpass-emission Nikon's UV-2B cube is very popular, and likely the most useful one for an amateur wishing to photograph autofluorescence. It has a slig...

22mm is the width of the image circle, not the tube width.

But these are for 30mm tubes, not 23.4mm. So they will only fit on superwide heads, or many stereo microscopes.

It's not the shutter speed that freezes motion - it's the flash. The duration of the flash is extremely short when used at 1/8 power or less. What you need to do is reduce the power of the continuous illumination. Then set an exposure time that's long enough to trigger the flash (say 1/160s). The go...