Search found 431 matches

by viktor j nilsson
Tue Mar 16, 2021 3:54 pm
Forum: Pictures and Videos
Topic: My first live samples under the microscope
Replies: 5
Views: 422

Re: My first live samples under the microscope

Looks very promising!

Minor correction: if it's the CFN Plan 40x, it has a NA of 0.70, not 0.65.
by viktor j nilsson
Tue Mar 16, 2021 11:57 am
Forum: Microscopes and optics
Topic: Mosquito wing for objective testing
Replies: 18
Views: 1057

Re: Mosquito wing for objective testing

Seta wrote:
Tue Mar 16, 2021 11:28 am
Thanks for the link, that is a nice brochure
You should absolutely check out ALL the BH and BH2 brochures if you haven't already done so! :D
http://www.alanwood.net/olympus/downloads.html
by viktor j nilsson
Tue Mar 16, 2021 11:03 am
Forum: Microscopes and optics
Topic: Mosquito wing for objective testing
Replies: 18
Views: 1057

Re: Mosquito wing for objective testing

Couple of more question about these magnifiers.. - Do you know if the optical formula changed between BH to BH-2? Can I expect same field coverage/corner quality in APS-C? - Will I be able to use it in conjunction with the PZO MIP5 Head? Olympus catalogues from the BH era suggests that you can use ...
by viktor j nilsson
Tue Mar 16, 2021 8:19 am
Forum: Microscopes and optics
Topic: Has anyone made a visual comparison of Achromatic, Apo and Plan objectives?
Replies: 30
Views: 1756

Re: Has anyone made a visual comparison of Achromatic, Apo and Plan objectives?

I don't know any "definitive reference" on the topic, but there are some interesting comparisons to be found here and there. Different makers has had different standards, and these standards have changed over time - so you can never say anything definitive about such broad classifications. Here are ...
by viktor j nilsson
Tue Mar 16, 2021 5:17 am
Forum: Microscopes and optics
Topic: Mosquito wing for objective testing
Replies: 18
Views: 1057

Re: Mosquito wing for objective testing

viktor j nilsson wrote: ``Getting a DIC intermediate tube seems like an expensive way to get 1.25x! (BH2-NA) Alternatively you could look for a BH2-KP polarizing attachment.`` How do they differ? You mean the dic intermediate tube and the bh2-kp? The dic intermediate tube has, as you know, a slider...
by viktor j nilsson
Mon Mar 15, 2021 12:13 pm
Forum: Microscopes and optics
Topic: Mosquito wing for objective testing
Replies: 18
Views: 1057

Re: Mosquito wing for objective testing

Getting a DIC intermediate tube seems like an expensive way to get 1.25x! Kidding aside, it might be a good idea to get a magnification changer. The BH2-CA has settings for 1x, 1.25 and 1.5x. This can be nice if you want to clip the corners on some objectives (non-plan Fluors) but not others (Plana...
by viktor j nilsson
Mon Mar 15, 2021 10:27 am
Forum: Microscopes and optics
Topic: Mosquito wing for objective testing
Replies: 18
Views: 1057

Re: Mosquito wing for objective testing

Ok, I understand.. :D I am considering getting one of this magnifiers to use it with nikon CFN lenses Olympus performs the corrections on the eyepiece, so I guess this one is neutral guess it is neutral and will work with the nikon lenses via direct projection. That 1.25x is all I would need to rem...
by viktor j nilsson
Mon Mar 15, 2021 8:25 am
Forum: Beginner's corner
Topic: 2 ways to get 200X
Replies: 20
Views: 1212

Re: 2 ways to get 200X

Thanks Pete. My 60x has no field iris, I forgot that many high end objectives do have one. As soon as you said it I felt silly, it was obvious. lol. The field iris would be in the base, below your condenser, not in the objective. The field iris adjusts the size of the illuminated field (in the obje...
by viktor j nilsson
Mon Mar 15, 2021 8:12 am
Forum: Microscopes and optics
Topic: Silicone immersion objectives - any experience?
Replies: 33
Views: 3449

Re: Silicone immersion objectives - any experience?

Hey viktor j Nilsson,came across this post and realised I’ve got the same 100x objective!-I got it mainly for the iris-mine is super smooth for its vintage as well-I haven’t used silicon oil yet but this is the objective that proved to me that oil immersion was something worthwhile! Using oil (hope...
by viktor j nilsson
Mon Mar 15, 2021 12:20 am
Forum: For forum members who want to buy and sell equipment
Topic: Wanted: Leica K2 DIC prism, Nikon L prism
Replies: 26
Views: 9115

Re: Wanted: Leica K2 DIC prism, Nikon L prism

That's great to know, Pete! Did you need to modify the prism at all? The PZO prisms are housed in a removable metal surround. The diameter was OK to fit into an Olympus phase condenser and be secured by the setscrews. The height, however, was too tall and needed to be cut down to a bit under 6mm th...
by viktor j nilsson
Fri Mar 12, 2021 9:21 am
Forum: Microscopes and optics
Topic: Silicone immersion objectives - any experience?
Replies: 33
Views: 3449

Re: Silicone immersion objectives - any experience?

Happy to report that both the 40x 1.00 and 100x 1.25 silicone objectives has now arrived safely and in good condition. Based on the reputation of the grease Olympus used on their classical microscopes, which tends to turn solid with age, I have been rather surprised by how smooth the iris mechanisms...
by viktor j nilsson
Mon Mar 08, 2021 1:47 pm
Forum: Illumination Techniques
Topic: PlasDIC
Replies: 29
Views: 2456

Re: PlasDIC

The two key variables to be optimized is the shear angle and location of the interference fringe. The shear angle is optimized based on the NA of the objective, with narrower angles for high NA/high magnification objectives. I think that it is correct to say that this is because you want the separa...
by viktor j nilsson
Mon Mar 08, 2021 1:33 pm
Forum: Illumination Techniques
Topic: PlasDIC
Replies: 29
Views: 2456

Re: PlasDIC

The two key variables to be optimized is the shear angle and location of the interference fringe. The shear angle is optimized based on the NA of the objective, with narrower angles for high NA/high magnification objectives. I think that it is correct to say that this is because you want the separat...
by viktor j nilsson
Thu Mar 04, 2021 12:55 pm
Forum: Microscopes and optics
Topic: Olympus U-DICT versus U-DICTS
Replies: 18
Views: 1026

Re: Olympus U-DICT versus U-DICTS

I have the DICT-S. I have never noticed any difference in the image quality based on the position of the BFP switch. It looks equally good in either position, at least for my objectives. Some of these require the level in a pushed in position according to the specs. Let me know if you want me to me...
by viktor j nilsson
Wed Mar 03, 2021 9:24 pm
Forum: Microscopes and optics
Topic: Olympus U-DICT versus U-DICTS
Replies: 18
Views: 1026

Re: Olympus U-DICT versus U-DICTS

I believe the condenser prisms has a pin that fits in a groove or hole, which ensures that they are positioned the right way, no?
by viktor j nilsson
Wed Mar 03, 2021 8:28 pm
Forum: Specimens, samples and slides
Topic: PVA-G mountant
Replies: 4
Views: 395

Re: PVA-G mountant

Now that looks very nice and convenient. I would however, be a little hesitant to use this for permanent slides with specimens of potential taxonomic value. Are you planning on donating your collection to a natural history museum eventually? I will donate my fly collection when that time comes, and ...
by viktor j nilsson
Wed Mar 03, 2021 8:13 pm
Forum: Microscopes and optics
Topic: Olympus U-DICT versus U-DICTS
Replies: 18
Views: 1026

Re: Olympus U-DICT versus U-DICTS

Sorry, I'm not sure what you are saying here. This is the situation you are after: If you look at the green box above, note the outward pointing optical axis is on the left not the right as in the original. Technically I guess a rotation would make the green box have the optical axis point in, but ...
by viktor j nilsson
Wed Mar 03, 2021 5:10 pm
Forum: Microscopes and optics
Topic: Olympus U-DICT versus U-DICTS
Replies: 18
Views: 1026

Re: Olympus U-DICT versus U-DICTS

Is rotating the top prism on the vertical axis like this still a valid set up? Come to think of it, the condenser discs can rotate freely, so the top prism, even though it's locked in a slider could rotate that way too. Sorry, I'm not sure what you are saying here. This is the situation you are aft...
by viktor j nilsson
Wed Mar 03, 2021 5:08 pm
Forum: Microscopes and optics
Topic: Olympus U-DICT versus U-DICTS
Replies: 18
Views: 1026

Re: Olympus U-DICT versus U-DICTS

So does the U-DICTS centered knob (unlike the U-DICT screw which moves the prism in and out) rotate the prism? And the side lever moves the assembly in and out? I don't have a U-DICTS slide to check that. I would assume that the centered knob translates the prism in and out, and the side lever lift...
by viktor j nilsson
Wed Mar 03, 2021 7:31 am
Forum: Microscopes and optics
Topic: Olympus U-DICT versus U-DICTS
Replies: 18
Views: 1026

Re: Olympus U-DICT versus U-DICTS

I believe you are correct. It should be looking like this:

Image

I find that schematics of DIC setups are very often drawn inocorrectly.
by viktor j nilsson
Wed Mar 03, 2021 5:33 am
Forum: Microscopes and optics
Topic: Olympus U-DICT versus U-DICTS
Replies: 18
Views: 1026

Re: Olympus U-DICT versus U-DICTS

Could it be that it tilts the prism a couple degrees relative to the optical axis? I remember you posted a link to an Olympus patent on this exact thing: that when you tilt the prism, the location of the Interference fringe shifts, and can thus match objectives with different BFPs. https://i.imgur.c...
by viktor j nilsson
Fri Feb 26, 2021 8:29 pm
Forum: Microscopes and optics
Topic: Back Focal Plan of Olympus BX Objectives
Replies: 7
Views: 419

Re: Back Focal Plan of Olympus BX Objectives

https://www.researchgate.net/post/Is_th ... ctive_lens

I've used the method described by Paolo Pizza with good results. It's much harder at 40x and above.
by viktor j nilsson
Thu Feb 25, 2021 12:15 pm
Forum: Microscopes and optics
Topic: Does it make sense to use Nikon CFN lenses on a olympus BH-2?
Replies: 18
Views: 1302

Re: Does it make sense to use Nikon CFN lenses on a olympus BH-2?

Very nicely done! Good to know that it's possible to do direct projection with the BH-2 trino head. I can't see any reason not to continue with the Nikon CF optics at this point. That is going to be a pretty ideal setup.
by viktor j nilsson
Wed Feb 24, 2021 2:28 pm
Forum: Pictures and Videos
Topic: Blood help identifying cells
Replies: 8
Views: 584

Re: Blood help identifying cells

The post was edited. All is fine now.
by viktor j nilsson
Wed Feb 24, 2021 8:26 am
Forum: Pictures and Videos
Topic: Blood help identifying cells
Replies: 8
Views: 584

Re: Do I need medical attention -My Blood help

Forum rules:
viewtopic.php?f=29&t=4193#p38211

9. No medical advice should be given and posters should not ask for medical advice
by viktor j nilsson
Fri Feb 19, 2021 9:37 am
Forum: Microscopes and optics
Topic: Does it make sense to use Nikon CFN lenses on a olympus BH-2?
Replies: 18
Views: 1302

Re: Does it make sense to use Nikon CFN lenses on a olympus BH-2?

I can see a couple of issues. 1) will you be able to position the sensor at the correct distance for direct projection using the BH-2 trinocular head? It will depend on how easy it is to remove the chimney. I don't remember seeing it being done, but it could just be that since most olympus users use...
by viktor j nilsson
Wed Feb 17, 2021 1:34 pm
Forum: Microscopes and optics
Topic: Stereo monovision
Replies: 13
Views: 691

Re: Stereo monovision

Can you change the diopter setting on both eyepieces? If you can, I would focus on one eyepieces first. Try different diopter settings and see if there is one setting where you maintain parfocality over the entire zoom range, and then adjust the other to match. Are you using glasses? If I'm not usin...
by viktor j nilsson
Sun Feb 14, 2021 5:07 am
Forum: For forum members who want to buy and sell equipment
Topic: Wanted: Leica K2 DIC prism, Nikon L prism
Replies: 26
Views: 9115

Re: Wanted: Leica K2 DIC prism, Nikon L prism

That's great to know, Pete! Did you need to modify the prism at all?
by viktor j nilsson
Sat Feb 13, 2021 8:53 am
Forum: My microscope
Topic: Olympus FHT
Replies: 26
Views: 4627

Re: Olympus FHT

Ask eBay to step in. They will be on your side.
by viktor j nilsson
Thu Feb 11, 2021 9:39 pm
Forum: Microscopy accessories
Topic: Cleanliness of Zeiss 1.5H cover slips
Replies: 21
Views: 2000

Re: Cleanliness of Zeiss 1.5H cover slips

Btw, how do you dry the cover glass after the piranha bath? Multiple washes with deionized water, then submerge the coverlips under ethanol. Whenever I need a coverslip I use tweezers to grab one and let the ethanol evapore on its own, it doesn't leave traces if its sufficiently clean. I see, that ...