MicrobeHunter.com Microscopy Forum

Interesting publication, >4 (9?) mm^2 FOV and is of fully open design - no strings attached. Nice! "The design of the objective is not patented, and it will not be patented in the future. All designs originating in this report are free for reuse, no licensing or material transfer agreements are requ...

Really nice repo: "... contains code for designing, analyzing, and carrying out multicolor structured illumination microscopy experiments based on a digital micromirror device (DMD-SIM), including DMD simulation code, DMD pattern generation, SIM reconstruction and instrument control. It also include...

About the devils and details: Texas Instruments DLP evaluation kits have image offset 100% - for symmetric projection to field diaphragm plane one needs to reposition projection lens and preferably scale the projection image size as necessary. Photographic tilt shift lenses are useful ready made sol...

As expected, someone has already realized quite nice example of optical setup with the DLP4710 EVM:

http://isopack.blogspot.com/2022/03/ima ... scope.html

About the SIM image stack processing - I have yet to try it but there is free to use machine learning code up on Gitlab and a nice 2021 publication going with it: ML-SIM https://github.com/charlesnchr/ML-SIM ML-SIM: universal reconstruction of structured illumination microscopy images using transfer...

Time to sell both and get a really good microscope ?

Try acetone/IPA mix and relatively aggressive scrape with sharpened wooden stick on one edge - if it is residue, it starts to move and there is not much to lose.

Solidified immersion oil?

What considers suitable fluorescence cameras - cheap cooled astronomy cameras seem to be beating 5 year old 12k professional sCMOS camera on readout noise and sensitivity. Sure, relatively low frame rate but this is more the question of sensor resolution. IMX533 camera - cooled, mono and quite remar...

Really nice video on SIM principles, by David Agard: https://www.youtube.com/watch?v=i73HhpLJrqs Basically it is boiling down in information gathering on wavefront, increasing NA with tricks beyond what is possible with just glass shaping in objective. Appears as simple as changing the light source ...

From Leica DMR broshure, it may be the the condenser optical scheme, the massive front element seems quite similar:

Zeiss West Grey WL with complete Jamin-Lebedeff interference contrast set
Where it is located and what you would consider for it?

Still seeking for the Leica 1.4 condenser 11 55 10 04.

At least one lens in the condenser body, not sure if it is cemented doublet or something else.

Multi-lens condenser, this is the top part and at least 3..4 lenses total for the condenser.

How about concave front element condenser?
Relatively modern Leica:

The source of light modulation can also be DLP projector. Check this thread about Aurox and other methods:
viewtopic.php?f=5&t=15272

The SIM light pattern can be projected from DLP projector, just changing out the light source to specific narrow spectral range LED: https://www.nature.com/articles/srep01116 As far I understand, this gives the same properties for high resolution sectioning as the spinning disk pattern. Higher than ...

It seems not to be that complex and has benefits over the classic confocal systems, especially for amateur use with cheaper cameras. There is Zeiss version also, called VivaTome: https://zeiss-campus.magnet.fsu.edu/articles/opticalsectioning/aperturecorrelation/introduction.html Seems a bit over the...

The tech note, how the Aurox system works: https://www.aurox.co.uk/downloads/brochures/Technical%20note%20-%20Optical%20Sectioning%20with%20Structured%20Illumination%20Spinning%20Disc%20Confocal%20Microscopy.pdf If I understand correctly, the processing needed is literally just image differential, t...

Noticed it too late, already bought. If someone here got them and is planning to scan - the third volume is already scanned, available here:
https://openlibrary.org/books/OL1669033 ... microscopy
and also as pdf.

Brings up the question, is that all still too complex for amateur level open source hardware approach - light modulators are reasonably cheap now, the same has pretty much happened to back thinned sensors.

As far as I am understanding, there is no limits but infinity optics and even that may not be strict limit.

I am pretty sure, it is way too expensive but is still interesting... Has anyone used it, any opinions?
https://www.aurox.co.uk/clarity.php

The third volume seems to be available in digital format, has anyone found the second and first in digital?

Well, it always starts as interfere-ometry and in the progress of developed pathology sometimes transits to full blown interferometry.

apochronaut wrote: ↑

Mon Jan 31, 2022 11:58 am

I thought taking objectives apart in the first place WAS interfere-ometry!

It may sound bit grandiose but interferometer and software for analysis is a quite cheap tool nowadays and optical fixtures are not that unreachable devices. Even if original cost effective mass-manufacturing requires relatively high complexity machinery, there is nothing inherently complicated in o...

After DIC analyzer it will not work directly as passive method - the analyzer itself is polarisation filter. It may be possible have additional stereo depth effect if the polarisation angle is slightly rotated, with shuttering periodically one or other eye, offering each eye a little different viewp...

This may have interesting application for stereo modified DIC - slightly shifted polarisation for left/right eye with active shutter, different/multiangle DIC gradient for left/right eye etc.

Boguslaw wrote: ↑

Sun Jan 23, 2022 3:42 pm

A friend has (had?) All three volumes. However, he was supposed to sell them and I don't know if he still has them?

If your friend wants to sell the books anyway, just refer him here - I would be interested in buying.