MicrobeHunter.com Microscopy Forum

Don’t know what to do with this, but sliding two microscope slides together shows interference patterns between the glass at the right angles of light.

I washed them with alcohol so I don’t think it’s an oil layer.

Most likely a similar effect to optical flats.

In metrology, they are used with monochromatic light sources to determine flatness of surfaces to microns. With full spectrum sources, you get the colors.

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As PeteM says ^^^

For a brief introduction; this video is worth a look : https://youtu.be/xSYl7q6yKPU

MichaelG.

Thorlabs uses this technique to mount optics for lapping:

https://www.youtube.com/watch?v=se3K_MWR488

PeteM wrote: ↑

Thu Apr 01, 2021 6:33 am

Most likely a similar effect to optical flats.

In metrology, they are used with monochromatic light sources to determine flatness of surfaces to microns. With full spectrum sources, you get the colors.

Here's an optical flats test I did, checking some optical flats on a first surface mirror (aluminum on glass, metal side touching the optical flat). I remember thinking that I thought the fringing would be stronger. This uses a laser in a ping pong ball:

My slides look like that also-reminds me of soap bubbles...