Permanent fluorescence microscopy slides (letting go)

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bkwoo
Posts: 31
Joined: Tue Feb 18, 2020 1:26 am
Location: Kuala Lumpur, Malaysia

Permanent fluorescence microscopy slides (letting go)

#1 Post by bkwoo » Wed May 26, 2021 1:03 pm

In these difficult and uncertain :roll: pandemic times, I have decided to let go some of my permanent fluorescence microscopy slides to any MH members/hobbyists-amateur microscopists ( rookie or already well-experienced in doing UV fluorescence, spirogyra, acridine orange, etc..) who like to dabble in fluorescence.

Please see attached 1st picture. Sample slides are in middle. :)

You can still view the slides using your usual favourite light technique: DIC, polarized, phase contrast, Rheiberg, etc..and most of the biological entities will be water fleas- moina creatures.

A good chance to boldly go into the wild, uncharted, unexplored frontier of UV microscopy- fluorescence microscopy and to prove that permanentt fluorescence microscopy slides that are 100% photobleach proof do exist.

Interested? Please kindly get in contact with me through this forum (PM here does work, rite? ;) ).

Thank you.

p/s: Who knows..? You may catch better pictures of these pond creatures than my 2nd picture (still having fun messing around with photoshop :) ).
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2nd picture
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"Beautiful is blue" - B.K. Woo

User avatar
bkwoo
Posts: 31
Joined: Tue Feb 18, 2020 1:26 am
Location: Kuala Lumpur, Malaysia

Re: Permanent fluorescence microscopy slides (letting go)

#2 Post by bkwoo » Sat Jul 31, 2021 5:58 am

Story recapped here. The science of fluorescence microscopy vs the diagnostic of fluorescence microscopy vs the art of fluorescence microscopy. All pictures shown here, have been photoshop enhanced.

1st picture: On left, is the science of fluorescence microscopy with the usual F-actin, microtubules and nucleus staining, a domain for cell biologists-professional microscopists, on right is the diagnostic of fluorescence microscopy, a domain for healthcare professionals (pathologists, veterinarians, clinical microbiologists, etc..)- professional microscopists, showing live and dead bacterias.

2nd, 3rd and 4th and 5th pictures: The art of fluorescence microscopy. 4th is UV photomicrography and 5th is UV photomacrography.

2nd picture: Top first column is MCF-7 cells, then E. Coli and others, bottom first column is staphylococcus aureus colonies and the last two are Yakult microbes.

3rd picture: Top left is a Yakult microbe, top right is an unknown microbe which looks like a pac-man, bottom is 2 Yakult microbes, one is being bitten by the other.

2nd and 3rd pictures represent the world of cells and bacterias, for artists-amateur microscopists, your own cheek cells and food bacteria from yoghurt and Yakult drink is your best exploration area.

4th picture is sub-milimetre scale pond creatures of moina and copepod, an excellent domain for artists-amateur microscopists to explore. Not too small like cells and bacterias and not too big either like an ant or a spider and pond water sample are everywhere.

5th picture is mosquito pupa's head, a pond water worm, a damselfly larvae and a black aphid or "water cockcroach"- this small critter runs on water surfaces. Biological entities in 5th picture are generally larger size when compared to 4th picture. A very nice domain for professional photographers who want to explore the world of insects, a shift from the world of animals.

5th picture is best viewed under microscope using temporary microscopy slides with no coverslip. 4th picture can be viewed using both temporary and permanent microscopy slides.

The professional microscopists can cross into amateur microscopy- the artistic pursuit of microscopy, if they want to do so but the artists-amateur microscopists cannot do the job of cells researchers and healthcare professionals. ;)
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"Beautiful is blue" - B.K. Woo

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