aqueous vs. alcohol solutions of stains

[mete]

I think I am confused a bit. I realized some of the stains have both aqueous and alcohol solutions. Is an aqueous solution mainly for wet mounts ? If alcohol (>60%) is used as a fixative, is it better to use an alcohol solution, or the aqueous solution also works equally well ? Does it make sense to rehydrate the sample for staining and then go back to glycerin for mounting ? If the stain has only aqueous solution I guess it is a must ?

[MicroBob]

It would help if you could specify which objects you think about.
Plant sections are mostly stained in water based stains:

Paraffin section
remove paraffin with xylene 2x
isopropanol 2x
ethanol ladder down to water
staining
2-3x isopropanol to remove water
Euparal (very forgiving, isopropanol soluble, tolerant to traces of water)

[mete]

Plant sections and without using paraffin, but I got your point, so if the stain is water based, sample is rehydrated and then stained.