"Permanent" wet mount

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SuiGenerisBrewing
Posts: 78
Joined: Tue Mar 29, 2022 10:39 pm
Location: Canada
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"Permanent" wet mount

#1 Post by SuiGenerisBrewing » Tue Apr 19, 2022 12:59 pm

I am looking for a way to make a permanent "wet mount" of a pond water sample, but I've failed to find a method to do so. The sample will contain a mixture of filamentous algae, diatoms, protists, and bacteria. Would fixing by adding 4% PFA, then allowing the sample to dry to the slide before adding a coverslip and mounting media work?

Thanks

Bryan

DonSchaeffer
Posts: 3274
Joined: Sun Mar 22, 2020 10:06 am
Location: Winnipeg, Manitoba, Canada

Re: "Permanent" wet mount

#2 Post by DonSchaeffer » Tue Apr 19, 2022 2:34 pm

Sounds pretty ambitious.

BramHuntingNematodes
Posts: 1538
Joined: Tue Jan 21, 2020 1:29 am
Location: Georgia, USA

Re: "Permanent" wet mount

#3 Post by BramHuntingNematodes » Tue Apr 19, 2022 2:48 pm

I have some nematodes mounted in glycerol that are sixty years old or so and still show good detail. Try looking for Seinhorst's method.
1942 Bausch and Lomb Series T Dynoptic, Custom Illumination

EYE C U
Posts: 288
Joined: Wed Jul 15, 2020 5:18 pm

Re: "Permanent" wet mount

#4 Post by EYE C U » Tue Apr 19, 2022 5:13 pm

nylon washers.
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