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Xanthum gum (or other thickeners)
Posted: Wed Oct 12, 2022 5:08 pm
by SuiGenerisBrewing
I was wondering if anyone has experimented with "thickening" water-based samples with a thickener like xanthum gum (which doesn't require heating) to partially immobilize subjects? In the "professional" lab we use methylcellulose for this purpose, but at home I have quite a bit of xanthum gum (used for making hotsauce) and no methyl cellulose.
Bryan
Re: Xanthum gum (or other thickeners)
Posted: Fri Oct 14, 2022 3:00 pm
by macnmotion
SuiGenerisBrewing wrote: ↑Wed Oct 12, 2022 5:08 pm
I was wondering if anyone has experimented with "thickening" water-based samples with a thickener like xanthum gum (which doesn't require heating) to partially immobilize subjects? In the "professional" lab we use methylcellulose for this purpose, but at home I have quite a bit of xanthum gum (used for making hotsauce) and no methyl cellulose.
Bryan
Did you ever try using xanthan gum? I'd love to know your results. I have a supply here (also for making hot sauce).
Re: Xanthum gum (or other thickeners)
Posted: Mon Oct 17, 2022 2:25 pm
by SuiGenerisBrewing
Not yet - I'm planning on using it to immobilize a yeast suspension, so that I can do a time-lapse of cell division without the cells floating away. I'd love to get one at 1,000x using phase-contrast!
Re: Xanthum gum (or other thickeners)
Posted: Mon Oct 17, 2022 3:03 pm
by macnmotion
Looking forward to seeing your results. Good luck!