I have been having difficulty sectioning plant tissue; air bubbles in the vessels prevented wax infiltration.
So, thought I would try a subject without air-trapping vessels.
A small earthworm fixed in Bouin's Fluid, dehyrated in alcohol, soaked in xylene, embedded in wax.
Essentially one hollow tube (gut) inside a wider hollow tube (body wall).
10 micron section from a Lipshaw Sledge Microtome; mounted in Euparal
Olympus BH2/BHS, 2x SPlan and D Plan 4x objectives, 1.25x intermediate tube, 2.5x projection eyepiece; Nikon D810
Now to to get some histological stains, likely haematoxylin and eosin; any other recommendations for animal tissue?
Finally - an OK X-section
Finally - an OK X-section
New Brunswick
Canada
Canada
Re: Finally - an OK X-section
Hi Tony,
here you can find a document on paraffin embedding and sectioning worms (in German): http://www.mikrohamburg.de/Tips/Paraffinschnitte_03.pdf
For paraffin infiltration of plant material I use round about this method with isopropanol as intermedium and like the results: http://www.klaus-henkel.de/isomethode.pdf
Attatched som pics on my infiltration and dewaxing setups. I use Paraplast plus (plus as I got it)
Bob
here you can find a document on paraffin embedding and sectioning worms (in German): http://www.mikrohamburg.de/Tips/Paraffinschnitte_03.pdf
For paraffin infiltration of plant material I use round about this method with isopropanol as intermedium and like the results: http://www.klaus-henkel.de/isomethode.pdf
Attatched som pics on my infiltration and dewaxing setups. I use Paraplast plus (plus as I got it)
Bob
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- Entparaffinieren in Versandbehältern 1024.jpg (186.03 KiB) Viewed 2479 times
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- Infiltrieren.jpg (217.29 KiB) Viewed 2479 times
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- Infiltrieren im Kochtopf.jpg (205.45 KiB) Viewed 2479 times
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- Infiltrieren im Kochtopf 2.jpg (164.08 KiB) Viewed 2479 times
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- Entwässern 1024.jpg (146.66 KiB) Viewed 2479 times