Slit Interference Contrast

Here you can discuss DIY adaptations to the microscope.
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lightshape
Posts: 30
Joined: Wed Jan 23, 2019 9:47 pm

Slit Interference Contrast

#1 Post by lightshape » Thu Jan 14, 2021 7:09 pm

Hi,

had to give it a try after reading the PlasDIC post.

No idea about the origin of the Prism i've used, had it around for a while.
Just some tape for the slit to begin.
Tried on a Zeiss Jena Jenamed scope.
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First impressions, slit approx. 3.5 mm, 50x objective.

Open aperture:
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Slit aperture:
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SlitDIC:
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lightshape
Posts: 30
Joined: Wed Jan 23, 2019 9:47 pm

Re: Slit Interference Contrast

#2 Post by lightshape » Thu Jan 14, 2021 7:11 pm

Second impression, slit approx 5.5mm:

Open:
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Slit:
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SlitDIC:
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lightshape
Posts: 30
Joined: Wed Jan 23, 2019 9:47 pm

Re: Slit Interference Contrast

#3 Post by lightshape » Thu Jan 14, 2021 7:32 pm

For comparison, Nomarski DIC
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MicroBob
Posts: 3154
Joined: Sun Dec 25, 2016 9:11 am
Location: Northern Germany

Re: Slit Interference Contrast

#4 Post by MicroBob » Thu Jan 14, 2021 8:21 pm

Hi,
that looks very promising!
Is your setup similar to the PZO DIC with slit condenser? PZO uses a stit aperture in the condenser and an analyser in an intermediate tube between microscope head and observation tube.
Did you see a dependency on the orientation of the diatoms?

Bob

lightshape
Posts: 30
Joined: Wed Jan 23, 2019 9:47 pm

Re: Slit Interference Contrast

#5 Post by lightshape » Thu Jan 14, 2021 9:03 pm

Hi Bob,

the basic design has to be the same as with PZO, but my prism height is fixed.

I didn't focus on details like specimen orientation or perfect rendering yet.
And diatoms are not my favorite specimens for real DIC.
But they look quite nice with slitDIC and i thought they are well suited for a quick comparison and reproducible views.

Using slit aperture and polarizer on their own already greatly improve the contrast of the image.
No news..

Printing an adjustable slit now.

Stefan

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