Diatom id?
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Diatom id?
I'd be interested if anyone can id this quite intricate marine diatom from the Eastern Mediterranean Prepared slide in Pleurax.
40x objective, slightly different views and lighting:
Thanks!
Louise
40x objective, slightly different views and lighting:
Thanks!
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: Diatom id?
A guess - an Amphora species.
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Re: Diatom id?
Oh ok - there seems to be an awful lot of them!
Here's a quick stack I did (8 frames). I'm not very skilled at stacking...
'High' preference
'Low' preference
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: Diatom id?
The excellent resolved stria pattern of image "Do415nmNikon40xCrop100pcScale_00013" is not visible in the stacks. My opinion is that for this diatom, to choose the few images that manifest well focused surfaces and stack them. So I did for a twin of this diatom, albeit I had phase contrast images.
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Re: Diatom id?
I only used Picolay here - Since the end of last year I have Zerene Stacker (on another system) but haven't got around to trying it yet!Hobbyst46 wrote: ↑Tue Apr 06, 2021 3:34 pmThe excellent resolved stria pattern of image "Do415nmNikon40xCrop100pcScale_00013" is not visible in the stacks. My opinion is that for this diatom, to choose the few images that manifest well focused surfaces and stack them. So I did for a twin of this diatom, albeit I had phase contrast images.
I never quite understand how stacking works when you want to see internal structures. I suppose it doesn't, very well! The focus at high NA is critical. I made a little lever to aid adjustment
I'll certainly try again, and as you suggest though my expectations aren't great... The striae patterns were only in more or less one layer, I think, so not many options. But I'll have another go and see what I can do - many thanks!
ps Have you posted pics of your phase contrast version? I'd love to see them
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: Diatom id?
I think that the main differences between stacking softwares is speed, efficiency, user friendliness etc rather than the quality of the final product.
My preferred stacking program is Toupview (free I think). Tab "Process"->"EDF" (extended depth of focus). Superb software in general.
Here is a stack. Although, re-thinking, phase contrast are not ideal for stacking.
P.S. I like the focusing lever !
My preferred stacking program is Toupview (free I think). Tab "Process"->"EDF" (extended depth of focus). Superb software in general.
Here is a stack. Although, re-thinking, phase contrast are not ideal for stacking.
P.S. I like the focusing lever !
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- Posts: 1167
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- Location: Scotland
Re: Diatom id?
Yours is overall much better than mine!Hobbyst46 wrote: ↑Tue Apr 06, 2021 4:29 pmI think that the main differences between stacking softwares is speed, efficiency, user friendliness etc rather than the quality of the final product.
My preferred stacking program is Toupview (free I think). Tab "Process"->"EDF" (extended depth of focus). Superb software in general.
Here is a stack. Although, re-thinking, phase contrast are not ideal for stacking.
P.S. I like the focusing lever !
Picolay is free - written by a German, I think - www.picolay.de
The Zerene Stacker wasn't free!
My hands don't always do quite what I want them to these days The little lever helps. It just clamps on so easy to remove, if necessary.
Cheers
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo