Making glass knives from microscope slides
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Making glass knives from microscope slides
Figure 6-2, Page 78, Plant Microtechnique and Microscopy, Treven E. Ruzin shows how to create glass knives for mictotomes from ordinary microscope slides.
I have attempted this with a friend and we kept getting straight breaks rather than a knife edge.
Has anyone had scussess from making glass knives from miroscope slides? If so can you share your method?
I have attempted this with a friend and we kept getting straight breaks rather than a knife edge.
Has anyone had scussess from making glass knives from miroscope slides? If so can you share your method?
Re: Making glass knives from microscope slides
Very interesting … but no, I have never tried.
I suspect that success might depend on the type [and quality] of the glass.
Another rabbit-hole to explore !!
MichaelG.
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… and explore, I did : finding this useful note from Leica
https://www.leica-microsystems.com/scie ... lications/
MichaelG.
I suspect that success might depend on the type [and quality] of the glass.
Another rabbit-hole to explore !!
MichaelG.
.
… and explore, I did : finding this useful note from Leica
https://www.leica-microsystems.com/scie ... lications/
MichaelG.
Too many 'projects'
Re: Making glass knives from microscope slides
Are you sure you're using the same glass type as described in the book?Gordhandas wrote: ↑Sun Jul 16, 2023 5:00 pmFigure 6-2, Page 78, Plant Microtechnique and Microscopy, Treven E. Ruzin shows how to create glass knives for mictotomes from ordinary microscope slides.
I have attempted this with a friend and we kept getting straight breaks rather than a knife edge.
Has anyone had scussess from making glass knives from miroscope slides? If so can you share your method?
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Re: Making glass knives from microscope slides
I have done this a lot. However, I have always used glass strips specifically made for EM ultra microtomy. You first cut a square and then divide it half to make two triangles. You may use an ordinary glass knife and pliers to get a reasonable good quality knife for light microscopy.
There seems to be those stripes in ebay e.g. https://www.ebay.com/itm/402979555070?h ... R7Tm3sytYg
As far as I know the glass quality is important (hardness and the way they have been casted).
There seems to be those stripes in ebay e.g. https://www.ebay.com/itm/402979555070?h ... R7Tm3sytYg
As far as I know the glass quality is important (hardness and the way they have been casted).
Re: Making glass knives from microscope slides
A long time ago I saw a university researcher make microtome knives by whacking a piece of plate glass with a hammer. It shattered into many pieces, several of which had very thin and sharp edges.
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Re: Making glass knives from microscope slides
Has anyone had scussess from making glass knives from miroscope slides? If so can you share your method?
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Are you sure you're using the same glass type as described in the book?
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The book says microscope slides and have tried it with Soda glass and Borosilicate glass.
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Are you sure you're using the same glass type as described in the book?
[/quote]
The book says microscope slides and have tried it with Soda glass and Borosilicate glass.
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- Posts: 10
- Joined: Sun Feb 27, 2022 10:08 am
- Location: Yorkshire, UK
Re: Making glass knives from microscope slides
Many thanks for that. A friend who has made glass knives for Electron Microscopes tells me the best way is to drop a whole box of slides and shatter them all. Then look for the ones with the best edge profile. Place glass idea is another one to try, perhaps with greenhouse glass; easy to hold off and cheap enough. Thanks for the idea.