Imaging Diatoms RBGE

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75RR
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Imaging Diatoms RBGE

#1 Post by 75RR » Wed Nov 25, 2020 10:03 am

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Repeating here to facilitate locating it. viewtopic.php?f=9&t=11121

Imaging Diatoms (Royal Botanic Garden Edinburgh) for the ADIAC Diatom Image Database

https://www.rbge.org.uk/collections/online-resources/

https://websites.rbge.org.uk/ADIAC/pubdat/prepro.html

Interestingly they use Brightfield, though I think that COL would probably serve them better.

"All diatoms are photographed using brightfield optics only. There are illumination systems that offer better contrast (phase, and differential interference contrast)¸
but it was decided not to use these since they introduce artifacts into the image that are very difficult to standardize;
DIC introduces artificial shading giving the impression of directional illumination, i.e. the diatom outline looks quite different depending on the direction of the illumination."
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

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