AO Series 10 - serial 824061

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dtsh
Posts: 977
Joined: Wed May 01, 2019 6:06 pm
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AO Series 10 - serial 824061

#1 Post by dtsh » Tue Apr 12, 2022 4:30 am

This is an American Optical Series 10, Serial number 824061

For those not familiar with this series allow me a moment to prattle on about it. I admit up front that I am quite fond of the platform so there is a bias to be aware of, but I think most will agree that not only are these quite nice instuments, you'll be hard-presssed to find anything comparable anywhere near this price.

In 1961, AO decided to focus their efforts on infinity corrected optics and introduced their 34mm parfocal infinity system at a time when the rest of the industry was focused on using finite tube lengths such as the common 160mm TL microscope. It’s hard to discuss some of the features without an understanding of infinite vs finite, but I’m only going to cover some of the very basic concepts. In an infinity system, the light bundle that comes out of the back of the objective is effectively parallel and it uses a telan lens in the base of the head to convert that parallel bundle into a beam that converges to a focus to be picked up by the eyepieces. What this means is that, theoretically the objective and the head can have a variable distance between them and still work as a complete system delivering a useful image at the correct magnification. This might not seem especially useful at first, but it allows adding various analyzers and other instruments into the path without affecting the focus of the final image, a feature that finite systems struggle with, making the same product capable of a variety of advanced techniques easily by adding modules to extend functionality. There were a lot of these made with a variety of add-ons allowing brightfield, epi illumination, darkfield, phase contrast, fluoresence microscopy, and more just by adding or swapping out components.

An additional feature that’s easy to overlook at first glance is that this allows changing the way focus is achieved. With the 34mm infinity system, AO was able to alter the design so that adjusting the focus raises and lowers the nose instead of the stage while the head remains fixed. This might seem trivial, but it allows something few other systems could achieve; effectively removing the possibility of crushing a slide and objective together and destroying one or both as the nose simply comes to rest on top of the slide instead of being driven into it. Another added benefit is the ability to quickly change objectives and cycle through uniform slides while maintaining focus, requiring only minor adjustment of the fine focus to account for the minor deviations. Here’s an excerpt from the manual, describing the process.

“The autofocus stop is pre-set at the factory. It is the stop which limits the height of the stage. This setting is valid for slides anywhere from 0.50 to 2.5 mm, in thickness. Because of the highly precise tolerances held in the manufacture of the objectives, it is possible to search the slide with 10x or 45x objectives, find the required field for study under oil immersion, raise the coarse adjustment, swing to the oil immersion objective, apply oil to slide, and lower the coarse adjustment rapidly to its positive stop. The subject will be in focus within a touch of the fine adjustment with absolutely no danger of touching the slide.”

This leads to a discussion of the focus mechanism which I personally consider beautiful in it’s simple elegance. Here’s a link to the AO 10 manual (there are other versions of the manual at PS Neeley’s):
https://user.xmission.com/~psneeley/Per ... ersion.pdf

Unlike some manuals, the AO 10 manual is a pretty good resource for getting familiar with using the instrument and is worth a read. It is not a repair manual nor is it an introduction to microscopy, but it does contain exploded parts diagrams which can be quite useful, such as this one regarding the focus which I was just discussing:
AO10_focus1.jpg
AO10_focus1.jpg (94.26 KiB) Viewed 1668 times

If you study the above image, you’ll see that it is effectively a very large screwform. Within the thread form of the coarse focus rides a ball that is connected to an arm which translates lateral motion of the ball into vertical motion of the arm upon which the nose and objectives are mounted, thus raising or lowering the head as the ball moves from one side to the other. A look at the parts diagram in the back of the manual shows the larger screwform with a smaller and finer screw inside it; the larger is the coarse focus and the smaller, the fine. Rotation of the fine focus moves the block which is the larger coarse focus laterally. Of note, the arm and ball are loaded by gravity; this permits largely slop-free focus and reduces the risk of damaging slides or objectives by accidentally crushing them together. That later being a feature that should stand out to anyone who’s accidentally crushed an expensive/loved slide under an objective.

This series are well built quality microscopes, usual problems with these are frozen greese in the head and focus mechanism, but this one has been cleaned and relubed so that is not a concern.

This series uses AO's 34mm parfocal infinity objectives, a product line AO used for many years over a variety of models. This particular scope is equipped with a 4x/0.12NA Cat.1017 plan achro, 10x/0.25NA Cat.1019 plan achro, 45x/0.66NA Cat.1116 achromat, and a 100x/1.25NA Cat.1079 achromat. For those not familiar, plan objectes provide a "flat field" over most of the field of view where an achromat that isn't plan will be in focus at the center, but the periphery will become more and more out of focus.

This unit comes with a 1.25NA Abbe condenser Cat.1088 with an optional swing-in auxilliary lens to fully illuminate the field for the 4x objective. Of note, the swing-in aux lens is NOT the original AO one, but rather a new replacement which I created detailed here:
viewtopic.php?p=120232#p120232


The stage is the standard mechanical stage. The focus knobs are marked with 1 micron increments, which provides measurement in the Z axis.

The illuminator is the Cat.1036, 6V 17watt tungsten lamp; it does not have a field iris as the Cat.1036A does, so full Koehler illumination is not possible without replacing the illuminator. The 1036 illuminator does include a built in neutral density filter (10%) to cut the light intensity more, which is nice for low power viewing. Included is a Cat.1051 transformer to provide variable voltage to the lamp.

The head is a binocular, trinocular heads are easy to install and typically not difficult to find, but I don't have any more spares so cannot offer the option.

The scope comes with a pair of 10x Cat.176 eyepieces. A pair of 15x Cat.184 eyepiece can be included for an extra $35.00

This microscope is used and shows signs of wear and has a few nicks and blemishes as one would expect from an instrument that's 50 years old. It's in good serviceable shape and everything works as it's supposed to. I believe in the value of these scopes enough to offer that if you aren't happy with it, you have 60 days to send it back and I'll refund your money.

I always throw in a few extras, a box of KimWipes, a litle 3D printed holder for 32mm filters, a 3D printed eyepiece case, and a poorly made slide of some Chrysops fly parts (the same slide used for the images).

Now for the images....
AO10_1.jpg
AO10_1.jpg (178.7 KiB) Viewed 1668 times
AO10_2.jpg
AO10_2.jpg (112.67 KiB) Viewed 1668 times
AO10_3.jpg
AO10_3.jpg (105.64 KiB) Viewed 1668 times
AO10_4.jpg
AO10_4.jpg (138.18 KiB) Viewed 1668 times

dtsh
Posts: 977
Joined: Wed May 01, 2019 6:06 pm
Location: Wisconsin

Re: AO Series 10 - serial 824061

#2 Post by dtsh » Tue Apr 12, 2022 4:34 am

Some images of a section of buttercup stem

4x plan achromat objective
Buttercup_stem_40x.jpg
Buttercup_stem_40x.jpg (87.29 KiB) Viewed 1667 times
10x plan achromat objective
Buttercup_stem_100x.jpg
Buttercup_stem_100x.jpg (108.84 KiB) Viewed 1667 times
45x achromat objective
Buttercup_stem_450x.jpg
Buttercup_stem_450x.jpg (103.55 KiB) Viewed 1667 times
100x achromat objective
Buttercup_stem_1000x.jpg
Buttercup_stem_1000x.jpg (91.6 KiB) Viewed 1667 times

dtsh
Posts: 977
Joined: Wed May 01, 2019 6:06 pm
Location: Wisconsin

Re: AO Series 10 - serial 824061

#3 Post by dtsh » Tue Apr 12, 2022 4:38 am

I deal with tabanid flies often enough that a slide with a Chrysops (deerfly) wing is ever-present

4x plan chromat objective
Chrysops_wing_40x.jpg
Chrysops_wing_40x.jpg (104.42 KiB) Viewed 1667 times
10x plan achromat objective
Chrysops_wing_100x.jpg
Chrysops_wing_100x.jpg (104.65 KiB) Viewed 1667 times
45x achromat objective
Chrysops_wing_450x.jpg
Chrysops_wing_450x.jpg (154.03 KiB) Viewed 1667 times
100x achromat objective
Chrysops_wing_1000x.jpg
Chrysops_wing_1000x.jpg (70.79 KiB) Viewed 1667 times
For specimens with depth, it's difficult to get a good image at high magnifications without stacking, but I felt stacking wouldn't be appropriate.

dtsh
Posts: 977
Joined: Wed May 01, 2019 6:06 pm
Location: Wisconsin

Re: AO Series 10 - serial 824061

#4 Post by dtsh » Tue Apr 12, 2022 4:41 am

The Flea was a bit too deep in the mountant to get an acceptable image with the 100x objective, so that objective is left out.

4x plan achromat objective
Flea_40x.jpg
Flea_40x.jpg (51.87 KiB) Viewed 1667 times
10x plan achromat objective
Flea_100x.jpg
Flea_100x.jpg (57.65 KiB) Viewed 1667 times
45x achromat objective
Flea_450x.jpg
Flea_450x.jpg (59.29 KiB) Viewed 1667 times

dtsh
Posts: 977
Joined: Wed May 01, 2019 6:06 pm
Location: Wisconsin

Re: AO Series 10 - serial 824061

#5 Post by dtsh » Tue Apr 12, 2022 4:45 am

The classic onion root tip. Of the images thus far, this sample begins to do justice with the 100x achromat.

4x plan achromat
Onion_root_40x.jpg
Onion_root_40x.jpg (57.97 KiB) Viewed 1667 times
10x plan achromat
Onion_root_100x.jpg
Onion_root_100x.jpg (79.65 KiB) Viewed 1667 times
45x achromat
Onion_root_450x.jpg
Onion_root_450x.jpg (181.52 KiB) Viewed 1667 times
100x achromat
Onion_root_1000x.jpg
Onion_root_1000x.jpg (116.25 KiB) Viewed 1667 times

dtsh
Posts: 977
Joined: Wed May 01, 2019 6:06 pm
Location: Wisconsin

Re: AO Series 10 - serial 824061

#6 Post by dtsh » Tue Apr 12, 2022 4:55 am

I realize this is posted to the buy/sell section and there's no price! All part of the design...I was asked what I had available and this is it for now, no price as the individual who asked can accept or pass, if they choose to pass I'll add a price for anyone else who might be interested.

But do please ask questions and point out flaws to help anyone interested in any AO10, or this one specifically, make an informed decision.

I mentioned it early on, but I want to reiterate it again, the condenser aux lens installed is NOT an original AO one, it's one I designed, printed a housing for, and sourced a lens to be a close match for the original. In my testing, I can't see any difference between the original and my replacement, except to say that the replacement does not perform acceptably with an AO 2.5x objective (which is not included).

I didn't work terribly hard to capture the images nor to process them much more than the basics as I wanted to show it as it appears to the eye as honestly as possible.

The two main pain points with a series 10, frozen grease in the focus and eyepiece diopter adjustment, have been cleaned and relubed so that both are in good working order; as is the rest of the instrument.

I have a few other slides I could image, including an arranged diatom test slide I've posted images of before. If anyone wants more images, ask and I'll see what I can do.
Imaging was done with my usual setup of a Raspberry Pi HQ camera with a c-mount lens, mounted afocally to capture the image through an eyepiece.

dtsh
Posts: 977
Joined: Wed May 01, 2019 6:06 pm
Location: Wisconsin

Re: AO Series 10 - serial 824061

#7 Post by dtsh » Thu Apr 14, 2022 10:55 pm

Hopefully this will be helpful to the new owner or perhaps another owner one in another 50 years.

fjordwarrden
Posts: 16
Joined: Mon Mar 28, 2022 5:55 pm
Location: United States, Santa Cruz California

Re: AO Series 10 - serial 824061

#8 Post by fjordwarrden » Fri Apr 15, 2022 2:46 am

Very helpful!

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