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Laminar flow equipment
Posted: Fri Feb 02, 2024 9:08 am
by Frankiev
The subject of tissue culture intrigues me and I'm contemplating buying / making a laminar flow hood or cabinet. Does anyone have any experience in this field?
Realistically I would only be interested in UK based suppliers. I'm pretty experienced in a wide range of DIY procedures.
Thanks
Re: Laminar flow equipment
Posted: Fri Feb 02, 2024 3:46 pm
by Tom Jones
Make sure you're sterile technique is up to speed. Tissue culture is pretty unforgiving when it comes to viral or bacterial contamination.
Re: Laminar flow equipment
Posted: Fri Feb 02, 2024 5:27 pm
by SuiGenerisBrewing
Your best bet for DIY option would be one of the HEPA laminar flow systems mushroom growers use. These produce a continuous stream of sterile air that you can work in. Downside is they only protect your samples (and actually blow your samples into your face), so you have to be cautious of what you are culturing. An example build, to give you an idea what is entailed:
https://learn.freshcap.com/growing/keeping-it-clean-how-to-design-and-build-a-laminar-flow-hood/
Re: Laminar flow equipment
Posted: Fri Feb 02, 2024 10:10 pm
by Frankiev
Thanks for your input.
Re: Laminar flow equipment
Posted: Thu Feb 29, 2024 10:30 pm
by BadDog
I am planning to build one of these.
https://www.patreon.com/posts/78502280
for eventual mushroom propagation (and agree with SuiGenerisBrewing on what you might be breathing in)
Meanwhile, I think it can help me when I am removing dust from optics
I am not so concerned about perfectly laminar flow as long as it is relatively dust free.