reichert jung microstar 110 fluorescence

What equipment do you use? Post pictures and descriptions of your microscope(s) here!
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maguee
Posts: 22
Joined: Tue Feb 15, 2022 9:23 am

reichert jung microstar 110 fluorescence

#1 Post by maguee » Tue Nov 28, 2023 5:34 pm

I can do blue excitation light. But i would like to see the red autoflorescence of vegetal stuff.

I use a RGB LED torch that works wonderfully but i have two diferent position for the cube filter and i think that none of them allows for that. Should i try to change the filters in the cube?

I think blue and Green excitation are the most common.

Hobbyst46
Posts: 4267
Joined: Mon Aug 21, 2017 9:02 pm

Re: reichert jung microstar 110 fluorescence

#2 Post by Hobbyst46 » Wed Nov 29, 2023 11:44 am

maguee wrote:
Tue Nov 28, 2023 5:34 pm
I can do blue excitation light. But i would like to see the red autoflorescence of vegetal stuff.

I use a RGB LED torch that works wonderfully but i have two diferent position for the cube filter and i think that none of them allows for that. Should i try to change the filters in the cube?

I think blue and Green excitation are the most common.
For effective plant autofluorescence one needs to excite at 400-430nm (that is violet, not blue), cutoff (dichroic mirror) around 500nm, end an emission filter that transmits above 550-600nm.

jorymil
Posts: 44
Joined: Tue Dec 05, 2023 5:06 am

Re: reichert jung microstar 110 fluorescence

#3 Post by jorymil » Tue Jan 16, 2024 9:27 am

maguee wrote:
Tue Nov 28, 2023 5:34 pm
I can do blue excitation light. But i would like to see the red autoflorescence of vegetal stuff.

I use a RGB LED torch that works wonderfully but i have two diferent position for the cube filter and i think that none of them allows for that. Should i try to change the filters in the cube?

I think blue and Green excitation are the most common.
Were you able to work this out? I know that UV flashlights often contain some violet in them if you're looking for an inexpensive light source. Though you definitely will want some filtering in place. I've never seen a violet-specific flashlight in a hardware store, but the UV ones are really common.

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