Coloured Backgrounds

[Peter-J]

Could you tell me how you get the coloured backgrounds. I’m new to this and just bought a new microscope.

[Alexander]

Photoshop is your friend.

[viktor j nilsson]

Robert is pretty generous with sharing details about his photos. On the page below, you'll see that he used DIC illumination for that photo:
www.canadiannaturephotographer.com/ciliates.html

Buying a DIC capable microscope is not cheap, usually a couple thousand USD.

A fairly similar color scheme can be achieved more affordably using polarization + a full wave plate. Robert uses this technique on many of his other more colorful photos. To do this, you just need two linear polarizers, one below the condenser and one above the objective, and a full wave plate somewhere. This can easily be DIY'd with almost any microscope.

[Greg Howald]

It can be achieved using a rheinberg filter. This filter has a colored center spot the same size as that used for darkfield, and and outer ring of a different color. The spot should always be a darker color than the outer ring.
The spot determines background color while the outer ring determines the color which enhances the specimen. I suggest you look up rheinberg filters on you tube to get the details on how to do it. Results can be dazzling using rheinberg or obliques filters that fit in the filter holder under the condenser. Oliver Kim has a you tube video concerning oblique filters.
Greg

[Plasmid]

Using polarizer, analyzer and lambda plate

https://youtube.com/shorts/Q0RR109LsCw?feature=share

[Alice.hp]

I'm inerested in it too. Thanks for sharing