If I get a more powerful light I could have a larger depth of field? Having more light when closing the iris?
Thanks
Light power and DoF
Re: Light power and DoF
The answer ist no. Closing own the iris will reduce resolution.
There is a hard trade-off between high NA (high resolution and low field of depth) and low NA (low resolution with high field of depth).
There is a hard trade-off between high NA (high resolution and low field of depth) and low NA (low resolution with high field of depth).
Re: Light power and DoF
Ok, I understand. Thanks
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Re: Light power and DoF
It my reduce resolution but it gives you so much more depth of field that you can see things that are otherwise not visible. You sometimes have to lower light levels (I use software) to make things visible.
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Re: Light power and DoF
NA = numerical aperture
Much more detail from the fine folks at Nikon here:
https://www.microscopyu.com/microscopy- ... ld-of-view
Best Regards,
Mike M.
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Michael E. Marotta
Technical Writer
Michael E. Marotta
Technical Writer