locating Amoebas on my slides
locating Amoebas on my slides
I’m having difficulty locating Amoebas on my slides (40x,100x, 400x) because of their transparency and no definitive shape. I have no difficulty with any other specimens. I appreciate any advice.
Re: locating Amoebas on my slides
Some method of adding contrast will help. Phase contrast, darkfield, oblique, DIC, etc.
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Re: locating Amoebas on my slides
Yeah, oblique illumination is probably the simplest and easiest way to show up protists generally. You also need to be fairly certain that you actually have some! Here are some useful tips https://www.microscopemaster.com/amoeba ... scope.html
Louise
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: locating Amoebas on my slides
Thanks, I'll let you know how I make out.
Re: locating Amoebas on my slides
In my opinion, if it can not be found through bright field illumination (reducing the grating aperture), any other means to increase the contrast can do nothing.
viewtopic.php?f=13&t=15675
viewtopic.php?f=13&t=15675
Micrographers from China, thanks to the forum for providing a platform for exchange
Re: locating Amoebas on my slides
I hadn't realised I had a slide full of amoeba until I happened to be playing about with crossed polarisers. Even at low power the amoebas jump out at you, glittering brightly as the crystals in the cytoplasm flow around the cell. I now use this method routinely to check for amoeba, I recommend you try it.
If your scope is not set up with polarisers one over the field diaphragm and one over the eyepiece should work, rotate either to extinction. If they're photographic polarisers first check which way up they need to be to get proper extinction.
If your scope is not set up with polarisers one over the field diaphragm and one over the eyepiece should work, rotate either to extinction. If they're photographic polarisers first check which way up they need to be to get proper extinction.