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Almost all my images benefit from sharpening - I'm using GIMP photo editor. The two images below are a rather extreme example, but the blurry image is much improved by sharpening, without any apparent (to me) degradation. Subject in Hydropsychidae larva. The details derived by the sharpening process must be present in the original image. Is there some adjustment to be made in the camera configuration that would accomplish the sharpening up-front? Is there a reason this is not desirable? Would more or fewer pixels help? Maybe higher ISO speed? I have 2 mirrorless cameras - Olympus Pen-E PL7 and Canon M200, and they both behave similarly in this regard. Thanks.original sharpened
In extreme macro and micro we are always near to the diffraction limit relatively speaking, not much we can do about that (nothing at all past a point). But we know diffraction blurs things, so by subtracting a blurred version of your image from the image you can partially reverse it. It seems dumb but it works (blind deconvolution).
May i recommend the use of registax? Its intended mainly for planetary astrophotography, but ive used it for correcting CA, color correction, and some sharpening, on my microphotography.