why are my photo's and video's always yellowish?

Here you can discuss different microscopic techniques and illumination methods, such as Brightfield, Darkfield, Phase Contrast, DIC, Oblique illumination, etc.
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janvangastel
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why are my photo's and video's always yellowish?

#1 Post by janvangastel » Wed Jan 08, 2020 9:32 am

My photographs and video's always have a yellowish/brown background, which I do not see in other footage. This is even the case with clear water between the specimen glasses and relatively independent of the brightness of the light I use. See attached photograph (sharpened too much, but this photo is only to illustrate my point). Does everyone use a blue filter or something? It could be the halogen lamp (of which I tried different brands), but I can't find other colors then 'warm white'. I use a Panasonic C9 camera and an adapter with 50% relay lens. I don't think the camera is the problem, because the bird photographs I take are fine.
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MichaelG.
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Re: why are my photo's and video's always yellowish?

#2 Post by MichaelG. » Wed Jan 08, 2020 10:48 am

Sorry, I can find no reference to a Panasonic C9 camera
... Do you mean the Panasonic DC G9 :?:

Whatever ... It should be simply a matter of selecting/adjusting ‘White Balance’
[this can be done either in camera, or in post-processing]

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MicroBob
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Re: why are my photo's and video's always yellowish?

#3 Post by MicroBob » Wed Jan 08, 2020 10:54 am

Hi Jan,
halogen light has a yellow tint and often is used with a blue filter on top of the field iris.
Camera light meters expect an object of the brightness of an 18% grey card. In bright field microscopy you have a lot of bright background and your little object. To get a good exposure you have to use exposure compensation like +1 or +2 stops.
After the shot you can set your white point on the area you want white. But first you should optimize the exposure.

Bob

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janvangastel
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Re: why are my photo's and video's always yellowish?

#4 Post by janvangastel » Wed Jan 08, 2020 12:59 pm

Do you mean the Panasonic DC G9
Sorry, I meant Panasonic Lumix G9.
Thanks for the answers. I will correct my exposure and take look at the white balance. And buy a blue filter.

MichaelG.
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Re: why are my photo's and video's always yellowish?

#5 Post by MichaelG. » Wed Jan 08, 2020 2:22 pm

It looks to be a superb camera : https://camerajabber.com/panasonic-g9-review/

I'm sure that the White Balance setting will do what's needed.

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RobBerdan
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Re: why are my photo's and video's always yellowish?

#6 Post by RobBerdan » Mon Jan 20, 2020 5:18 am

Re yellow photos - you can do one of four things to fix the background colour:

1) use a blue filter - they are sometimes called daylight filters, and use the max brightness of your bulbs
2) You can set you camera white balance to tungsten about 3200 K
3) You can fix the colour quickly if you use Photoshop - in camera RAW there is a white balance tool, click on a gray area and the background will become white with equal RGB numbers . This requires post processing with software and provides the best result
4) You can use a daylight LED light for your microscope they make them for a few hundred dollars and sell them on ebay - I have purchased two of them for older microscopes.
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janvangastel
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Re: why are my photo's and video's always yellowish?

#7 Post by janvangastel » Mon Jan 20, 2020 7:45 am

Thanks for the answers. I have changed the white balance and bought a blue filter. Already much better, but I am still searching for the optimum setup.

apochronaut
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Re: why are my photo's and video's always yellowish?

#8 Post by apochronaut » Mon Jan 20, 2020 4:26 pm

RobBerdan wrote:
Mon Jan 20, 2020 5:18 am
Re yellow photos - you can do one of four things to fix the background colour:

1) use a blue filter - they are sometimes called daylight filters, and use the max brightness of your bulbs
2) You can set you camera white balance to tungsten about 3200 K
3) You can fix the colour quickly if you use Photoshop - in camera RAW there is a white balance tool, click on a gray area and the background will become white with equal RGB numbers . This requires post processing with software and provides the best result
4) You can use a daylight LED light for your microscope they make them for a few hundred dollars and sell them on ebay - I have purchased two of them for older microscopes.
A slide that has been stained with toluidine blue? not the best example of how to beat the yellows.

pigboy1537
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Re: why are my photo's and video's always yellowish?

#9 Post by pigboy1537 » Wed Feb 19, 2020 10:18 pm

The light that are used on microscopes has a yellowish tin to it. If you White balance your camera it will make it proper color.

Sure Squintsalot
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Re: why are my photo's and video's always yellowish?

#10 Post by Sure Squintsalot » Fri Jun 24, 2022 5:02 pm

Light color is a function of filament temperature: as you dim your light, the filament gets cooler and redder and gets whiter and hotter with increased brightness.

A single blue filter will only work for a specific range of brightness; on either side of that range, you'll need different blue filters.

Your camera has a range of white balance adjustments, but those will NOT change as a function of filament temperature. You'll have to readjust white balance every time you dim or amplify lighting. It'll be a constant dance of white balance adjustment and blue filter swaps ever time you adjust your lighting.

The modern LED light is a godsend in this respect. A high quality LED light will provide white light regardless of intensity and you can customize, "set and forget", your camera's white balance setting once you have it dialed in.

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imkap
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Re: why are my photo's and video's always yellowish?

#11 Post by imkap » Sun Jun 26, 2022 11:12 am

You can shoot in RAW and adjust white balance in post processing.

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