PlasDIC questions

Here you can discuss different microscopic techniques and illumination methods, such as Brightfield, Darkfield, Phase Contrast, DIC, Oblique illumination, etc.
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microscopeboi
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Joined: Wed Apr 15, 2020 11:26 pm

PlasDIC questions

#1 Post by microscopeboi » Tue Feb 09, 2021 2:12 pm

hello, ive been looking into plasdic, as i think i will soon be ready to purchase a prism or two. So, from my understanding, rather than using the analyzer prism you replace it with a 'slit' patch stop and the length/width of the slit effects the amount of contrast? additionally, i understand that you can use this, or varying dimensions of this, to replace all need for analyzer prisms, is this true? if so, what would be the difference between a lower NA slit and a higher NA slit? Additionally, ive read that plasdic doesnt really work for higher (<.65 NA) objectives, could the combination of oblique and plasDIC be used to compensate for a higher NA? lastly, if not, does anyone know how to get it to work at higher NA's? Thanks!

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