Brand New Amscope Phase Contrast Kit

Here you can discuss different microscopic techniques and illumination methods, such as Brightfield, Darkfield, Phase Contrast, DIC, Oblique illumination, etc.
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PeteM
Posts: 2983
Joined: Tue Dec 06, 2016 6:22 am
Location: N. California

Re: Brand New Amscope Phase Contrast Kit

#31 Post by PeteM » Mon Mar 29, 2021 12:26 am

Great thread Red-Green. Thanks. Your pictures got better and better - and the examples provide others with a good idea of what phase contrast adds for near-transparent specimens.

chrisimbee
Posts: 105
Joined: Fri Mar 19, 2021 5:34 pm

Re: Brand New Amscope Phase Contrast Kit

#32 Post by chrisimbee » Fri Apr 16, 2021 2:12 pm

OMG ; this phase contrast kit is amazing !!! I feel like buying it too!!
Thanks for sharing these fantastaic photos!!

I have a question : what diameter is the condenser please ?

Chris
microscope Olympus BH2-BHTU+epifluo RFC @ 470 nm
Zeiss neofluar x16Ph, x40Ph, x100 oilPh
LOMO Ph x10 X20 X40 X90oil
Olympus SPFl x2
Olympus SPx20, SPx40 SPx100
camera astro ZWO ASI 120MM (n&b) et ZWO ASI 120 MC (colour)
Nikon D3100

Red_Green
Posts: 126
Joined: Fri Jun 05, 2020 9:38 pm

Re: Brand New Amscope Phase Contrast Kit

#33 Post by Red_Green » Mon Apr 19, 2021 4:40 pm

chrisimbee wrote:
Fri Apr 16, 2021 2:12 pm
OMG ; this phase contrast kit is amazing !!! I feel like buying it too!!
Thanks for sharing these fantastaic photos!!

I have a question : what diameter is the condenser please ?

Chris
I believe the Mounting size of condenser is 37mm in diameter / bottom mount.

The only cons that I have really found with this phase kit is that the phase plates on the turret sometimes become unaligned especially when switching between darkfield / brightfield and that each one is just adjusted with your fingers. So every so often I have to realign. Not really a big deal, just a slight annoyance.

Other that, it's a pretty amazing kit. The pictures and view that you get from it is outstanding.

chrisimbee
Posts: 105
Joined: Fri Mar 19, 2021 5:34 pm

Re: Brand New Amscope Phase Contrast Kit

#34 Post by chrisimbee » Wed Apr 21, 2021 4:45 am

thanks a lot :)
microscope Olympus BH2-BHTU+epifluo RFC @ 470 nm
Zeiss neofluar x16Ph, x40Ph, x100 oilPh
LOMO Ph x10 X20 X40 X90oil
Olympus SPFl x2
Olympus SPx20, SPx40 SPx100
camera astro ZWO ASI 120MM (n&b) et ZWO ASI 120 MC (colour)
Nikon D3100

User avatar
woyjwjl
Posts: 325
Joined: Tue Jan 04, 2022 1:55 pm
Location: Wuhan, China

Re: Brand New Amscope Phase Contrast Kit

#35 Post by woyjwjl » Tue Feb 22, 2022 7:37 am

IMHO, the PC kit effect is sorry for the price, and the effect produced by one finger (oblique lighting) is enough to surpass it.
Micrographers from China, thanks to the forum for providing a platform for exchange

MicroscopyUniverse
Posts: 60
Joined: Wed Oct 13, 2021 8:34 am

Re: Brand New Amscope Phase Contrast Kit

#36 Post by MicroscopyUniverse » Wed Feb 23, 2022 11:17 pm

Hi Red_green,

In post #13, the olympus 40x picture shows too oblique illumination. Look at the upper left part of the picture. Bacteria
appear with too much 3d effect. This may be a illumination issue, or light and optics alignment problem which can destroy
the phase contrast. For my opinion, this picture does not seem to have a correct phase contrast setup or something
is wrong.

In my job, I use phase contrast daily for live cells using equipment from different brands (olympus of course and nikon and leica as well) and the result is clearly better than the one showed by the mentioned olympus 40x picture. I don’t know what equipment
is used in this picture and may be interesting to know.

On the other hand, the results obtained with your Amscope kit are very good and for all those for whom DIC is out of they budget,
phase contrast will be a new world to discover.

Thanks for sharing

Sansub2
Posts: 101
Joined: Wed Oct 20, 2021 3:34 pm

Re: Brand New Amscope Phase Contrast Kit

#37 Post by Sansub2 » Thu Feb 24, 2022 1:18 pm

Is there different type of phase kits available? The one I have only produce gray background at the most, not dark and not at this level of contrast. There is no halo around any subject. Yes, it is centered correctly using phase telescope. It is a positive phase objectives (PHP written).

Some of the images in this post are excellent.

Scoper
Posts: 153
Joined: Tue Jan 24, 2023 4:06 pm

Re: Brand New Amscope Phase Contrast Kit

#38 Post by Scoper » Fri Jun 30, 2023 4:19 pm

Does anyone else have any other experience with the Amscope phase kit for the 490 series of microscope?

And how it compares to other brands?

Thanks

paulg269
Posts: 9
Joined: Wed Apr 12, 2023 5:26 pm

Re: Brand New Amscope Phase Contrast Kit

#39 Post by paulg269 » Wed Jul 26, 2023 3:58 pm

Hi Red-Green, I know it’s a while since your original post, but how did the kit turn out? I’m m seriously thinking of buying one and would appreciate your long term thoughts on it. Cheers.

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